好氧颗粒污泥中丝状微生物生长研究

Seediscussions,stats,andauthorprofilesforthispublicationat:[Filamentousovergrowthinaerobicgranules]Article  in  Huanjingkexue=Huanjingkexue/[bianji,ZhongguokexueyuanhuanjingkexueweiyuanhuiHuanjingkexuebianjiweiyuanhui.]·March2008Source:PubMedCITATIONS8READS855authors,including:Someoftheauthorsofthispublicationarealsoworkingontheserelatedprojects:OrganohalideRespiration:NewFindingsinMetabolicMechanismsandBioremediationApplicationsViewprojectEnvironmentalAnaerobicMicroorganismsViewprojectShanquan(Alan)WANGSunYat-SenUniversity33PUBLICATIONS   317CITATIONS   SEEPROFILEAllcontentfollowingthispagewasuploadedbyShanquan(Alan)WANGon21November2015.Theuserhasrequestedenhancementofthedownloadedfile.,,,,*(,100875):(SSBR)2,.,.R1R3,R1R3,R1R3,R2.,R1=14R2=12R3=20.,R1Eikelboom0092Nocardiaspp.,R2Fungispp.Nocardiaspp.,R3S.natansH.hydrossis,,,.,.:;;SSBR:X7031:A:02503301(2008)03069607:20070320;:20070413:(50478093);:(1982~),,,,Email:[e-mail]*,Email:[e-mail]inAerobicGranulesWANGShanquan,KONGYunhua,YUANYuan,DONGXiaolei,ZHUJianrong(SchoolofEnvironment,BeijingNormalUniversity,Beijing100875,China)Abstract:Filamentousovergrowthhasbeenobservedafteraerobicgranulationinshakingsequencingbatchreactors(SSBR).Thefactorsaffectingthefilamentousovergrowthinaerobicgranulesanditspossiblecontrolmethodswereinvestigated.Itwasfoundthatboththeinfluentandsludgeinoculationlargelyinfluencedthefilamentousovergrowthinaerobicgranules.Withthesameactivatedsludgeinoculation,thefilamentousovergrowthinR3treatingfreshwastewaterwasmuchmoreseverethanthatinR1treatingsalinewastewater.ThefilamentousovergrowthinR2wastheleastamongthethreereactors,whichwasinoculatedanaerobicgranulesandtreatedsalinewastewater.Whenthefilamentousovergrowthhappenedattheouteraerobicgranules,referringthedegreeoffilamentousovergrowthachievedR1=14,R2=12andR3=20,respectively.FilamentousidentificationshowedthatthefilamentousmicroorganismsinR1mainlycomposedofEikelboom0092andNocardiaspp.,Fungispp.andNocardiaspp.inR2,S.natansandH.hydrossisinR3.TheidentifiedfilamentousmicroorganismslikelyoccurredinconditionswithlongSRT,lowdissolvedoxygenandbiodegradablesubstrates.However,duetothefactthatfilamentousovergrowthinaerobicgranulesinitiatedintheinnerpartofthegranulesandactedasframeworkswithrodshapeorcoccoidbacteriaentrappingintointhegranulationperiod,variousstrategieseffectivelyindealingwithconventionalbulkingproblemsincludingshortingSRT,changingorganicloadingrateandenhancingaerationetal,couldnoteffectivelyinhibitthefilamentousgrowthinaerobicgranules.Thefilamentousovergrowthwasultimatelycontrolledthroughchangingthesubstratefromsyntheticglucoserichwastewatertononbiodegradablewastewater.Keywords:aerobicgranules;filamentousovergrowth;SSBR,,.[1][2][3,4].,[5].[6~10],,.,,,.29320083ENVIRONMENTALSCIENCEVol.29,No.3Mar.,2008.1112,,COD900mgL-1.NaCl,10gL-1NaCl,2gL-1NaCl,3gL-1NaCl5gL-1NaCl,[11].13gL-1NaCl,,Vc,COD1200mgL-135gL-1.(SSBR)[12],1.3SSBR,R1R2R3.R1R2,.R3,.05L.:12h,5min,710min,1min,4min.,251,,160rmin.1Fig.1Schematicrepresentationofthebioreactor12Olympus.[13],[14].,,:,Dd,=Dd..GCMSFinniganTrace2000,Vc:GC,50,15min,29010min,N2,1mLmin;MS,EI,70eV,200,250,mz35~1000,1L.133:P(1~15d)P(16~40d)P(41~70d).1.1.1Table1ConfigurationofthereactorsR1R2R3PgL-14810638105105125P120~150105~130150~250P110105115221SSBR,,.R1R31,[1,10]SBR15~30d.R2,,R2PP.R2[15].2P.6973:ABCR1R2R3,DEFR1R2R32Fig.2Microscopicobservationofseedsludge(A,B,C)granularsludge(D,E,F)inR1,R2andR3,:,R1R3,,SSBR(1),,;.P,R1R3,,03~05mm,R1R32.(10gL-1NaCl)R145mh-1R3375mh-1,R1.R1R3,R1105,R312.R2R1R3,.R22,R23:,,2,R23,514mh-1.,:R2,Beun[1],;R1R3,3,,SSBRSBR,,SSBRSBR,,SSBR.22,.SSBRSSBR.P,,4,698293SSBRFig.3MicroscopicobservationoftheaerobicgranulationinSSBRwithactivatedsludgeinoculation.P,,,R1=14R2=12R3=20.,[14],5~7.4Fig.4FilamentousovergrowthwithinaerobicgranulationR1[5(A)]Eikelboom0092[5(B)]Nocardiaspp.[5(C)]2,Eikelboom0092.Eikelboom0092,Nocardiaspp.[16],R148d.R2[6(A)]Fungispp.[6(B)]Nocardiaspp.[6(C)].,Fungispp.25pH6.5~7.0,.,[9,10].R3[7(A)]S.natans[7(B)]H.hydrossis[7(C)].S.natansH.hydrossis[16].:R16993:(A);(B)Eikelboom0092;(C)Nocardiaspp.5R1Fig.5FilamentousmicroorganismsofaerobicgranulesinR1(A);(B)Fungispp.;(C)Nocardiaspp.6R2Fig.6FilamentousmicroorganismsofaerobicgranulesinR2(A);(B)S.natans;(C)H.hydrossis7R3Fig.7FilamentousmicroorganismsofaerobicgranulesinR3R3,.,R2.,,.2330d,.,,,.,[01kg(kgd)-1[17]];,6mgL-1;;,.,[5],,:R15d,R2CODR2,R3Vc,160rmin200rmin.R1R2,.R3,20.,PVc.,,,Vc[11].P,R105~2mm(8A)R215~3mm(8B)R315~25mm(8C),R111R2105R3115.P:,700298PFig.8MorphologyofgranularsludgeinreactorsatPVc,,.,VcGCMS,9Vc.GCMSVc,Vc10C,.,,,Vc.9VcGCMSFig.9AnalysisoforganiccompoundsinVcwastewaterbyGSMS3(1).R1R3,R1R3,R1R3,R2.(2),7013:R1=14R2=12R3=20.:R1Eikelboom0092Nocardiaspp.,R2Fungispp.Nocardiaspp.,R3S.natansH.hydrossis.,,.(3),,,.:[1]BeunJJ,HendriksA,vanLoosdrechtMCM,etal.Aerobicgranulationinasequencingbatchreactor[J].WaterRes,1999,33:22832290.[2],,,.[J].,2004,20(11):4648.[3],,,.SBR[J].,2004,24(3):355359