好氧饥饿对膨胀污泥硝化性能及污泥特性的影响

Seediscussions,stats,andauthorprofilesforthispublicationat:ficationperformanceandsludgecharacteristicsofbulkingsludgeArticle·March2015DOI:10.11949/j.issn.0438-1157.20141138CITATION1READS1655authors,including:WenlongLiuHarbinInstituteofTechnology5PUBLICATIONS   9CITATIONS   SEEPROFILEAllcontentfollowingthispagewasuploadedbyWenlongLiuon18January2016.Theuserhasrequestedenhancementofthedownloadedfile.20153CIESCJournal1142March2015663Vol.66No.3(100124)SBR/14dammonia-oxidizingbacteria,AOB[0.420.06d1nitrite-oxidizingbacteria,NOB[0.340.05d1]AOBAOBNOBSVI170mlg130mlg1extracellularpolymericsubstances,EPS(solublemicrobialproducts,SMP)DOI10.11949/j.issn.0438-1157.20141138X703A04381157201503114208EffectsofaerobicstarvationonnitrificationperformanceandsludgecharacteristicsofbulkingsludgeLIUWenlongPENGYongzhenMIAOYuanyuanLIZhongmingWANGShuying(KeyLaboratoryofBeijingforWaterQualityScienceandWaterEnvironmentRecoveryEngineering,EngineeringResearchCenterofBeijing,BeijingUniversityofTechnology,Beijing100124,China)AbstractTheeffectsofaerobicstarvationonnitrificationbacteria’sactivityandsludgeperformanceoffilamentousbulkingsludgewereinvestigatedinanaerobic-anoxicSBRprocess.Ammonia-oxidizingbacteria(AOB)hadahigherhungrysensitivitythannitrite-oxidizingbacteria(NOB)in14daysofaerobicstarvationprocesswhichwaskeptaerationandwithoutfeed.ThedecayofAOB[(0.420.06)d1]washigherthanNOB[(0.340.05)d1].Thephenomenonofnitriteaccumulationwasapparentlyachievedfromthesystemintheearlyresuscitationperiod.ThisphenomenonwasduetothatAOBwasabletokeeptheircellsinastateofreadinesswhereitwaspossibletostartoxidizingammoniaalmostimmediatelyandwiththemaximumrateaftershorterstarvationperiods.Theyweretherebyabletorespondrapidlytochangingenvironmentalconditions,whichwasreflectedbythehigheractivityresuscitationrateofAOBthanthatofNOB.Inaddition,aerobicstarvationcouldkillfilamentousbacteriaandimprovethesettleabilityoffilamentousbulkingsludgequickly.Andsludgevolumeindex(SVI)accordinglydeclinedfrom170mlg1to30mlg1.Extracellularpolymericsubstances(EPS)and2014-07-292014-09-051990—2012ZX07302002-06Receiveddate:2014-07-29.Correspondingauthor:Prof.PENGYongzhen,[e-mail]31143solublemicrobialproducts(SMP)couldtransformintoeachotherandbecamethesourceofcarbonandenergyforhungrybacteriatoguaranteecellmaintenanceinalongtermstarvationenvironment.Keywordsaerobicstarvationbulkingsludgenitritationresuscitationdecayratesludgemorphology[1][2][3][4]AOBNOBNH+4NO2NO2NO3Salem[5]Siegrist[6]/[7]Pijuan[8]/AOBNOB1.1A/OC/N=3.0254L0.19kgNm3d1SVI28130mlg1FI=31.23L10LSBR10LSBR1d118h3LSBR3LSBR1d46h1.314d5200mgL11d100mlAOBNOBDO4.940.5mgL1281pH7.85.025.60.514d3LSBR1.21d100mlAOBNOB2h32.5d1.4NH+4-NNO2-NNO3-NQuikChem8500LACHAT661144MLSSMLVSSSVIAPHA[9]pHDOWTWMulti340ispecificoxygenuptakerateSOURStrathtoxFEIQUANTA200,FEICompanyinUSA[10]2.5%pH=6.81.5h0.1molL150%70%80%90%100%EPSSMPTOCMultiN/C3000TN/TOC()EPS[11]SMP0.45mfolin-DNA1.5AOBNOB100ml40005min3400ml500ml2hDO=4.000.050.1molL1HCl0.1molL1NaOHpH7.50.0520mgL1NO2-NNOB1h30mgL1NH+4-NAOB3OUR1OUR2NOB30minOUR3AOBNOB90min10min10mlNH+4-NNO2-NNO3-NAOBNOBspecificammoniaoxidationrateSAOR(specificnitriteoxidationrateSNOR)AOBOUR3OUR2NOBOUR2OUR1bd0lntRbtRR0Rttddbd1R0Rttddbd11.6[12]FISHEUBmixEubacteriaNSO1225ammoniaoxidizingbetaproteobacteriaNIT3NitrobacterNtspa662Nitrospira22.12.1.1硝化性能的恢复与亚硝酸盐的积累1160ammoniaremovalloadARL10.66gNm3h1AOB1Fig.1Nitrificationperformanceofsystembeforeandafteraerobicstarvation31145[NAR,NAR=(NO2-N/NOx-N)×100%,NOx-N=NO3-N+NO2-N]11%14d3d(0.680.5)gNm3h19d9.55gNm3h110d60%14dSBRAOBNOBMorgenroth[13]Pijuan[8]FISH14dAOB2.1%1.1%Nitrospiraspp.Nitrobacterspp.3.3%2.4%1.6%1.0%AOB7d2.0%Nitrospiraspp.Nitrobacterspp.NOB7d2.1.2AOB与NOB的衰减情况2AOBNOB14d2ab2a2dAOB0.88mgN(gMLSS)1min10.28mgN(gMLSS)1min168%NOB0.97mgN(gMLSS)1min10.427mgN(gMLSS)1min156%AOB[0.420.06d1]NOB[0.340.05d1]1AOBNOBSalem[5]30dAOBNOB0.200.016d10.210.024d1Hao[14]7dAOB[0.1440.008d1]NOB[0.3060.026d1]NOBAOB2AOBNOBFig.2DecaycurveofAOBandNOBunderaerobicstarvation661146AOBAOBAOB2.1.3AOB与NOB的活性恢复Morgenroth[13]Bollmann[17]AOBAOB3AOBNOB9d29d13dAOBNOB9dAOB[0.290.08d1]NOB[0.160.05d1]10d60%Tappe[2]311dAOB2AOBNOBTable2ActivityrecoveryratesofAOBandNOBResuscitationperiodTypeofbacteriaActivityrecoveryrates/d1SAORorSNORSOUR9dAOB0.290.080.290.03NOB0.160.050.150.0313dAOB0.220.040.200.03NOB0.200.020.260.071AOBNOBTable1DecayratesofAOBandNOBunderaerobicstarvationinliteratureandthisstudyTypeofsludgeStarvationperiodStarvationconditionsActivitytestmethodDecayrates/d1Ref.pHTemperature/AOBNOBenrichedcultureofnitrifier30d7.520nitrificationbatchtest0.020.0020.080.000[5]activatedsludge30d7.520nitrificationbatchtest0.200.0160.210.024[5]purecultureofN.europaea14d7.430SOUR0.43[15]partialnitrificationactivatedsludge30d8.59.0182SOUR0.240.02[16]enrichedcultureofnitrifier7d7.30.05300.5SOUR0.1440.0080.3060.026[14]bulkingsludge14d5.60.5281nitrificationbatchtest0.420.060.340.05thisstudybu