好氧颗粒污泥的快速培养与污泥特性分析王昌稳

446()Vol.44No.620136JournalofCentralSouthUniversity(ScienceandTechnology)June20131111,21(1.1001242.250101)5min(EPS)7min5min(VSS)(140.9819.54)mg/g(310.7950.86)mg/g5minX703.1A1672−7207(2013)06−2623−06RapidcultivationofaerobicgranularsludgeandanalysisofsludgecharacteristicsWANGChangwen1,LIJun1,ZHAOBaihang1,WANGYonglei1,2,LIUGuoyang1(1.KeyLaboratoryofBeijingforWaterQualityScience&WaterEnvironmentRecoveryEngineering,BeijingUniversityofTechnology,Beijing100124,China;2.CollegeofMunicipalandEnvironmentalEngineering,ShandongJianzhuUniversity,Jinan250101,China)Abstract:Inordertoexploretheeffectofdifferentsettlingtimesonaerobicgranulation,testwascarriedoutinasequencingbatchreactor(SBR),whereaerobicgranularsludgewasrapidlyculturedthroughshorteningsettlingtimestepbystep.Theresultsshowthatduringtheprocedure,themixedliquorsuspendedsludgedecreasedgradually,while,thesludgesettleabilitywasimprovedandtheinorganiccontentinthesludgeincreased.Thesludgeparticlesizedifferedunderdifferentsettlingtime,andtheaverageparticlesizehasagoodnegativecorrelationwiththesettlingtime.Moreover,aerobicgranularsludgewillformifthesettlingtimeisshorterthan5min.Thepolysaccharideplaysamainroleinsludgegranulation.Duringthegranulationprocedureinwhichthesettlingtimeisshortenedfrom7minto5min,thepolysaccharideinextracellularpolymericsubstances(EPS)increasesfrom(140.98±19.54)mg/gto(310.79±50.86)mg/g.Shorteningthesettlingtimeisaneffectivestrategytocultivateaerobicgranularsludgeinasequencingbatchreactor,andthesettlingtimerequiredforsuccessfulaerobicgranulationwillnotbelongerthan5min.Keywords:sequencingbatchreactor;aerobicgranularsludge;settlingtime;extracellularpolymericsubstances                                  2012−06−052012−09−14(51078008)(8122005)(KZ201110005008)(ykj-2012-6867)(1964−)[e-mail]()442624(aerobicgranlularsludge,AGS)1991Mishima[1][2−5][6]Morgenroth[7](sequencingbatchreactorSBR)SBR11.1SBRSBR120cm18cm30.52L25.43L20cm1L/h25~28SBR35min7.5h(15min2min)5min5min60%10h1.2ρ(COD)250mg/Lρ(NH4+-N)100mg/L1A2OMLSS3780mg/L(SVI)981.3CODNH4+-NNO3−-NNO2−-NMLSS[8]BX41Mastersizer200025%1.5hPBS350%70%80%90%100%10~15min371500nmHitachiS−4300EPS50mL4000r/min5min8030min5000r/min30min0.22μmEPS(PN)(PS)PNPS−1Table1Compositionsofartificialwastewater/(g·L−1)/(mg·L−1)NaAc0.32H3BO3150NH4Cl0.46CuSO4·5H2O50KH2PO40.04KI150MgSO4·7H2O0.02MnCl2·4H2O110CaCl2·2H2O0.01CoCl2·6H2O150FeCl3·6H2O1.5Na2MoO4·2H2O60ZnSO4·7H2O1200.50mL/L22.1105.23μm(1(a))[9]10min(1(b))187min62625(a)(b)10min(c)(d)1Fig.1Opticalmicrographsofactivatedsludgeindifferentstages480.74μm3min1636.56μm(1(c)(d))SVI63Liu[10]4(1)(2)()()()(3)EPS(4)1(a)(b)22(2(a))5000(a)(500)(b)(5000)2Fig.2Scanningelectronmicrographsofaerobicgranularsludge()442626(2(b))(EPS)2.215minSVI(MLSS)(MLVSS)33(MLSSMLVSS)(SVI)[11−12]3minMLSS1600mg/LSVI63(MLVSS)(MLSS)(0.780.44)MLSSSVI23[13−14]Lei[15]SBR(VS)(TS)0.880.532.3Mastersizer20001510753min256.99365.74480.73546.62636.56μm7min480.73μm44(R20.9799)SBRQin[16]SBR5min3Fig.3Sludgecharacteristicsvariationduringexperiment62627EPS2.4(EPS)Liu[17]EPS[18−20]55EPS(PS)(PN)7min4Fig.4Relationshipbetweenaveragesludgeparticlesizeandsettlingtime5Fig.5VariationsofEPSduringthesludgegranulationPS(VSS)(140.9819.54)mg/g(310.7950.86)mg/gPN(44.081.55)mg/g(73.663.56)mg/gPNPS(0.3160.241)Liu[17]Adav[21]EPSα-β-β-β-α-EPS3(1)SBR(2)SBRSBR5min(3)MLVSSMLSS(4)[1]MishmaK,NakamuraM.Self-immobilizationofaerobicactivatedsludge:Apilotoftheaerobicupflowsludgeblanketprocessinmunicipalsewagetreatment[J].WaterScienceandTechnology,1991,23(4/5/6):981−990.[2]SchwarzenbechN,ErleyR,MCSwainBS,etal.Treatmentofmaltingwastewaterinagranularsludgesequencingbatchreactor(SBR)[J].ActaHydrochimicaetHydrobiologica,2004,32(1):16−24.[3]XUH,TayJH,FooSK,etal.Removalofdissolvedcopper(Cu)andzinc(Zn)byaerobicgranularsludge[J].WaterScienceandTechnology,2004,50(9):155−160.()442628[4]TayJH,JiangHL,TaySTL.Highratebiodegradationofphenolbyaerobicallygrownmicrobialgranules[J].JournalofEnvironmentalEngineering,2004,130(12):1415−1423.[5]DuqueAF,BessaVS,CarvalhoMF,etal.2-Fluorophenoldegradationbyaerobicgranularsludgeinasequencingbatchreactor[J].WaterResearch,2011,45(20):6745−6752.[6]AdavSS,LeeDJ,ShowKY,etal.Aerobicgranularsludge:recentadvances[J].BiotechnologyAdvances,2008,26(5):411−423.[7]MorgenrothE,SherdenT,vanLoosdrechtMCM,etal.Aerobicgranularsludgeinasequencingbatchreactor[J].WaterResearch,2007,31(12):3191−3194.[8]APHA.StandardMethodsfortheexaminationofwaterandwastewater[S].20thed.WashingtonDC:AmericanPublicHealthAssociation/AmericaWaterWorksAssociation/WaterEnvironmentFederation,1998.[9]LIUYu,WANGZhiwu,QINLei,etal.Selectionpressuredrivenaerobicgranulationinasequencingbatchreactor[J].AppliedMicrobiologyandBiotechnology,2005,67(1):26−32.[10]LIUYu,TayJH.Theessentialroleofhydrodynamicshearforceintheformationofbiofilmandgranularsludge[J].WaterResearch,2002,36(7):1653−1665.[11]AdavSS,LeeDJ,TayJH.Activityandstructureofstoredaerobicgranules[J].EnvironmentalTechnology,2007,28(11):1227−1235.[12]MoyYP,TayJH,TohSK,etal.Highorganicloadinginfluencesthephysica