好氧条件下CANON工艺的启动研究付昆明

CANON1,1,2,1,1,1,1(1.,,　100124;2.,　150090):DOCANON(completelyautotrophicnitrogenremovalovernitrite),,CANON.,35℃±1℃,pH7.39～8.01.60d,(NO-2-NNO-x-N)98%,;160d,,210d,TN1.22kg(m3·d),TN70%,CANON.,CANON,CANON:①;②,8,,8.61.:;ANAMMOX;CANON;:X703.1　:A　:0250-3301(2009)06-1689-06:2008-07-10;:2008-09-29:(50878003);(08UWQA08);(KZ200610005001):(1981～),,,,E-mail:[e-mail]rt-upUnderAerobicConditionsFUKun-ming1,ZHANGJie1,2,CAOXiang-sheng1,LIDong1,MENGXue-zheng1,ZHUZhao-liang1(1.KeyLaboratoryofBeijingWaterQualityScienceandWaterEnvironmentRecoveryEngineering,CollegeofArchitectureandCivilEngineering,BeijingUniversityofTechnology,Beijing100124,China;2.SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090,China)Abstract:Thestart-upofCANONprocesswasstudiedtorevealadaptabilityandhowtocondensetimeofstart-upwithalab-scaleexperiment.Duringstart-upstage,temperaturewascontrolledat35℃±1℃,pHwas7.39-8.01andfreeammoniumwas2.89-12.37mg·L-1.Partialnitritationwasfirstbuiltupin60thdayandkeptsteady,nitriteaccumulationrate(NO-2-NNO-x-N)wasupto98%.Inthe160thday,thereactorstartedtoshoweffectsofanaerobicammoniumoxidation,andinthe210thday,CANONprocesswasstarted-upunderaerobicconditionssuccessfully,andtotalnitrogenremovalloadwas1.22kg(m3·d)and70%removalrateonaveragewasattained.Whenusingammoniumwastewaterwithoutorganiccarbonsourcesasinfluent,therearetwosignstoindicatetheCANONstart-up:first,nitrogengaswasproducedincarriers;theotheronewastheratiooftotalnitrogenlossandincreasednitratekeepsteady,andinthisstudyaverageratiowas8.61comparedtotheoreticalvalue8.Keywords:partialnitritation;ANAMMOX;CANON;sponge　　2090Mulder[1](anaerobicammoniumoxidation,ANAMMOX),、、[2],.ANAMMOX[3,4],,ANAMMOX,,ANAMMOX.,ANAMMOX2,SHARON+ANAMMOX,[5];ANAMMOX,CANON(completelyautotrophicnitrogenremovalovernitrite).Sliekers[6]ANAMMOX,SBRCANON.CANONANAMMOX,ANAMMOX,,,ANAMMOX.,(1)[7].1NH3+0.85O20.11NO-3+0.44N2+0.14H++1.43H2O(1)　　,CANON[6～8],CANON,ANAMMOX,ANAMMOXCANON[7],ANAMMOX[9],30620096　　　　　　ENVIRONMENTALSCIENCEVol.30,No.6Jun.,2009DOI:10.13227/j.hjkx.2009.06.039CANON,,.CANON,ANAMMOX,CANON.1　1.1　1,,3.85L,,,2cm×2cm×2cm,1g·cm-3..,XMT-102,35℃±1℃.pHHI931700pH7.39～8.01.1.;2.;3.;4.;5.;6.;7.;8.;9.;10.pH;11.1　Fig.1　Experimentalapparatusandprocessschemechart　1.2　SBR.24h,,,75%.,18L·d-1.NH4Cl、NaHCO3、NaClK2HPO4.,,,,,NO-2-N,NO-3-NNO-3-N.1.1　mg·L-1Table1　Waterqualityofinfluentmg·L-1NH+4-NNO-2-NNO-3-NPO3-4-PNaClpH120～3000～103～5104007.5～8.51.3　NH+4-N:[10],NO-2-N:N-(1-)-[10],NO-3-N:[11],pH:pHTestr30pH;:HI2400;MLSS:711.2　2.1　ANAMMOX,ANAMMOXNO-2-NNH+4-N1.32∶1[12],,NH+4-N,NH+4-NNO-2-N,.,50%～60%NH+4-N,ANAMMOXNO-2-NNH+4-N.,,1g·cm-3,.,,,1g·cm-3,.,NH+4-N、NO-2-NNO-3-N2～4.　　26d,12.5～15.6m3(m3·h).,.SBR.0～9dNH+4-N120～140mg·L-1,NH+4-N,9d,NH+4-N65%;NH+4-N180mg·L-1,NH+4-N30%,,27d,NH+4-N50%～60%.NH+4-N280mg·L-1,,,2.,1690　　　　　　302　NH+4-NFig.2　NH+4-Ninpartialnitritationstart-upstage　3　NO-2-NFig.3　NO-2-Ninpartialnitritationstart-upstage　4　NO-3-NFig.4　NO-3-Ninpartialnitritationstart-upstage　NH+4-NNH+4-N,NH+4-N,0.3kg(m3·d)0.68kg(m3·d).NO-2-N25d,NO-2-N60mg·L-1,35d,NO-2-N140mg·L-1,,NO-2-N100～150mg·L-1,3;NO-3-N35d,NO-3-N,5mg·L-1,NO-3-N,12mg·L-1,NO-3-NNO-3-N1.87mg·L-1,4,,(NO-2-NNO-x-N),98%.60d,,.,,2.,Ford[13]NO-2-N30～36℃,,(ammoniumoxidationbacteria,AOB)(nitriteoxidationbacteria,NOB).35℃±1℃,NO-2-N,AOBNOB.SHARON,,NOB[14].(freeammonium,FA),Anthonisen[15],FA(2).AnthonisenFAAOBNOB,NOBAOB.FANOBAOB0.1～1.0mg·L-110～150mg·L-1.FA,NOBAOB.,pHpH7.39～8.01,FA2.89～12.37mg·L-1,NOBAOB,,.[15,16]FA,FA.FA=1714×[NH+4-N]×10pHKbKw+10pH(2),Kw,Kb.,AOBNOB,FANOB,AOB16916:CANON,AOBNOB,.,,,DO,,DO3～4mg·L-1,DO(AOB0.2～0.4mg·L-1,NOB1.2～1.5mg·L-1[17]).2.2　ANAMMOXANAMMOX,NH+4-N、NO-2-N、NO-3-NTN5.5　ANAMMOXNH+4-N、NO-2-N、NO-3-NTNFig.5　NH+4-N,NO-2-N,NO-3-NandTNinANAMMOXstart-upstage　　　,,DOANAMMOX(Strous[9]0.5%～2.0%,ANAMMOX,100mg·L-1,ANAMMOX[18]),120d,18.7m3(m3·h)6.2m3(m3·h),NO-2-N163.73mg·L-170～80mg·L-1.,,ANAMMOX[19],TN,,ANAMMOX,AOB[20].160d,,,,,,,1g·cm-3,.,NO-2-N,ΔNO-3-NΔTN.180d,NO-2-N20mg·L-1,ΔNO-3-N11.25mg·L-1,ΔTN92.61mg·L-1,(1),(ANAMMOXANAMMOX).NH+4-N,210d,NH+4-N380mg·L-1,24.9m3(m3·h),ΔNO-3-N36.58mg·L-1,ΔTN262mg·L-1,TN80%,70%.TN1.22kg(m3·d),Furukawa[8]0.48kg(m3·d).1692　　　　　　30,CANON,TN.,,CANON160d,,CANON,210d.160d,2,,;ΔNO-3-NΔTN.160d,ΔNO-3-NΔTN,ΔTNΔNO-3-N8.61,(1)ΔTNΔNO-3-N=0.44×20.11=8.NH+4-NCANON,ΔTNΔNO-3-N,CANON.2.3　ANAMMOXANAMMOX,ΔTN,(、),,2L,MLSS1250mg·L-1,35℃±1℃,DO0.2mg·L-1,NO-2-NNH+4-N,6h,1h,NH+4-N、NO-2-N、NO-3-NpH,6.6　ANAMMOXFig.6　ProoftestofANAMMOXactivity　6,5h,NH+4-NNO-2-N,NO-3-N,pH,,,NH+4-NNO-2-N1∶1N2,NO-2,,NO-3-N,NH+4-N、NO-2-NNO-3-N1∶1.32∶0.26[12],1∶1.36∶0.22,.NO-2-N,,pH.,ANAMMOX.3　　　(1)CANON,35℃±1℃,pH7.39～8.01,210d,CANON,1.22kg(m3·d),.(2)ANAMMOX,pH,98%.(3),NH+4-NCANON,CANON:①;②,,8.61.:[1]　MulderA,vanGraafAA,RobertsonLA,etal.Anaerobicammoniumoxidationdiscoveredinadenitrifyingfluidizedbedreactor[J].FemsMicrobiolEcol,1995,16(3):177-184.[2]　,.[J].,1997,19(4):25-28.[3]　StrousM,FuerstJA,KramerEH,etal.Missinglithotrophidentifiedasnewplanctomycete.[J].Nature,1999,400(6743):446-449.[4]　vanGraafAA,Muld