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25420067ENVIRONMENTALCHEMISTRYVo.l25,No.4July20062005725.*(2002DF000006).**:051085888301,Emai:ljchen@sytueducn*李延军1李秀芬1华兆哲1刘和1陈坚1,2**(1,,214036;2,,214036)(EPS).:,EPS.,EPS,EPS.EPS,C/N.45mgl-1,pH,037kgCODkg-1MLSSd-1,201,EPS9%12%,(06%20%),(80%),,EPS,1426mgl-1.,,,.,,(extracellularpolymericsubstances,EPS)[13],,[46],,EPS.EPS,EPS,,EPS.EPS,,.111,,.5,UASB.1035mm,60100mms-1,SVI420mlg-1.337mgg-1MLSS,939mgg-1MLSS.12500ml25150rmin-1.,12h,115h,05h.EPS,EPS.3,.(1)100m,l200m,l300m,l400m,l450ml500ml,DO71mgl-1,66mgl-1,58mgl-1,45mgl-1,38mgl-107mgl-1,DOEPS.:pH7075,(MLSS)5000mgl-1,C/N20.(2)HClNa2CO3pH,pH55,7085EPS.:DO58mgl-1,MLSS5000mgl-1,C/N20.(3),MLSS1000mgl-1,2000mgl-1,3000mgl-1,5000mgl-1,7000mgl-1,9000mgl-1110000mgl-1,MLSS44025EPS.:DO58mgl-1,pH7075,C/N201.(4)NH4Cl.10,13,20,3060,C/NEPS.:DO58mgl-1,pH7075,MLSS5000mgl-1.221DOEPS1(A)EPSDO.DO,EPS,23.DO45mgl-1,EPS901mgg-1MLSS,90%,(0620%),80%[7].EPS1(B).DO45mgl-1,EPSDO,07mgl-1173mgl-1,110mgl-1282mgl-145mgl-163mgl-1,103mgl-1166mgl-1,,,.,DO.Beun[8]SBAR,DO10mgl-1,.,O2NO-3,,,NO-3,NO-3,.,DO07mgl-1,EPS,282mgl-1.Beun,EPS.DO`,EPS,.:,DO,EPS;,DO,,,,EPS,,EPSDO.DO(45mgl-1),DO,,EPS,,,DO45mgl-1,EPS,166mgl-1.,EPS,,EPS.1DOFig1TheeffectofEPSinstudgeandsupernatantatdifferentDOconcentration22pHEPSpHEPS2.,pH70EPS4:441,1027mgg-1MLSS,EPS103%,,3.pH5585,EPS,,,.EPS,EPS,5276mgl-1,30,160mgl-1,EPS.,,,.,,pH/.,,,,,.2pHFig2TheeffectofEPSinstudgeandsupernatantatdifferentpHvalue[9]pH,pH,,,,EPS,,pH,,,,.2,,,EPS,EPS(),,,EPS.23EPS3,256kgCODkg-1MLSSd-1(MLSS1000mgl-1)021kgCODkg-1MLSSd-1(MLSS11000mgl-1),EPS,200mgg-1MLSS.EPS.,MLSS,EPS,EPS[10],,EPS.,MLSS,EPS.MLSS(1000mgl-1),,,EPS,MLSS,,EPS.MLSS,EPS,EPS,EPS.EPSMLSS2000mgl-1.MLSS7000mgl-1,EPS.037kgCODkg-1MLSSd-1,EPS263mgl-1,EPS1181mgg-1MLSS,118%.EPS.,EPS.442253MLSSFig3TheeffectofEPSinstudgeandsupernatantatdifferentMLSSconcentration24C/NEPSC/NEPS4(A).,,EPSC/N,C/N60,EPS,935mgg-1MLSS,Durmaz[11].C/NEPS,C/N,EPS.4(B)C/NEPS.,EPSC/N,,C/N20,139mgl-187mgl-1,EPS1235mgg-1MLSS,124%.:C/NEPS,,EPS,EPS,EPS.4C/NFig4TheeffectofEPSinstudgeandsupernatantatdifferentC/Nratio3(1)(pH55)EPS,,EPS.,C/N.(2)DO45mgl-1pH037kgCODkg-1MLSSd-1C/N4:443201,EPS9%12%,(06%20%),80%,EPS,1426mgl-1.[1],,,.,2004,23(3)252257[2],,,.(),2004,44(6)766769[3],,,.,2002,21(6)546551[4],,.,1999,20(2)3841[5],,,.,2003,24(4)99104[6],,,SBAR.,2004,24(6)725729[7]MagaraY,NumbuS,UtosawaK,BiochemicalandPropertiesofanActivatedSludgeonSettlingCharacteristics[J].Wat.Res.,10(1)7177[8]BeunJJ,HendriksA,VanloosdrechtMCMeta.l,AerobicGranulationinaSequencingBatchReactor.Wat.Res.,1999,33(10)22832290[9],,,.,2004,5(5)2125[10],,,Ca2+pH.,2004,24(4)437441[11]DurmazB,SaniFD,EffectofCarbontoNitrogenRatioontheCompositionofMicrobialExtracellularPolymersinActivatedSludge.WaterSci.Technol.,2001,44(10)221229PRODUCTIONOFEXTRACELLULARPOLYMERICSUBSTANCESFROMAEROBICGRANULARSLUDGEANDITSDISTRIBUTIONLIYanjun1LIXiufen1HUAZhaozhe1LIUHe1CHENjian1,2(1SchoolofBiotechnology,SouthernYangtzeUniversity,Wux,i214036,China)(2KeyLaboratoryofIndustrialBiotechnology,MinistryofEducation,SouthernYangtzeUniversity,Wux,i214036,China)ABSTRACTTheeffectofoperatingandnutritionconditionsonthemassofextracellularpolymericsubstances(EPS)producedbyaerobicgranularsludgeanditsdistributionbetweensludgeandsupernatantwasinvestigatedinthepresentpaper.TheresultsshowedthatthechangesofEPScontentproducedanditsdistributionwereregularwiththeoperatingandnutritionconditions.TheamountofEPSinsludgewasrelativelystableandtheexcessiveEPSwouldbereleasedintothesupernatan.tMoreover,alargeamountofEPSwasproducedonlywhentheaerobicsludgegranularbroke.pHvalueandC/Nratiohadbadeffectonthegranulationofsludge.Undertheconditionsof45mgl-1DO,70pHvalue,037kgCODkg-1MLSSd-1sludgeloadingand201C/Nratio,thecontentofEPSinaerobicgranularsludgewasaround9%12%,whichfellinto06%20%ofanaerobicgranularsludgeandmuchlowerthan80%ofaerobicflocsludge,andtheconcentrationofEPSinsupernatantamountedtothelowestwith1426mgl-1.Keywords:aerobicgranularsludge,extracellularpolymericsubstances,protein,polysaccharide.