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39320183ENVIＲONMENTALSCIENCEVol．39No．3Mar．2018*300350SBＲSMP、．SMPCOD71～85mg·L－1SMP、EPS．SMP3×10354.8%～71.7%100×103SMP9.3%～14.5%．SMP4、、．GC-MSSMP、、39.0%、14.9%、11.7%7.6%．SMP32.0%．X172X703A0250-3301201803-1325-08DOI10．13227/j．hjkx．2017070462017-07-072017-09-06516082791993～E-mailSunflowerfordan@163．com*E-mailhuangwenli@nankai．edu．cnCharacterizationCompositionofSolubleMicrobialProductsinanAerobicGranularSludgeSystemYANGDanLIUDong-fangDULi-qiongHUANGWen-li*CollegeofEnvironmentalScienceandEngineeringNankaiUniversityTianjin300350ChinaAbstractUsingflocculentactivatedsludgeasseedsludgetocultivateaerobicgranularsludgeinaSBＲthemainobjectiveofthisstudywasfocusedontheaccumulationrelativemolecularmassdistributionandcompositionofsolublemicrobialproductsSMPinanaerobicgranularsludgeAGSsystem．SMPwerepredominant71-85mg·L－1intheeffluentoftheAGSsystem．TheformationofSMPwasrelatedtosubstrateutilizationbiomassdecayandEPShydrolysis．ArelativemolecularmassdistributionanalysisindicatedthatthemajorityofSMPaccountingforabout54.8%-71.7%hadMr＜3×103whereastheMr＞100×103formedasmallfractionconstitutingonly9.3%-14.5%．Three-dimensionalexcitationemissionmatrixfluorescencespectra3D-EEMidentifiedfourpeaksinSMPbelongingtoaromaticprotein-liketryptophanprotein-likehumicacid-likeandfulvicacid-likesubstances．Gaschromatography-massspectrometryGC-MSanalysisrevealedthatesters39.0%shortchainalkanes14.9%alkenes11.7%andalcohols7.6%werethemaincompoundsinSMP．Mostnotablybis2-ethylhexylphthalateasonekindofesteraccountedfor32%oftheidentifiedSMP．KeywordssolublemicrobialproductsSMPaerobicgranularsludgeAGSrelativemolecularmassdistributionbis2-ethylhexylphthalategaschromatography-massspectrometry12．、、34．5．SMP67．SMP、、SMPCOD89SMP1011．MBＲSMPXie11SMPLiang12SMPMBＲSＲTs．SMPLi1326-39SMPEPS．SMP、、SMP．11.11.1.1SBＲ100cm6cm2.8L2L．30cm1．、、、、513L·min－1．1.1.22．1.1.31Fig．1DiagramoftheSBＲpH7.8～8.414．3．1Table1Operationtimeforonecycle/d/min/min/min/min/min1～7228185～20015～527～120228202322Table2CharacteristicsofseedsludgeMLSS/mg·L－1MLVSS/mg·L－1MLVSS/MLSSSV30/%SVI/mL·g－1662232840.492842.33Table3ComponentsofsyntheticwastewaterCODNH4ClNKH2PO4PCaCl2CaMgCl2·6H2OMgFeSO4·7H2OFe/mg·L－160050105551.2SMP12000r·min－115min0.45μmSMPSMPCOD．SMP3×103、10×103、50×103100×103BedfordMAUSA．COD1516．Aquino17SMP．SMP=COD－1.07×1.3MLSS、MLVSS、SVI30、NH+4-NCOD．EPS20mL8060min4℃12000r·min－120min623130.22μmEPS18COD．SDPTOPCX40CANONHPLC．AgilentC184.6mm×250mm5μmpH2.101mL·min－120μL210nm．1.4SMP．HitachiF-7000．200～400nm5nm200～550nm5nm．10nm1200nm·min－1．1.5GC-MSSMPGC-MSSMP．、5mL0.5mL4℃．GC-MS7890B-5977AGC-MSAgilentHP-5MS30m×0.25mm×0.25μm．30℃3min8℃·min－1280℃25min．280℃2μLHe1.0mL·min－12.5min．NIST11．LNationalInstituteofStandardsandTechnologyGaithersburgMDUSAhttp//．nist．gov/srd/mslist．htm．22.12．90d．ab40dc60dd90d2Fig．2Imagesofsludgeinthegranulationprocess420min3minMLSS6.6g·L－14.5g·L－1MLSS．90d0.92mm10.7g·L－1．2.2SMP3CODNH4-N86%99%．7231394120Table4Characteristicsofgranulesinthereactorsduring120daysofoperation/dMLSS/g·L－1MLVSS/MLSSSVI30/mL·g－1/mm06.60.49420.11304.50.73320.24606.60.93270.519010.70.89110.9212010.30.91151.13COD78～90mg·L－1SMP71～85mg·L－1．SMPCOD．3SBＲCODNH+4-NSMPFig．3EffluentconcentrationandremovalefficienciesofCODNH+4-NandSMPintheSBＲSMP、EPS、CODSMP4．SMPSMP．SMP．SMP．EPSEPSSMP1920．Ni21boundEPSUAPboundEPSBAP．SMPEPS．COD90min42mg·L－1CODEPSSMP．4SMP、EPS、CODFig．4VariationofSMPEPSacetateandCODintheSBＲinatypicalbatchexperiment2.3SMPSMP40d100dSMP5．SMP3×103SMP54.8%～71.7%100×103SMP9.3%～14.5%．SMP3×103～10×10310×103～50×1036.5%～8.6%8.6%～17.2%．50×103～100×103SMPSMP3.7%～5.0%．Liang12MBＲSMP．40d100dSMP．40d100dSMP3×10352.8mg·L－166.7mg·L－1．82313SMP＞100×103、50×103～100×103、10×103～50×1033×103～10×10313.9mg·L－18.7mg·L－14.8mg·L－13.5mg·L－116.5mg·L－18.0mg·L－18.3mg·L－16SMPFig．63D-EEMfluorescencespectraofSMPondifferentoperationdays6.1mg·L－1．40d100dSMPSMPSＲT．440d100dSＲT10d21d．SＲTSMPSMP2223．2.4SMPSMP40d100dSMP．6540d100dSMP4PeakAEx/Em=230/340～345nmPeakBEx/Em=270～280/340～350nmPeakCEx/Em=340～345/420～425nmPeakDEx/Em=245/380～395nm．Chen244PeakA、PeakB、PeakCPeakD．40d100dSMP4．40d100dSMP4．100dSMP40dSMP2.3．5SMPFig．5TheMrdistributionsandfractionofSMPintheeffluentondifferentoperationdays2.5-GC-MSSMPSMPSMPGC-MSSMP80%4661%．79231395Ｒ1Ｒ2SMPTable5DetailedfluorescencespectraparametersofSMPssamplesonday40and100/dEx/Em/nmA230/340～34521440B270～280/340～350167C340～345/420～425147.4D245/380～395165.4A230/340184.8100B280/350158.1C345/425125.5D245/380150.6、、、．39%14.9%、11.7%7.6%．Zhang25GC-MSMBＲSMP、、38%、17%、15%15%．SMPMＲＲ．7SMPGC-MSFig．7GC-MSanalysisfortheSMPcompositionintheeffluentoftheAGSsystem6、、12345--3--2-SMP、、32.03%、5.60%、7.21%2.93%．．、26．．2728SMP．6GC-MSSMPTable6CompoundsdetectedbyGC-MSinSMPintheeffluentintheSBＲ/min/%/%2.9733--2-86.1C5H10O5.4384.232.9972--2--106.1C5H11Cl2.695.983.2223--2-86.1C5H10O2.9395.173.299100.1C7H165.686.714.552NN-115.1C6H13NO1.2182.324.914-3-100.1C6H12O1.0693.456.5282--3--140.2C10H201.4989.336.84912345--138.1C10H187.2195.117.27612345--140.2C10H203.287.967.864244--126.1C9H181.4280.67.9231--3--140.2C10H202.5290.616.04224a88-206.2C15H261.1482.3729.088346.2C18H35BrO3.7680.4731.21