好氧反硝化菌的异养硝化性能研究王欢

11*100037。、、。pH7.0DelftiatsuruhatensisPseudomonasputidaBacilluscereusNH4+-NCOD50%。NO2--N。。X830.2A1003-6504(2008)11-0045-04CharacteristicsofHeterotrophicNitrificationbyAerobicDenitrifiersWANGHuanWANGPing*LIUJing-jingSchoolofChemicalandEnvironmentalEngineeringBeijingTechnologyandBusinessUniversityBeijing100037ChinaAbstractHeterotrophicnitrificationcharacteristicsoffiveaerobicdenitrifierswhichbelongtodifferentgenuswerestudied.Alloffivestrainscoulddenitrifypotassiumnitratewithcitrateascarbonsourceandammoniumsulfateasnitrogensource.Afterfivedays’cultureinthemediumfordeterminationofheterotrophicnitrificationremovalefficiencyofNH4+-NandCODforstrainsincludingDelftiatsuruhatensisPseudomonasputidaandBacilluscereuscouldreachmorethan50%.Butthecumulatenitriteoffivestrainswaslittleduringtheculture.Itwasnecessarytojudgenitrificationabilitybyusingcompletenitrogenbalance.OneoffivestrainsDelftiatsuruhatensiscouldaminateacetamidewhenusingasorganicnitrogensource.KeywordsaerobicdenitrifiersheterotrophicnitrificationDelftiatsuruhatensisEnvironmentalScience&Technology3111200811Vol.31No.11No[e-mail]*[e-mail]。。。[1][2]。。-Thiosphaerapantotropha[3]、Alcaligenesfaecalis[4]、HyphomicrobiumX[5]、Pseudomonasstutzeri[6]。。。。11.1SBRApHTIN60.0%~83.03%BpHTIN86%~90%1。DOI:10.19672/j.cnki.1003-6504.2008.11.013311.2NH42SO40.25gKH2PO41gFeCl2·6H2O0.5gCaCl2·7H2O0.2gMgSO4·7H2O1gC6H5Na3O7·2H2O1.3gH2O1000mLpH7.0NH42SO40.35gKH2PO40.75gK2HPO4·3H2O2.216gMgSO4·7H2O0.025gC6H5Na3O7·2H2O1.81gH2O1000mLpH7.01.31.3.150mLLB[8]30℃160r/minDO79%~95%16h5%200mLDM250mL。24hNO3--NNO2--NTINTIN=NO3--N+NO2--N。1.3.250mL30℃160r/min5%200mL。。24hNH4+-NNO3--NNO2--NCODOD600。NH4+-NCOD。1.4[9]NH4+-NNO2--NN-1--NO3--NCODCODOD600721600nm22.11、44dTINNO3--N35%WXZ-1NO3--N100%TIN56%。2.215dNH4+-NCOD。WXZ-1DelftiatsuruhatensisWXZ-6Pseu－domonasputidaWXZ-8Bacilluscereus5dNH4+-N≥50%55.82%51.76%50%COD50%65.24%55.5%69.85%。WXZ-5WXZ-8BacillusWXZ-5BacillussubtilisWXZ-8BacilluscereusWXZ-5WXZ-85dNH4+-N50%50.19%50%。2WXZ-524h48hCODWXZ-8COD。24hNH4+-NCOD。3WXZ-1。OD60024h24h。WXZ-8WXZ-1724hOD6000.871、0.849。1[7]Table1IdentificationresultofexperimentalstrainsWXZ-1WXZ-5WXZ-6WXZ-8WXZ-17112.3WXZ-1。OD600。COD24h4。2.4NO2--NNO3--NNOX--N2。[10]。[11]。Lesley[12]。。-Thiosphaerapantotropha[2]RobersonL.A.Kuenen。31、、DO79%~95%WXZ-1NO3--N100%TIN56%。2COD0～24。WXZ-1DelftiatsuruhatensisWXZ-6PseudomonasputidaWXZ-8BacilluscereusNH4+-NCOD＞50%。。3。4。[][1]HyungseokYooHongKyuAhn.Nitrogenremovalfromsyntheticwastewaterbysimultaneousnitrificationanddeni－trificationvianitriteinanintemittently-aeratedreactor[J].WaterRes1999331145-154.[2]RobersonLAVanNeilEWJTorremansRAMetal.SimultaneousnitrificationanddenitrificationinaerobicchemostatculturesofThiosphaerapantotropha[J].ApplEn－vironMicrobiol198854112812-2818.[3]RobersonLAKuenenJGKleijntjensR.Aerobicdenitri－ficationandheterotrophicnitrificationbyThiosphaerapan－totropha[J].AntonieVanLeeuwenhoek1985514445.[4]Hung-SooJooMitsuyoHiraiMakotoShoda.Characteris－ticsofammoniumremovalbyheterotrophicnitrification-aer－obicdenitrificationbyAlcaligenesfaecalisNo.4[J].JournalofBioscienceandBioengineering20051002184-191.[5]MeibergJBBruinenbergMPMHarderW.EffectofdissolvedoxygentensiononthemetabolismofmethylatedaminesinHyphomicrobiumXintheabsenceandpresenceofnitrateevidenceforaerobicdenitrification[J].JournalofGeneralMicrobiology1980120453-463.1192NOX--NTable2MaxconcentrationofcumulateNOX--Noffiveaerobicdenitrifiers(mg/L)WXZ-1WXZ-5WXZ-6WXZ-8WXZ-17NOX--N0.181.595.667.773.201147[6]SuJJLiuBYLinJetal.Isolationofanaerobicdeni－trifyingbacterialstrainNS-2fromtheactivatedsludgeofpiggerywastewatertreatmentsystemsinTaiwanpossessingdenitrificationunder92%oxygenatmosphere[J].JournalofAppliedMicrobiology200191853-860.[7].[J].2006(7)163-166.XiangMu-feiWangPingLiXiu-tingetal.Selectionandidentificationofaerobicdenitrifiersindenitrificationpro－cessofwastewater[J].FoodScienceandTechnology2006(7)163-166.(inChinese)[8].[J].200730(1)11-13.ZhaiQianWangPingLiXiu-tingetal.Selectionenrich－mentandidentificationofaerobicdenitrifiersinactivatedsludgesystem[J].EnvironmentalScience&Technology200730(1)11-13.(inChinese)[9].[M].2002.StateEnvironmentalProtectionAdministrationofChina.WaterandWastewaterDetectionandAnalysisMethod[M].BeijingChinaEnvironmentalSciencePress2002.(inChinese)[10].[J].2003355378-386.WangYMPengGH.Advanceinmolecularbiologyofheterotrophicnitrifier[J].Soils2003355378-386.(inChinese)[11].[J].2007373486-490.ChenZFYinLHPuYPetal.Screeningofahet－erotrophicnitrifyingbacteriumstrainanditsoptimalcondi－tionsfornitrogenremoving[J].JournalofSoutheastUniver－sityNationalScienceEdition2007373486-490.(inChinese)[12]LesleyARVanNielEWJTorremansRAMetal.SimulataneousnitrificationanddenitrificationinaerobicchemostatcultureofThiosphaerapantotropha[J].ApplEn－vironMicrobiol1988542812-2818.31pH7.5~8.5DO3.5~4.0mg/L4h、2.5~3.0g/L3h、20%~25%。2。COD96.8%COD208mg/L。。[][1].[J].200622956-58.LiuFu-daHeYan-qing.Studyonelectrochemistrypretreat－mentoffarmchemicalwastewaterwithhighconce