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21520029JournalofWuxiUniversityofLightIndustryVol.21No.5Sep.2002:1009-038X(2002)05-0515-04:2002-05-02;:2002-06-26.:(1965-),,,,.,,(,214036):,NO2-,N,,30h200mg/L99%,NO2-.pH710,pH:pH8,pH,,,3.9g/L,pH62.5%.:;;;:Q939:AMixedCultureofDenitrifyingBacteriaforAerobicSimultaneousNitrification2DenitrificationDUGuo2cheng,GENGJin2ju,CHENJian(KeyLabofIndustrialBiotechnology,MinistryofEducation,SouthernYangtzeUniversity,Wuxi214036,China)Abstract:NH4+wasconvertedtoNO2-underaerobicconditionsbythescreenedbacteria,theintermediateNO2-wasthenreducedtoN2byaerobicdenitrifyingbacteria,andnoNO2-wasaccumulated.200mg/LofNH4+canberemovedwithin30hwitharemovalrateof99%.TheappropriatepHforthegrowthofmixedbacteriawas710.pHcontrolpolicyforthehighdensitymixedculturein5Lfermentorwasproposed.BycontrollingpHvaluelowerthan8intheearlystageandwithoutpHcontrollingafterwards,cellgrowthperiodwasshortenedandNH4+removalratecouldbeimproved,drycellweightreached3.9g/L,increased62.5%higherthanthatundernaturalpHconditions.Keywords:denitrifyingbacteria;nitrification2denitrification;aerobicdenitrifying;mixedculture,.,,.,,2080,.Pseudomonasspp,Al2转载[13],(NO2-)(NO3-)(N2).,,,:(1),NO2-NO3-,;(2),OH-,pH;(3),,.,.pH.,,pH,pH,.,5LpH,pH,pH,.11.1:(Aerobicdenitrify2ingBacteria),.(g/L):NaHCO31.75,CH3COONa0.854,(NH4)2SO41.0,NaCl1.0,FeSO40.4,K2HPO41.0,MgSO40.25.NaNO2NaNO3.1.2:,50mL500mL,30,200r/min.:5L(KF25L)3L,10%,30,400r/min,1L/(Lmin),pH.1.3:[4].:5mL11000r/min10min,,0.1mol/LHCl,105,(DCW).:2N,N2[5].:[4].22.1,(NH4+N)(NO2-N(NO3-N).NO2-NNO3-N,NH4+N,NO2-NNO3-N,.,,,,.2.2NO2-NO3-,,,,,,,.,,.NH4+NO2-NO3-,NH4+N(r)NO2-NNO3-N1(a,b,cd).(a)61521中国科技论文在线(b)NH4+N(c)NO2-N1NH4+NO2-NO3-Fig.1ParameterchangeswiththeadditionofNH4+NO2-andNO3-1(d),NO3-,NO3-,NO3-;NH4+N,NO3-.2(a),.1(c),NO2-,NO2-,12hNO2-0.05g/L0.003g/L,()1.7g/L,NO2-,[6].NO2-NH4+,NH4+N,NO2-,30hNO2-100%.NO2-N,,,[7].NO2-,NH4+N,NO2-NNO3-N,.NH4+NO2-NO3-,(),(Aerobicshortcutsimultaneousnitrificationdenitrification),NH4+NNO2-N,,NO2-NN2.2:2Fig.2Sketchofbio2denitrificationprocess,,,[8]:(1)25%,;(2)40%,;(3);(4),NO2-NO3-63%.7155:中国科技论文在线=7(pH),pH=8(pH),pH7pH8pH.3(ab),pH,pH,.(a)(b)35LpHFig.3TimecoursesofDCWandtheremovalrateofammonia2nitrogenunderdifferentpHconditionsin5Lfermentor3(a)3(b),,,pH.pH,pH(4).pH8,,20h,pH10h.3.9g/L,62.5%,.pH7,,pH,pHpH,,.,pH,pH78,.,pH8,.45LpHFig.4TimecourseofpHchangesin5Lfermentor3(),,NH4+NNO2-N,,NO2-NN2.pH8,pH,,,3.9g/L,pH62.5%.:[1]ROBERTSONLA,VANNIELEWJ,TORREMANSRAM.Simultaneousnitrificationanddenitrificationinaerobicchemo2statculturesofThiosphaerapantotropha[J].Applied&EnvironmentalMicrobiology,1988,54(1):2812-2818.(532)81521中国科技论文在线(g/L)SBA/u2/%172.039.3111.4100.3266.849.1116.0100.0:SBA,100,,NH4+,SBA.3SBA[4],NH4+,;91%,80%83%,SBA120125,L2.,:108110,120125.:[1].[M].:,1995.570-587.[2].[M].:,1999.427.[3]SMITHTJ,MATHIASLJ.Newmethacrylatederivativesbasedonpyroglutamicacid[J].PolymerPreprints,2000,41(2):1254-1255.[4].[A].[C].:,2001.79-83.(:)(518)[2]OTTES,SCHALKJ,KUENENJG.HydroxylamineoxidationandsubsequentnitrousoxideproductionbytheheterotrophicammoniaoxidizerAlcaligenesfaecalis[J].AppliedMicrobiology&Biotechnology,1999,51(2):255-261.[3]GUPTAAB.Thiosphaerapantotropha:Asulphurbacteriumcapableofsimultaneousheterotrophicnitrificationandaerobicdenitrification[J].Enzyme&MicrobialTechnology,1997,21(8):589-595.[4].()[M].:,1989.[5],,.N,N.[J],1992,11(3):75-77.[6]YOOH,AHNKH,LEEHJ,etal.Nitrogenremovalfromsyntheticwastewaterbysimultaneousnitrificationanddenitrifica2tion(SND)vianitriteinanintermittently2aeratedreactor[J].WaterResearch,1999,33(1):145-154.[7]MULLEREB,STOUTHAMERAH,VANVERSEVELDHW.SimultaneousNH3oxidationandN2productionatreducedO2tensionsbysewagesludgesubculturedwithchemolithotrophicmedium[J].Biodegradation,1995,6(4):339-349.[8]JETTENMSM.Towardsamoresustainablemunicipalwastewatertreatmentsystem[J].WaterScienceTechnology,1997,35(9):171-180.(:)23521中国科技论文在线