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AbstraetsofFoodSummitinChina2009&6thA刀nualMeetingofCIFSTmethod,enzynlatieextraetionmehtodandbio一tarnsfomrationextraetionmethodweersutdiedbythetheaveragemoleeularweihgt,hteaveragemoleeularradiusandhetmolecularstrUcture.Theresultshowedthattheextarctionrateofoatp一glueanwiththeaqueousextraetionmethodwas17.23m留g,theartewihtenz丫rnatieextractionmethodwas19.28m留9andwithbio一transfomrationextraetionmethodwas19.45mg/g.Itindieatedthattheratewithbio一transfomrationextraetionmethodwassimilartothatWihtenyZmatieextraetionmethod,andbothofthemwerehigherhtanhtatwithaqueousextraetionmethod.Theoatp一glucanobatiendwihthetdifferentextaretionmehotdswasPuirifed.hTemoleeularweihgt,radiusandstureutreofP丽ifedp一glueanswasobtainedusinghighPerfomraneegelPemreationehormatograPhy(GpC).Theaveragemoleeularweihgtofp一glueanswithaqueousexatrctionmehtodwas9.697x105,theaveragemoleeularardiuswas83.8nm,andhtestrUetUerwasclosetosPheer;Theaveragemolecularweihgtofp一glueanswihten砂matieextraetionmethodwas1.634x105,thevaeragemoleeularradiuswas31.4unr,andthestureuterwasbewteentosPheerandstick:nAdthevaeragemoleeularweightofp一glueanswithbio一transfomrationexatretionmethodwas1.322x105,theaveragemoleeularardiuswas28.6nm,andhteslureuterwaselosetostick.Keywordsp一glucanOrthogonalexPeirmentAveragemoleeularweight[朝41好氧反硝化细菌的分离及其反硝化能力李秀婷吕跃钢赵进马家津汪苹(北京工商大学化学与环境工程学院北京100048)摘要废水处理过程中脱氮的基础是活性污泥中微生物硝化和反稍化作用。由于峭化和反稍化作用分别为好氧和厌氧过程,因此高效的污水处理过程需进行顺序的有氧和无氧条件控制。为了防止在反稍化作用过程排放大量的NZO,需要利用新型的好氧反峭化细菌建立好氧反峭化过程。本文研究了从活性污泥中分离得到好氧反峭化细菌106及其反消化能力,探讨了培养基碳源、COD夕N、培养温度及pH值对菌株106反峭化作用的影响。结果表明,菌株106能在好氧条件下有效去除培养基中的峭基氮,且其脱氮率达90%以上。其最适培养条件为30℃、摇床转速160mr/in、初始pH.70,分别以唬角酸钠和稍酸钾为唯一碳源和氮源时,最适COD夕N为26。该菌在溶解氧为.60士1.0mg’L一,条件下,培养24h时对NO3一N的去除率为%30%。该反峭化细菌106可以在好氧条件下第一天内完成脱氮,具备高效的反峭化性能,且整个反峭化过程中亚硝酸盐浓度一直保持在极低的水平。关键词好氧反稍化菌生物脱氮分离反稍化作用ISolationandDeintirifcationChaareteirzationofanAerobicDenitiriferXiutingLiYuegangLvJingZhaoJiajinMaPingWang(Se五001ofhCemiealandnEv那onmenatIEnigneenng,Be方ingeTehnol处牙andBusinessnUivesri尔Beljign100048)AbstarctCurrently,nitrogenermovalinwastewateretratment15essentiallybasedontheaetivityofnitirfyinganddenitri尔ngmieroogranisms,bothofhwiehareinhabitantsofaetivatedsludge.EiffeientwastewateretratmenterliesonsueeessivelyexPosingwatertoaeorbieandanaerobiceonditions,sinee110C了石ST一中国食品科学技术学会第六届年会暨第五届东西方食品业高层论坛论文摘要集nitrifieationanddenitrifieationareaerobieandanaerobieProcesses,resPeetively.ToovereomethisPorblemwhieheurrentsystemserleaseeonsidearbleamountsofNZOduringdenitriifeation,novelaerobiedenitirfyingbacteriaareerquierdhtateouldbeusedforeonsturetingaeorbiedenitrifyingPoreesses.Abaeterialstarin106isolatedformtheactivatedsludgewasfoundeaPableofaerobiedeniitrifeationandthedenitirfyingeapabiliytofhtestrainwassutdiedunderaeorbieeondition.Theeffeetsofeabronsouree,CODCr/Nartio,temPerauterandPHvalueondenitirifeationofhteaeorbiedeniitriferweerinvestigated.Theresultsshowedhtathtenitrateintheeulutreeouldbeeiffeieinlyermovedbystrain106andthenitorgenremovalratewasuPtoabove90%·Itshowedthatthenitrite一redueingaetiviyteraehedhtehihgestat30oC,160r/min,PH70andCODCr/Nartio26,usingsodiumsuecinateandPoatssiumniatrteasthesoleeabronsourceandnitrogensoucre,ersPectively.Wllentheeoneenatrtionofdissolvedoxygenwas6.0士l.omg·L一l,theremovalraetofnitratebystrain106weer96.30%in24hours.Ourersultsindicatedhtathtedeniitriferisolatedcanremovehteniotrgenontheifrstday,nearlyeomPletedenitirifeationeanbeobtainedevenunderoxieconditionsandhteerwasaveyrlowlevelofnitriteaeeumulationinhtedeniitrifeationProcess.KeyowdrsAeorbiedeniitrifesrBiologiealniotrgenremovalIsolationDeniitrifcation14451鸡蛋清中卵白蛋白和卵类粘蛋白的高效分离麻小娟’“陈红兵’“高金燕2(’南昌大学食品科学与技术国家重点实验室江西南昌330047;2南昌大学食品系江西南昌330047;’南昌大学中德联合研究院江西南昌330047;)摘要本实验旨在获取卵白蛋白和卵类粘蛋白纯品,为研究鸡蛋过敏提供材料。鸡蛋清经过前处理除去卵粘蛋白后,利用阴离子交换层析柱DEAE一SePharoseFF和舟基磷灰石层析柱iB任GelHTHydroxyaPiatet,通过调节洗脱速度,洗脱液pH值等洗脱条件,分离卵白蛋白和卵类粘蛋白。结果显示,一次性上样1200mg卵清蛋白质,分离得到卵白蛋白570mg,卵类粘蛋白57mg,经SDS一PAGE电泳比对,所得两种蛋白质纯度均高于标准品(卵白蛋白98%,SIGMA公司;卵类粘蛋白90%,sIGMA公司),回收率分别为88%和43%。综上分析,鸡蛋清中蛋白质组成主要为:54%卵白蛋白(p.4I5),12%卵转铁蛋白(pI6.0),11%卵类粘蛋白(p4I.l),3.4%溶菌酶(pllo.7),3%卵粘蛋白(p4I.7)和多种球蛋白。前处理可除去卵粘蛋白和多种球蛋白,而溶菌酶和卵转铁蛋白与两目的蛋白的等电点相差较大,通过阴离子交换层析很容易分开。舟基磷灰石层析柱含有阳离子C+az和阴离子PO护一两种离子的交换作用,通过精细的阴阳离子结合分离机制,最终使卵白蛋白和卵类粘蛋白分离开来。关键词卵白蛋白卵类粘蛋白分离纯化食物过敏EiffeientSeParationOfHenEggWhietOvalbuminandOvomucoidXiaojuanMa,,,HonghingChen,,,JinyanGao,(`SatetKeyaLbaortoyrofoFodSeieneeandeTehnology{aNnchagnnUiverslyt,aNnehagn330047,以ina;勺叩art川entofoFdocsi朗ce,aNn动agn饰i~i仪aNn动ang33口047,hCin炙JSin。一Gen刀anoJintResaecrh切sit勿et,入alnhcagnnUiversiyt,aNnehang330047,山ina;)AbstarctThePuprosewasotgetPuriifedovalbuminandovomueoidfortheheneggallergysutdy.111