好氧反硝化细菌的筛选及其在鸡粪发酵氮素转化中的作用

8,7(6):2494-2498-3SF35-1JF45-2JF35-13SF35-1JF45-2X172A1672-2043(2008)[e-mail]150030ScreeningofAerobicDenitrifiersandTheirFunctioninNitrogenTransformationofAerobicCompostingofChickenManureWANGLi-qun,XIAOWei-wei,CAOLi-qun,YUQi-lin,SUNWen,GUWen-jieCollegeofLifeSciences,NortheastAgriculturalUniversity,Haerbin150030,ChinaAbstractAerobicdenitrifyingbacteriawereisolatedatdifferenttemperaturestagesincompostingofchickenfeceswithstrawasconditioner.Thedominantstrainswerescreenedbymeasuringde-nitrificationability.Thenthedominantstrainswerere-culturedintothecompostingofchickenfecesandthecontentsofammonianitrogen,nitratenitrogenandtotalnitrogenweremeasured.Theresultswereasfollowstherewereaerobicde-nitrifiersinthecompostingofchickenfeces.Threedominantstrains,namedSF35-1,SF45-2andJF35-1,werescreenedbydeterminingactivityofnitrogenremoval,andidentifiedasBacillussp.,Derxiasp.andKlebsiellasp.respectively.Thethreestrainswereallisolatedfrommid-temperaturestage.Itshowedthatde-nitrificationwasaffectedremarkablybytemperatureandwasstrongerinmid-temperaturestagethanotherstages.TheSF35-1Bacillussp.andJF45-2Derxiasp.alsohadtheabilityofnitrification,whichwasweakerthantheabilityofde-nitrification.Thedominantaerobicdenitrifyingbacteriainoculatedintocompostresultedinreductionofnitratenitrogenandtotalnitrogencontents,andsuggestedthatdenitrifyingbacteriahadde-nitrificationinchickenfecesfermentation.Itwouldreducethenitrogenlossandairpollutionbyeffectivelyregulatingtheactivityofthisgroupofbacteria.Keywordsaerobicdenitrifiers;compostingofchickenmanure;nitratepHN2N2O[1-2][3][4]76Patureau[5][6][7]11.11.1.1[8]1.1.211.62.1C/N25651.1.3FOSS2100ZHWY211B1.21.2.18mgL-165102535455565453571.2.21.2.34%50mL250mL3130rmin-1[9]-[10]1.2.4112%[11]-[11]-[11]1.38[12]22.11296h1SF35-1JF45-2JF35-182mgL-1P0.01SF25-3JF55-1JF35-2354555SF55-132.5mgL-1652.22SF35-1024h2495811105.0mgL-172h96h33.5%48hJF45-2024hSF35-1160.4mgL-148h96h44.4%048hJF35-1048h96h30.6%3SF35-1JF45-224h24hRobertsonScholten[13-14]2.3316d06d12d0.15gkg-10.24gkg-14356d0.11%23Figure2Nitrogenremovalofthethreedominantdenitrifyingbacteria24967613Table1ResultsofphysiologicalandbiochemistryexperimentsofthethreedominantdenitrifyingbacteriaSF35-1JF45-2JF35-1SF35-1JF45-2JF35-1M.R.++-+--V.P.+-++--0.5%NaCl+++--+2%NaCl+++---7%NaCl+-+---10%NaCl-----+++++++---+++--+--+---+-+-----+-+---+-----+2.4183SF35-1JF45-2JF35-1BacillusDerxiaKlebsiella313SF35-1JF45-2JF35-1BacillusDerxiaKlebsiellaSF35-1JF45-223[1]TiquiaSM,TamNFY.Fateofnitrogenduringcompostingofchickenlitter[J].EnvironmentalPollution,2000,110535-541.[2]EklindY,KirchmannH.CompostingandstorageoforganichouseholdwastewithdifferentlitteramendmentsNitrogenturnoverandlosses[J].BioresourceTechnology,2000,74125-133.[3]OslisoA,LewandowskiZ.Inhibitionofbyselectedorganiccompounds[J].WaterResearch,1985,194230-4236.[4],,.[J].,2005,241165-168.ZHANGYu-qin,LIUKai-qi,WANGGe.Screeningofdenitrifyingbacteriaanddeterminationofthecultureconditions[J].JournalofAgro-environmentalScience,2005,241165-168.[5]PatureauD,BernetN,etal.Effectofdissolvedoxygenandcarbonnitrogenloadsondenitrificationbyanaerobicconsortium[J].AppliedMicrobiologyandBiotechnology,2000,54535-542.[6],,,.[J].,2007,111-13.ZHAIQian,WANGPing,LIXiu-ting,etal.Selection,enrichmentandidentificationofaerobicdenitrifiersinactivatedsludgesystem[J].EnvironmentalScience&Technology,2007,111-13.[7]ZhouLi-xiang,HuangFeng-yuan,WangShi-mei.Isolationofaerobicdenitrifiersandtheirrolesinsoilnitrogentransformation[J].ActaPedologicaSinica,2006,433430-435.[8],,.[J].,2005,20280-83.MAFang,WANGHong-yu,ZHOUDan-dan.Selectionandenrichment+-2497811ofaerobicdenitrifierinactivatedsludgesystem[J].JournalofHunanScienceandTechnologyUniversity,2005,20280-83.[9],.[J].,2002,226107-110.BAILan,DUJi-yu.Determinationofnitratenitrogeninvegetable[J].Agriculture&Technology,2002,226107-110.[10]..[D].2004.CAOXi-tao.Nitrogenlossesandinoculationwithheterotrophicnitrifierduringthecompostoflivestockandpoultrymanure.MasteralDissertationofNanjingUniversityofAgriculture[D].NanjingNanjingUniversityofAgriculture2004.[11],.[M].,2000.XUJing-an,PANZhen-yu.Analysisandmeasurementmethods[M].BeijingChemicalIndustryPress,2000.[12].[M].,1978.InstituteoftheChineseAcademyofSciencesMicrobialBacteriaClassificationunit.Themethodsfornormalbacteria[M].BeijingSciencePress,1978.[13]RobertsonLA,KuenenJG.Combinedheterotrophicnitrificationandaerobicdenitrificationinthiosphaerapantotrophaandotherbacterial[J].An.VanLeeuwenhoek,1990,56289-299.[14],,.[J].,2001,27122-24.LICong-na,LVXi-wu,etal.Studyonnitrogenremovalbysimultaneousnitrificationanddenitrification[J].Water&WastewaterEngineering,2001,27122-24.500,CAAJ71621640.00480.006643001913102223674336,[e-mail]