好氧反硝化菌BacillusspH2脱氮特性研究张小玲

10。。、。[1-2]。DO＜0.3~0.5mg/LNO2-NO3-N2N2O、NO[1]。NO3-→NO2-→NO→N2O→N2《》[e-mail]。EnvironmentalScience&Technology3410201110Vol.34No.10Oct.2011.Bacillussp.H2[J].2011341053-57.ZhangXiao-lingZhangXia.DenitrificationcharacteristicsofanaerobicdenitrifyingbacteriumBacillussp.H2[J].EnvironmentalScience&Technology2011341053-57.Bacillussp.H2121.4500442.450002、、pH、5Bacillussp.H2。、Bacillussp.H2、、、C/N≥6pHpH7.2~8.230℃2×104cfu/mL48h140mg/L0.14μg·h·cfu－168.1%。X172Adoi10.3969/j.issn.1003-6504.2011.10.0121003-6504(2011)10-0053-05DenitrificationCharacteristicsofanAerobicDenitrifyingBacteriumBacillussp.H2ZHANGXiao-ling1ZHANGXia21.HenanFisheryScienceAcademyZhengzhou450044China2.HenanSoilandFertilizerInstituteZhengzhou450002ChinaAbstractEffectofcarbonsourceC/NratiopHtemperatureandcellconcentrationonthedenitrificationcharacteristicsofanaerobicdenitrifierBacillussp.H2werestudiedinthebatchcultureunderaerobicconditions.ResultsshowedthatthedenitrificationactivityofBacillussp.H2byusingglucosewassignificantlyhigherthanthatbyusingsodiumsuccinateethanolpotassiumsodiumtartratesodiumacetateandthatbyusinglactosewasslightlylowerthanthatbyusingglucose.NearlycompletedenitrificationcanbeobtainedatC/Nm/mratioabove6.AlkalineenvironmentwassuitableforitsgrowthandtheoptimalpHrangewasfrom7.2to8.2.Bacillussp.H2hasthehighestdenitrificationactivitywhentemperaturewas30℃.Nitritenitrogencanberemovedcompletelyanditsdegradationratewas0.14μg·h·cfu－1whichwassignificantlyhigherthanthatofotheraerobicdenitrifiersreportedpreviouslyandtotalnitrogenremovalratewas68.1%whencellconcentrationwasabove2×104cfu/mLwithinitialnitritenitrogenconcentrationof140mg/Lindenitrificationmediumunderlaboratoryconditionsfor48h.KeywordsaerobicdenitrificationBacillusdenitrificationcharacteristics34。NO2-、NO、N2O[3-4]。。Bacillussp.H2[5]。、、pH、、。11.1Bacillussp.H2。1.21.2.1g/L[6]4.72NaNO20.70KH2PO41.50Na2HPO40.42NH4Cl0.610.50MgSO4·7H2O1.02.0mLpH7.2。g/LEDTA50.0ZnSO42.2CaCl25.5MnCl2·4H2O5.06FeSO4·7H2O5.0NH46Mo7O2·4H2O1.1CuSO4·5H2O1.57CoCl2·6H2O1.61pH7.0。1.2.2LBg/L10.05.0NaCl10.0pH7.0~7.2。1×105Pa30min。、6.9×104Pa15min。1.3Bacillussp.H2LB31.0%120r/minDO≥6mg/L29~30℃2dpH、。1.41.4.1、、、、、6。1.2.16C/N＝10：1。。1.32d。4。1.4.21.2.1C/N1：1、4：1、5：1、6：1、7：1、10：1。1.32d。1.4.3pHpH5.5、6.2、7.2、8.2、9.2。pH。1.32d。1.4.41.0%20℃、25℃、30℃、35℃120r/min2d。1.32d。1.4.5103cfu/mL104cfu/mL2×104cfu/mL8×104cfu/mL。1.32d。1.32d。1.5N-1--GB/T7493-1987GB/T11894-1989pHHANNAHI98103。1.6SPSS11.5forWindowsOne-WayANOVADuncan“±”。22.116Bacillussp.H2。99.8%。6≈。P＜0.05。6。Bacillussp.H2。5410OH-pH[6-7]。pH≈＞＞＞≈。pHpH。2.22C/NBacillussp.H2。C/N5：1Bacillussp.H299.6%55.7%。C/N≥6pHBacillussp.H2C/N699.6%68.6%。2.3pH1Bacillussp.H2pH≤5.5。pH7.2~8.298.5%、68.2%。pH9.298.4%、60.9%pH。2.4320℃Bacillussp.H220.9%8.3%。25℃65.9%38.3%。30℃35℃0.2%2.6%P0.05。Bacillussp.H230℃98.3%68.2%。2.54。2×104cfu/mL99.8%68.1%8×104cfu/mL99.7%70.5%2×104cfu/mLP0.05。2×104cfu/mL。1pHTable1EffectofpHondenitrificationP＜0.05。/%/%5.5--6.25.9±0.26a8.3±0.06a7.298.3±0.12b70.8±1.88c8.298.5±0.28b68.2±1.24c9.298.4±0.36b60.9±0.23bBacillussp.H255343。[1]。[8-9]。Bacillussp.H2、。[10]DNF409。[11]D5。[12]Ochrobactrumsp.tbtd。。。[8-913]。。4.6[1]。4.6。6.0~12.5[181014]。。Huang[15]C/N5C.diversus。[16]X315~6。[17]A1C/N5。Bacillussp.H2C/N≥5C/N≥6pHBacillussp.H2C/N6。。、、、pH。pH6.5~7.530~35℃[1]。pH[11-1216-18]。1、3Bacillussp.H2pH7.2~8.27.2~9.2。25~35℃Bacillussp.H2。4~11pH7.5~8.818~35℃Bacillussp.H2。。。。。。[18]W21000cfu/mL1.0mg/L。[10]105cfu/mLDNF4096.4mg/L。[14]9.6×105cfu/mLD3。2×104cfu/mLBacillussp.H248h140mg/L。2。NO2-/NO3-Bacillussp.H2。2Bacillussp.H2Tab.2ComparisonofdenitrificationbyBacillussp.H2andstrains/cfu·mL－1NO2-/NO3-/mg·L-1NO2-/NO3-/%/μg·h·cfu-1Bacillussp.W21×1031.098.80.041[18]Bacillussp.DNF4091×1056.499.90.001[10]D39.6×105277.211.20.032[11]Bacillussp.H22×10414099.80.145610[][1].[M].200455-74.[2].[J].20052810119-123.YeJiang-yuLuoGu-yuanJiFang-yingetal.Functionalmicrobialdiversitiesinwastewaterbiologicaltreatment[J].JournalofChongqingUniversityNaturnalScienceEdi－tion20052810119-123.inChinese[3]MikeSMJettenKuenenJG.HydroxylaminemetabolisminPseudomonasPB16involvementofanovelhydroxy－lamineoxidoreductase[J].AntonievanLeeuwenhoek19977169-74.[4]SuJJLiuBYYangCP.IsolationofanaerobicdenitrifyingbacterialstrainNS-2fromtheactivatedsludgeofpiggerywastewatertreatmentsystemsinTaiwanpossessingdenitri－ficationunder92%oxygenatmosphere[J].JournalofAp－pliedMicrobiology200191853-860.[5].[J].200835101556-1561.ZhangXiao-lingZhangWei-dongZhangLingetal.Selectionofanaerobicdenitrifyingbacteriumanditspre－liminaryapplicationinaquariumwater[J].Microbiology200835101556-1561.inChinese[6]NaokiTMariaABYasushiSetal.Aerobicdenitrifyingbacteriathatproducelowlevelsofnitrousoxide[J].AppliedandEnvironmentalMicrobiology20036963152-3157.[7].[J].2010163394-398.GuoYan-liZhangPei-yuYuDe-shuangetal.Isolationidentificationandcharacteristicsofaslightlyhalophilicden－itrifyingbacterium[J].ChineseJournalofAppliedandEn－vironmentalBiology2010163394-398.inChinese[8]KimMJeongSYYoonSJJetal.AerobicdenitrificationofPseudomonasputidaAD-21atd