好氧反硝化在短程硝化反硝化工艺中的作用研究

吴成强1,2,　杨　清3,　杨　敏2(1.浙江工业大学生物与环境工程学院,浙江杭州310032;2.中国科学院生态环境研究中心,北京100085;3.济宁职业技术学院汽车工程系,山东济宁272037)　　　:　采用SBR反应器处理垃圾渗滤液,研究了短程硝化反硝化过程中好氧反硝化的作用。结果表明,SBR反应器的亚硝化效果良好,氨氮几乎完全被氧化为NO-2-N;该系统的活性污泥中同时存在能还原NO-3-N和NO-2-N的好氧反硝化菌,还原NO-3-N的好氧反硝化菌和氨氧化菌的数量及其总活性高于NO-2-N氧化菌,这是SBR反应器能够长期维持亚硝化状态的重要原因;有机物浓度越高则好氧反硝化速率越快,此时氨氮均被氧化为NO-2-N,当有机物浓度达到某临界值时,好氧反硝化速率几乎保持不变;溶解氧浓度越低则好氧反硝化速率越快,释放出的OH-会导致pH值升高。好氧反硝化对于维持和促进SBR反应器的短程硝化反硝化具有重要的作用。　　:　短程硝化反硝化;　好氧反硝化;　SBR:X703.1　　:C　　:1000-4602(2007)23-0097-04RoleofAerobicDenitrificationinShort-cutNitrificationandDenitrifi-cationProcessWUCheng-qiang1,2,　YANGQing3,　YANGMin2(1.CollegeofBiologicalandEnvironmentalEngineering,ZhejiangUniversityofTechnology,Hangzhou310032,China;2.ResearchCenterforEco-EnvironmentalSciences,ChineseAcademyofSciences,Beijing100085,China;3.DepartmentofAutomobileEngineering,JiningVocationalCollegeofTechnology,Jining272037,China)　　Abstract:　TheSBRwasusedtotreatlandfillleachate,andtheroleofaerobicdenitrificationinshort-cutnitrificationanddenitrificationprocesswasstudied.Theresultsshowthatthenitrosationeffi-ciencyintheSBRispreferable,andammonianitrogeniscompletelyoxidizedtoNO-2-N.TheaerobicdenitrifiersabletoreduceNO-3-NandNO-2-Nco-existintheactivatedsludge,andtheamountandtotalactivityoftheaerobicdenitrifiersreducingNO-3-Nandammoniaoxidizersarehigherthanthoseofnitriteoxidizers,whichisanimportantreasonwhytheSBRcanmaintainnitrosation.Theorganicssignifi-cantlyincreasetheaerobicdenitrificationrate.AmmoniaisoxidizedtoNO-2-N.Theaerobicdenitrifica-tionratekeepsconstantwhentheorganicconcentrationreachesacriticalvalue.AtthelowerDOlevels,theaerobicdenitrificationrateincreases,andthereleasedOH-cancausetheriseofpH.Therefore,theaerobicdenitrificationplaysanimportantroleinmaintainingandpromotingtheshort-cutnitrificationanddenitrification.　　Keywords:　short-cutnitrificationanddenitrification;　aerobicdenitrification;　SBR　　2080,,·97·第23卷　第23期2007年12月　　　　　　　　　　　　中国给水排水CHINAWATERWASTEWATER　　　　　　　　　　　　　Vol.23No.23Dec.2007[1～6]。NO-2-N()NO-3-N,N2,。SBR,、pH、DO,,。1　试验装置与方法1.1　SBRSBR4,6h,1h,5h。3.2L,HRT3.2d,8～10g/L。,10min,。,,NO-2-N。1.2　SBR,500mL,,,,。,,。,(、),15min。,(600mg/L)。1.3　SBRCOD4000mg/L。:COD2400～2800mg/L,NH+4-N3100～3800mg/L,TN4100～4800mg/L,pH8.4～9.0,14000～16000mgCaCO3/L。1.4　COD:;NH+4-N:—;TN:—;NO-3-N、NO-2-N:;DO、pH:。2　结果与讨论2.1　SBRSBR,DO,1mg/L。,,NO-2-N,NO-3-N10mg/L。SBR、1。1　SBRTab.1　InfluentandeffluentqualityofSBRreactor/(mg·L-1)/(mg·L-1)/%CODNH+4-NTNCODNH+4-NNO-2-NTNNH+4-NTN327517942194933508488101371.6846.17　　1,,71.68%,TN46.17%。,1gTN1.93gCOD,A/O[7]。,,。2.2　Frette,NO-3-NN2ONO-2-N3.4[1],NO-3-N。25℃、DO8～9mg/L、pH8.3、TS1.52g/L,1。1　Fig.1　Variationofnitrogenconcentration·98·第23卷　第23期　　　　　　　　　　　　　　中国给水排水　　　　　　　　　　　　　　1,DO,NO-3-NTN,NO-2-N,,。,,,,NO-3-NNO-2-N。2h,NO-3-N,NO-2-N,;2h,NO-2-N,NO-2-NNO-2-N,NO-2-NNO-3-N。,NO-2-NNO-3-N,,NO-2-N。SBR,NO-3-NNO-2-N;NO-2-NNO-2-N。SBRNO-2-N。NO-3-N,SBR,NO-2-N。,NO-2-N。SBR,NO-3-N,,SBR。,3(NH+4-N=252mg/L,NO-2-N=109mg/L,COD=275mg/L)(OUR),[8]。,OUR=15.21mgO2/(gTSS·h),OUR=1.73mgO2/(gTSS·h),9,SBR。2.3　2()。2　Fig.2　Effectofaerobicdenitrificationatdifferentsugarconcentration　　,TN,TN,,。,,,TN。,,NO-2-N,NO-3-N;,0.1gNH+4-N/(gTS·d),。,,。(COD=840mg/L),9hTN32%;(COD=1028mg/L),9hTN11.7%。,,。2.4　DO,;4h,,。DO0.8mg/L,,DO=9.5mg/L4.6。RobertsonThio-sphaerapantotrophaDO=5.6mg/L,2,。,,·99·吴成强,等:好氧反硝化在短程硝化反硝化工艺中的作用研究第23卷　第23期,。,DO,pH。DO0.8mg/L,6hpH9.4,。,,pH。3　结论①　SBR,。②　NO-3-N,。③　,,,NH+4-NNO-2-N;。:[1]FretteL,GejlsbjergB,PeterW.Aerobicdenitrifiersisola-tedfromanalternatingactivatedsludgesystem[J].FEMSMicrobiolEcol,1997,24(4):363-370.[2]PatureauD,BernetN,MolerraMR.Combinednitrifica-tionanddenitrificationinasingleaeratedreactorusingaerobicdenitrifierComamonassp.strainSGLY2[J].WaterRes,1997,31(6):1363-1370.[3]　PatureauD,BernetN,BouchezT,etal.Biologicalnitro-genremovalinasingleaerobicreactorbyassociationofanitrifyingecosystemtoanaerobicdenitrifier,Microvirgulaaerodenitrificans[J].JMolCatalB:Enzym,1998,5:435-439.[4]　PatureauD,HelloinE,RustrianME,etal.Combinedphosphateandnitrogenremovalinasequencingbatchre-actorusingtheaerobicdenitrifier,Microvirgulaaerodeni-trificans[J].WaterRes,2001,35(1):189-197.[5]　GuptaAB,GuptaSK.SimultaneouscarbonandnitrogenremovalfromhighstrengthdomesticatedwastewaterinanaerobicRBCbiofilm[J].WaterRes,2001,35(7):1714-1722.[6]　,,,.[J].,2001,17(5):24-28.[7]　.[M].:,2000.[8]　Surmacz-GorskaJ,GernaeyK,DemuynckC,etal.Nitrifi-cationmonitoringinactivatedsludgebyoxygenuptakerate(OUR)measurements[J].WaterRes,1996,30(5):1228-1236.:[phone]::2007-06-20(上接第96页):[1]　WeemaesMPJ,VerstraeteWH.Evaluationofcurrentwetsludgedisintegrationtechniques[J].JChemTechn-olBiotechnol,1998,73(2):83-92.[2]　,,.[J].,2005,25(9):16-19.[3]　MullerJA.Prospectsandproblemsofsludgepre-treat-mentprocess[J].WaterSciTechnol,2001,44(10):121-128.[4]　LinJG,ChangCN,ChangSC.Enhancementofanae-robicdigestionofwasteactivatedsludgebyalkalinesolu-bilization[J].BioresourTechnol,1997,62(3):35-42.[5]　,.[J].,1997,23(1):10-15.[6]　TanakaS,KoshioK,KamiyamaK,etal.Effectofther-mochemicalpretreatmentontheanaerobicdigesti