好氧反硝化菌群的筛选及其培养条件的研究

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25220106JournalofAnhuiUniversityofTechnologyandScienceVol.25.No.2Jun.,2009:20091109:(08040102002):(1983),,,.:(1956),,,,.:16722477(2010)02003605孙菲1,方金武2,蔡昌凤1*,赵文玲1(1.,241000;2.,241002):A2/O,(DM100)7,,DO=2.5mg/L4,F4F8F9F10.,4,,48hNO-3-N98.75%.4:35,pH=8.0,C/N=5,=25%((2~3)107/mL).,NO-3-N90%96h18h.:;;;:X703.1:A,,[1].,,[24].NO-3NO-2,NO-3NO-2N2ON2[5].,[6].,.:(1),;(2),,-;(3)OH-,pH[7].[89].,,..11.1:(A2/O).:,COD4829.3mg/L,NH+4-N114.85mg/L,NO-3-N7.87mg/L,862mg/L,157mg/L,pH9.0.(DM0):10g/L,10g/L,NaCl5g/L,15~20g/L,,pH7.4~7.6,12120min.DM100.(FM10):13.2g/L,KNO32g/L,K2HPO40.5g/L,MgSO47H2O0.2g/L,FeSO47H2O0.05g/L,CaCl20.02g/L,10%,pH7.5~8.0,11530min(NO-3-N315.47mg/L).FM6060%.1.2(1).150mLFM10,30160r/min48h,10mL3.(2).0.2mLDM0DM100(3),301~3d,DM0DM100,10.(3).,FM10,30160r/min48h96h,NO-3-N,.NO-3-N.(4).:F3F4F5F7F8F9F10DM10DM20DM40DM60DM80DM100,.:FM10,30160r/min24h,12h,NO-3-N.(5).20%FM10,30160r/min24h,NO-3-N.23,4.(6).(20~40)pH(6.5~9.0)C/N(1~7)(5%~40%),.,,FM10,pH=7.5~8.0,35,160r/min,DO=2.5mg/L,(2~3)107/mL.(7).,,,,[10].721600nm,,.:20%120mLFM10,35,160r/min,4h,.22.110,F1~F10.1.DM0DM100.DM0F3,1~2mm;DM100,.1DM0DM100F1(),F2,F3,1~2mm,F4,,F5,,F6,F7,F8,,F9,,F10(),2.2(1).DM100,F7F8DM0,,.NO3-N(1),F6NO-3-N,4.16%.F1F248h96h,NO-3-N,,372,:NO-3-N.F7+F896hNO-3-N98.71%,96hNO-3-N97%F3F4F5F7F8F9F10.(2).2,,NO-3-N,.60%,72h,F3F5F7FM60NO-3-N50%,F4F8F9F1068.95%~71.48%,F4F8F9F10,.2NO-3-N/h/%NO-3-N/%F3F4F5F7F8F9F1024100.886.640.351.613.588.462.77483520.3035.1421.9020.2834.0037.0042.67726047.7668.9543.2336.6871.0068.5571.482.3,,,.,.2,F4F10F4z,OD1.2.34NO-3-N90%,:F4F10F2zF3zF4z48hNO-3-N90%,F9F872h96h.F4zNO-3-N,98.75%1.78.4,.1NO-3-N23NO-3-N2.4(1).,,,[11].20~40.NO-3-N4.4,20~35,40,,,,.35,,18hNO-3-N,67.67%.(2)pH.pH,pH,,.pHNO-3-N5.pH8.0,24hNO-3-N99.43%.pH8.5,,.,,pH,,,.(3)C/N.,,,.,.6,C/N5,18hNO-3-N92.33%,C/N5,C/N,C/N5,NO-3-N,.(4).,NO-3-N3825(7).5%~20%,.25%,18hNO-3-N89.54%,,,OD,.45pH6C/N72.58,.,4:[12].8,F441h,,3,.4,,,.4,.3DM100,..,NO-3-N,,.,35,pH8.0,C/N5,25%((2~3)107/mL).,NO-3-N90%96h18h.:[1],.[J].,2003,28(2):173176.[2],.[J].,1997,14(3):3034.[3],,,.[J].,2006,25(1):3444.[4]ZhangM,TayJH,QianYetal.CokePlantWastewaterTreatmentbyFixedBiofilmSystemforCODandNH3-NRemoval[J].WatRes,1998,32(2):519527.[5],,,.[J].,2007(2):712.[6],,,.[J].,2004,44(6):837849.[7],,,.[J].,2006(7):153155.[8]XieSG,ZhangXJ,WangZS.IntegratedstudyonbiochemicalMechanisminbiofiltersystem[J].ActaSciCircumst,2002,22(5):557561.[9]DingAZ,FuJM,ShengGY.Evidenceofaerobicdenitrification[J].ChinScieBull,2000,45(3):27792782.[10],,,.[J].,2008,36(6):21912193.[11],.[J].(),2003,28(2):173176.[12],,.[M].:,1990.(下转第79页)392,:,,.:[1],,,.[M].:,2001:8790.[2].[J].,2008(2):5457.[3].[M].:,2002:113116.[4].MCS51[M].:.1993:517520.DesignofthecylinderpressuredetectorbasedonSCMYANGLiang,WANGMing(AnhuiProvincialKeyLaboratoryofElectricandControl,AnhuiPolytechnicUniversity,Wuhu241000,China)Abstract:Cylinderpressureisanimportantparameterastheenginerunningnormally.Traditionalanalogpressuregaugeshaveitsowninherentdeficienciessuchaspointerswing,bigerror,etc.Forthisproblem,akindofthedigitalcylinderpressuredetectorusedAT89C52microcontrollerasthecoreisdesigned.Thevariouspartsofhardwarecircuitandthesoftwaredesignareesented.Thedesign,andhasmanyadvantagessuchassmallsizeisoflowcostandsomepracticalvalue,duetouseconventionalIC.Keywords:pressuremonitoring;SCM;A/DConverse(上接第39页)ScreeningofaerobicdenitrifyingbacteriaanddeterminationofthecultureconditionsSUNFei1,FANGJinwu2,CAIChangfeng1,ZHAOWenling1(1.Coll.ofBioch.Engn.,AnhuiPolytechnicUniversity,Wuhu241000,China;2.WuhuXinxingDuctileIronPipesCo.,Ltd,Wuhu241002,China)Abstract:Sevenstrainsdenitrifyingbacteriawereisolatedbybeefextractpeptonemediummixedwiththecokedwastewater(DM100),fromHuaibeiCokingplant'sactivatedsludgeoftheA2/Osewagetreatmentstation.Aftergradientincreaseofcokingwastewater'splatedomesticationandliquiddomestication,fourstrainsaerobicdenitrifyingbacteriawithhighdenitrificationcapacityandresistancetocokingwastewaterwereisolatedinaerobiccondition.(DO=2.5mg/L),namedF4,F8,F9,F10.Thecontrastexperimentshowedthatfourmixedaerobicdenitrificationbacteriahadhigherdenitrificationcapacityandgrewfasterthansinglestrain.Underthesamecondition,fourmixedaerobicdenitrificationbacteria48hNO-3-Nremovalrateis98.75percent.Andthemostsuitablecultureconditionsofthemare:35,pH=8.0,C/N=5,inoculatequantityis25percent(bacteriadensityis2107~3107/mL).Afterconditionsoptimization,thetimeoffourmixedaerobicdenitrificationbacteriaNO-3-Nremovalratewhenattaining90percentreducedform96hto18h.Keywords:aerobicdenitrifyingbacteria;screening;cultureconditions;cokingwastewater792,:

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