好氧颗粒污泥亚硝化工艺的启动与运行特性研究杨洋

,＊,,(,　100084):,,(30～35℃),NH+4-N2.5～3.0kg(m3·d),90%;100mgLCOD;(20℃).:;;:X703.1　:A　:0250-3301(2007)11-2462-05:2006-10-23;:2007-04-29:(863)(2002AA601190):(1979～),,,,E-mail:[e-mail]＊,E-mail:[e-mail]-upandOperationCharacteristicsofAerobicGranularShort-CutNitrificationProcessYANGYang,ZUOJian-e,BUDe-hua,GUXia-sheng(DepartmentofEnvironmentalScienceandEngineering,TsinghuaUniversity,Beijing100084,China)Abstract:Inalab-scaleaeratedupflowsludgebed(AUSB)reactorinoculatedwiththemixtureofanaerobicgranularsludgeandaerobicsludge,usingsyntheticammonia-richwastewaterasinfluent,aerobicshort-cutnitrificationgranuleswerecultivated.Afterthat,theshort-cutnitrificationreactorcouldbeoperatedstablywithveryhighefficiencyundermesophiliccondition(30～35℃),andtheinfluentloadingrate(NH+4-N)couldreach2.5～3.0kg(m3·d).Theammoniaremovalefficiencyandtheshort-cutnitrificationratio(NO-2NO-x)wereabove90%respectively.SomeorganicCOD(about100mgL)existedintheinfluenthadnoobviouseffectsontheoperationoftheshort-cutnitrificationprocess.Theshort-cutnitrificationreactorcouldalsobeoperatedstablywithveryhighefficiencyunderambienttemperature(about20℃).Keywords:short-cutnitrificationprocess;aerobicgranularsludge;AUSBreactor　　SHARON(singlereactorforhighactivityammoniaremovalovernitrite)[1]、-[2],.,2:①(30～35℃)(ammoniaoxidationbacteria,AOB)(nitriteoxidationbacteria,NOB),30～35℃,1d,NOB[1,3];②AOBNOB,(1mgL),,AOB,NOB[4～7].,1d,,;,,,;,2,.,,、,.1　1.1　1,(aeratedupflowsludgebed,AUSB),3.05L,1.60L,1.45L.,、,..,2811200711　　　　　　ENVIRONMENTALSCIENCEVol.28,No.11Nov.,2007DOI:10.13227/j.hjkx.2007.11.006,328～351d20℃,30～35℃.1.;2.;3.AUSB;4.;5.;6.;7.;8.;9.1　Fig.1　Experimentalapparatusandprocessschemechart　1.2　,,　　UASB.,AUSB(VSS,)2.0gL,VSS0.4gL,VSS1.6gL.,NH4HCO3、10∶1KH2PO4,L1mL,[8]:15gLNa2EDTA·2H2O、0.43gLZnSO4·7H2O、0.24gLCoCl2·6H2O、0.99gLMnCl2·4H2O、0.25gLCuSO4·5H2O、0.22gLNaMoO4·2H2O、0.19gLNiCl2·6H2O、0.08gLNa2SeO30.02gLH3BO4.1.3　:[9];:DX-100;COD:TL-1ACOD;pH:ThermoOrion828pH;:Thermo810A;SSVSS:[9].2　2.1　AUSB140d,40d,NH+4-N0.5kg(m3·d);100d,NH+4-N2.5kg(m3·d).AUSB2.2　AUSBFig.2　PerformanceofAUSBreactorduringstart-upperiod　　　2,40d,300mgL,,100mgL,,.35d,pH6.77.5,,,40d,5mgL,.41～60d,550mgL,NH+4-N1.0kg(m3·d),,,95%,,,,20～200mgL,,.61～80d,246311:,.81～140d,550mgL,NH+4-N2.5kg(m3·d),,,,120mgL,,300mgL,(5mgL),,AUSB.,,AUSB.、,,,,,,、,,.[10][11].AUSB,3.3,ASUB,,1.0mm.,.,,ASUB,.3　Fig.3　Photoofaerobicshort-cutnitrificationgranularsludge　2.2　140d,AUSB,,,(),,,,.,2:141～171d,,AUSB;209～309d,,.,AUSBNH+4-N2.5～3.0kg(m3·d).AOB,AOB.,,AUSB15d,.,141～171d,pH7.5,DO3mgL5mgL.AUSB4.4　141～171dAUSBFig.4　PerformanceofAUSBreactorduringthedaysof141～171　4,AUSB600mgL,,90%.,2464　　　　　　28,400mgL,20mgL.,AUSB,90%.,,,,209～309d,.,AUSB,100mgL.209～309d,AUSB5.5,AUSB600～700mgL,:249d,50mgL;250～285d,50～150mgL;286d,50mgL.,,,,,VSS0.013g·(L·d)-1,,VSS0.089g·(L·d)-1.209～285d,,,,250～285dDO3mgL1mgL.,,,AOB,,,75%.AOB,,286d,,DO3mgL,,,.5,,,CODDO,95%.,,AOB,,.2.3　5　209～309dAUSBFig.5　PerformanceofAUSBreactorduringthedaysof209～309　　　,30～35℃,,,,,,.,.,328～351d,(20℃),AUSB6.328d,AUSB35℃20℃,pH7.5,3mgL.6,,35℃20℃,6　AUSBFig.6　PerformanceofAUSBreactorunderambienttemperature　,.,330d350mgL,NH+4-N1.5246511:kg(m3·d).,,338d,700mgL,NH+4-N3.0kg(m3·d).6,,50mgL,90%,50mgL.30～35℃,AUSB,20mgL50mgL,,,,,.2.4　“”,,2,AOBNOB2[12,13],.,,.,,“”(、).,,、pH:,;pH,;,.、pH,,、,,,[14].,AOBNOB,,,,.3　(1)AUSB,,30～35℃、pH7.5、DO3mgL15d,,NH+4-N2.5～3.0kg(m3·d),90%.(2)(30～35℃)、DO3mgL、pH7.5,100mgLCOD,NH+4-N2.5～3.0kg(m3·d),90%.(3)20℃,10d,20℃,pH7.5、DO3mgL、NH+4-N2.5kg(m3·d),90%.:[1]HellingaC,SchellenAAJC,MulderJW,etal.TheSharonprocess:aninnovativemethodfornitrogenremovalfromammoniumrichwastewater[J].WatSciTech,1998,37(9):135～142.[2]vanDongenU,JettenMSM,vanLoosdrechtMCM.TheSharon-anammoxprocessforthetreatmentofammoniumrichwastewater[J].WatSciTech,2001,44(1):153～160.[3]HellingaC,vanLoosdrechtMCM,HeijnenJJ.Modelbaseddesignedofanovelprocessforammoniaremovalfromconcentratedflows[J].MathematicalandComputerModellingofDynamicalSystems,1999,5(4):351～371.[4]LaanbroekHJ,BodelierPLE,GerardsS.OxygenconsumptionkineticsofNitrosomonaseuropaeaandNitrobacterhamburgensisgrowninmixedcontinuousculturesatdifferentoxygenconcentration[J].ArchMicrobiol,1994,161(2):156～162.[5]GarridoJ,vanBenthumWAJ,vanLoosdrechtMCM,etal.Influenceofdissolvedoxygenconcentrationonnitriteaccumulationinabiofilmairliftsuspensionreator[J].BiotechnolBioeng,1997,53(2):168～178.[6]BernetN,PengDC,DelgenesJP,etal.Nitrificationatlowoxygenconcentrationinbiofilmreator[J].JofEnvirEngin,2001,127(3):266～271.[7]WangJL,YangN.Partialnitrificationunderlimiteddissolvedoxygenconditions[J].ProcessBiochem,2004,39(10):1223～1229.[8],,.[M].().:,2004.90.[9].[M].().:,2002.276～279.[10].[M].().:,1998.56.[11],,.[J].,2002,21(3):317～321.[12],.[J].,2004,10(4):521～525.[13]ArpDJ,BottomleyPJ.Nitrifiers:Morethan100yearsfromisolationtogenomesequences[J].Microbe,2006,1(5):229～234.[14].[J].,2000,26(11):21～23.2466　　　　　　28