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12112121215009221500928。E-mailfeiyuechandler@163．com513083673313112032014-09-182014-10-21PSAGS、、。AGS“”EPSGranulanALEPS、。AdvancesintheStudyonGel-FormingPropertyofExopolysaccharidesIsolatedfromAerobicGranularSludgeQianFeiyue12WangYan1WangJianfang12ShenYaoliang121CollegeofEnvironmentalScienceandEngineeringSuzhouUniversityofScienceandTechnologySuzhou2150092KeyLabofEnvironmentalScienceandEngineeringofJiangsuProvinceSuzhou215009AbstractAsthekeystructuralcomponentsinaerobicgranularsludgeAGSgel-formingexopolysaccharidesPSwerecharacterizedbystrongstabilitylowbiodegradabilityandhighcrosslinkingetc．IntheviewofthewideapplicationprospectofAGStechnologyandofthenewbreakthroughin“polysaccharides”mechanismbothcontentrangeandspatialdistributionofmainextracellularpolymericsubstancesEPSwereintroducedfirstly．Inparticularanup-to-datereviewonthechemicalcompositionproposedsourceandstructuralroleoftypicalgel-formingPSisolatedfromAGSincludinggranulanandalginate-likeALEwasprovidedinthispaper．Atlastthegenomeanalysesoffunctionmicrobialandmoleculardynamicssimulationsweresuggestedtobeundertakeninthefuture．KeywordsExopolysaccharidesAerobicgranularsludgeGel-formingpropertyCrosslinkactionStructuralstabilityEPS、PNPSEPS70%～90%TOC1。EPS、、PNPS。AGS、、2。PN3～6。PNGibbs578。PS、910。PS“”“”。Seviour11-·023·2015784http//．hxtb．orgDOI:10.14159/j.cnki.0441-3776.2015.04.005、。、EPS。AGSEPSPS、。1AGSEPS1.1EPS1EPS。EPSDNA、G6PDH812～14。1EPSTab．1TheEPScompositionsofflocsandgranulesludgesreportedinliteratures*EPSEPS/mg·gVSS－1PS/%PN/%DNA/%PS/PNCSTＲNH4-NEDTA137.59.1%63.4%1.1%0.1413CSTＲ-NaOH164.924.6%33.1%0.3%0.7415CSTＲ/－NaOH200～23028%～34%55%—0.52～0.6116SBＲ--NaOH732.314.9%73.4%0.1%0.2017SBＲ483.430.2%47.2%＜0.1%0.6418SBＲNH4-N285.16.0%84.9%1.5%0.078SBＲNO3-N-NaOH120～170≤10%≥90%—＜0.14SBＲ113～13630%65%～70%—0.5～0.719SBＲEDTA88.762.2%26.5%—2.0～2.520SBＲNH4-N-NaOH————1.5～2.09*SBＲCSTＲ。EPStightly-boundTBEPS813。EPSPS/PN0.2～0.94512～16。、、PS51921。Ni20COD170mg/LSBＲSequencingBatchＲeactorActivatedSludgeProcess300d0.2～0.8mmEPSPS/PN2.0。Wu9COD/NH4-N1～215～20dPS、。1.2EPSEPS。1CLSMAGSEPS1。EPS。EPS·123·http//．hxtb．org2015784PS1019。100μm2022。AGSPS2324。1CLSM20Fig．1CLSMimagesofthecryosectionsofaerobicgranulesludgeafterstainingbyvariousfluorescenceprobes20aAGS、、、、α-β-2bAGS2AGS2.1PSPS2526。1-、PN23NMＲ、MALDI-TOF-MSFTIＲ。PS2527。Granulan82、GranulanpKa9.02829。Granulan0.33%25。MDSβ-D-β-GlcN。Ca2+Granulanβ-D-β-Galβ-D-β-GlcA30。pHpH6.0～8.5GranulanG'G″1025。2Granulan2829Fig．2IllustrationofthestructureofrepeatunitinGranulangel-formingpolysaccharide2829ALEα-Gβ-M、3α-GGblocksCa2+ALEpKa4.527。Lin26ALEALEgranulesALEflocs2ALEgranulesGGblocks69%ALEflocs35%。2%CaCl2ALEgranules16～18h2～5mm、90%ALEflocs。3ALEab27Fig．3IllustrationofthestructureofapolymersandbrepeatunitsinAlginate-likegel-formingpolysaccharide27GranulanALE、EPS31。-AGSPS。28℃pH7.0XanthomonascampestrisGranulanXanthan32。Mucins·223·2015784http//．hxtb．orgGelatinePS31。Seviour33GPCEPSpHAGS105～106DaPS。2.2PSEPS312334。。Seviour29GranulanGAOsCompetibacterphosphatis。NMＲGAOs25%PSGranulan。PAOsAccumulibacterphosphatisGranulan。GAOsCompetibacterGranulan。PseudomonasAzotobacterALE35。ALE。2.3PSEPSloosely-boundLBEPSAGSTB-EPSPSAGS3637。。EPS、59。Ni20PS/PNAGSPS。EPS、、31。AGS“”。PS、3438。Gao39AGS8PS。Adav18β-β-PSAcinetobacterSp．I8BacillussphaericusI5I8。GranulanAGSPS。Adav40β-PS、α-PS。Xiong41AGS1mg/mLK2d。PS。PNPSAGS。10～50mg/LCa2+Mg2+AGS4243。、AGS4445。46Ca2+AGSZeta。Ca2+、EPS-Ca-EPSMg2+EPS47。Li48Mg2+10mg/LAGS32d18dPS1。3·323·http//．hxtb．org2015784、、。GranulanALEPSAGS。PS、EPS。Ca2+Mg2+。、PS。PS、。、AGS。1GPShengHQYuXYLi．Biotechnol．Adv．2010286882～894．2SSAdavDJLeeKYShowetal．Biotechnol．Adv．2008265411～423．3LLZhangXXFengNWZhuetal．EnzymeMicrob．Technol．2007415551～557．4YJLiuDDSun．Proc．Biochem．2011464987～992．5LZhuMLLvXDaietal．Bioresour．Technol．2012107346～54．6．2014872697～2670．7．20123351614～1619．8CPellicer-NacherCDomingo-FelezAGizemMutluetal．WaterＲes．201347155564～5574．9LWuCYPengYZPengetal．J．Hazard．Mater．2012242234～241．10SSAdavDJLee．J．Hazard．Mater．20081541～31120～1126．11TSeviourMPijuanTNicholsonetal．Biotechnol．Bioeng．200910251483～1493．12SSAdavDJLee．J．Hazard．Mater．20081541～31120～1126．13ZWLiangWHLiSYYangetal．Chemosphere2010815626～632．14PDAbzacFBordasEvanHullebuschetal．Appl．Microbiol．Biotechnol．20108551589～1599．15HLiuHPFang．J．Biotechnol．2002953249～256．16．200554121624～1629．17SSAdavDJLee．J．TaiwanIns．Chem．Eng．2011423645～651．18SSAdavDJLeeJYLai．J．Hazard．Mater．20101741～3424～428．19DWGaoLLiuHLiangetal．J．Hazard．Mater．20111861320～327．20BJNiWMXieSGLiuetal．WaterＲes．2009433751～761．21YHKongYQLiuJHTayetal．J．Hazard．Mater．2009455379～383．22SDWeberWLudwigKHSchleiferetal．Appl．Environ．Microbiol．200773196233～6240．23MYChenDJLeeJHTay．Appl．Environ．Microbiol．20077361463～1469．24MVerawatyMPijuanZYuanetal．WaterＲes．2012463761～771．25TWSeviourMPijuanTNicholsonetal．WaterＲes．200943184469～4478．26YMLinPKSharmaMCMvanLoosdrecht．WaterＲes．201347157～65．27YMLinMdeKreukMCMvanLoosdrechtetal．WaterＲes．201044113355～3364．28TSeviourLKLambertMPijuanetal．Environ．Sci．Technol．201044238964～8970．29TSeviourLKLambertMPijuanetal．Appl．Microbiol．Biotechnol．20119261297～1305．30TSeviourAKMaldeSK