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2003,23(6)587~591ChinaEnvironmentalScience,,(,100084),3.1LEGSB.,EGSBpH6.0,400mgCaCO3/L,5.3kgCOD/(m3⋅d).,EGSBpH5.8~6.0,COD3000mg/L,5.2kgCOD/(m3⋅d)29d,COD89.2%,264.4mgCaCO3/L,56.9%..X522A10006923(2003)06058705Studiesoncultivationofacid-tolerantmethanogenicgranularsludgeusingriversediments.LINGXue-feng,ZUOJian-e,GUXia-sheng(DepartmentofEnvironmentalScienceandEngineering,TsinghuaUniversity,Beijing100084,China).ChinaEnvironmentalScience,2003,23(6)587~591AbstractStudiesonthecultivationofacid-tolerantmethanogenicgranularsludgeina3.1Lexpandedgranularsludgebed(EGSB)reactorusingriversedimentsasseedsludgewasconducted.Inthereactor,acid-tolerantgranularsludgewithgoodsettlingabilityandmethanogenicactivitywascultivatedundertheconditionsofpH6.0,effluentalkalinitylowerthan400mgCaCO3/L,loadingrate5.3kgCOD/(m3⋅d).Thereafter,undertheconditionofpH5.8~6.0,influentCOD3000mg/Landloadingrate5.2kgCOD/(m3⋅d),thereactoroperatedstablyfor29dayswiththeaverageCODremovalrateof89.2%,effluenttotalalkalinityof264.4mgCaCO3/Landthemethanecontentofabout56.9%inbiogas.Investigatedunderscanningelectronicmicroscope,manyMethanothrixsoehngeniiwerefoundaggregatedinclusterintheinnerareasofgranularsludge.KeywordsgranularsludgeacidicconditionriversedimentsmethanogenesispH7.0~7.2[1].,pH,,.[2,3],.,,,(pH6.0),,[4,5].,,[6].,,.11.11,EGSB3.1L.35±1.,,CODCr:N:P=200:5:1,20030217(50008009);863(2002AA601190);(9700032)*58823NH4ClKH2PO4;150mg/L[Fe2(SO4)33.57mg/L;ZnSO4⋅7H2O0.44mg/L;NiSO4⋅6H2O0.89mg/L;Na2MoO4⋅2H2O0.50mg/L;CoCl2⋅6H2O0.81mg/L;MgSO4⋅7H2O5.13mg/L].pH1mol/LNaOHpH.1Fig.1Experimentalapparatusandprocessflowchart1.2.3.EGSB4.5.6.7.8.9.10.11.pH12.pH13.14.1mol/LNaOH1.2COD:TL-1ACOD;pH:Orion828;pH:MiltonRoypH,:[7],pH3.8;:LML-2();:,1490GC-TCD(),:2.5m×2mm,:OV-101,:70,:100,:100;,VSS/SS:[7];(ORP):[7];[8];:.22.14.8gVSS/L,pH6.0.7d,.,4.2gVSS/L,3.0m/h.,,pH.2,3,64d,pH7.046.7;,pH7.01650mgCaCO3/LpH6.7820mg/L;0.1kgCOD/(m3⋅d)8kgCOD/(m3⋅d);COD95%,,.,,.2pHFig.2pHvaluesandeffluentalkalinitiesofthereactorduringstart-up2.2,97d,.1d65d,4,5.4,5,pH7.4,1.3kgCOD/(m3⋅d)1dpH6.810d,2.6kgCOD/(m3⋅d),COD234567891011121314558985%;pH6.4,4d,pH6.232d,,2.6kgCOD/(m3⋅d)6.9kgCOD/(m3⋅d),COD90%.112d,pH6.029d,5.5~6.0kgCOD/(m3⋅d),COD90%.141d,pH5.8,pH29d,5.2~5.5kgCOD/(m3⋅d),COD89.2%.3CODFig.3OLRsandCODremovalefficienciesofthereactorduringstart-up465~169dpHFig.4pHvaluesandeffluentalkalinitiesofthereactorfromday65to1694,,,200~400mgCaCO3/L,213mgCaCO3/L.(155~169d),pH5.8~6.0.,.Ferguson[9],pH7.0,pH6.650%.565~169dCODFig.5OLRsandCODremovalefficienciesofthereactor,ORPpH.pH6.8,ORP363mV;pH5.8,ORP260mV.6CH4CO2H2.pH,CH4pH6.870.8%pH5.856.9%;CO2;H2,0.6%.pH5.8,1gCOD302.2mL,350mL,EGSBCH4CO2.665~169dFig.6Gasproductionanditscompositionofthereactorfromday65to169CH4CO2H2590232.32.3.1,64d,pH(6.7~7.0),VSS/SS,4.2gVSS/L10.7gVSS/L,VSS/SS0.090.64.95d,,8.0gVSS/L,VSS/SS,0.45.105d,VSS/SS,0.82;,2.7gVSS/L,.102~169d,,0.93gVSS/d.(pH5.8~6.2)(Yobs)0.044gVSS/(gCOD⋅d),[10].,pH(5.8~6.2),,.pH.2.3.2119d,1.pH6.0,5.3kgCOD/(m3⋅d),COD90.3%,3.0m/h.,.,0.5~1.0mm.pH5.8,,57.0%.pH5.8,,1.4mm51.8m/h;0.9~1.4mm44.5m/h;0.4~0.9mm27.9m/h.,pH5.8,(SMA)531.2mLCH4/(gVSS⋅d),1.76gCOD/(gVSS⋅d).2.3.3(SEM),7.7Fig.7SEMphotosoftheseedsludgeandacid-tolerantgranularsludgeabcdef7a,0.06~0.20mm,.7b~7f142d,pH5.8abcdef5591SEM.7b~7f,(7b),,(7c)..,.,(7c,7d),.,(Methanothrixsoehngenii),1.5~2.8ìm,(7e).(7f),(Methanosarcina)..33.13.1LEGSB,pH,pH6.0,400mgCaCO3/L,5.3kgCOD/(m3⋅d),.3.2,pH5.8,COD3000mg/L,5.2kgCOD/(m3⋅d),COD89.2%,264.4mgCaCO3/L,56.9%.EGSBpH5.8~6.289d,pH.3.3SEM,,.[1]DuarteAC,AndersonGK.Inhibitionmodelinginanaerobicdigestion[J].Wat.Sci.Technol.,1982,14:749763.[2]WilliamsRT.Methaneproductioninminnesotapeatlands[J].Appl.Environ.Micro.,1984,6:12661271.[3]GoodwinS.EcophysiologicaladaptationsofanaerobicbacteriatolowpH:Analysisofanaerobicdigestioninacidicbogsediments[J].Appl.Environ.Micro.,1987,1:5764.[4]JainSR,MattiassonB.AcclimatizationofmethanogenicconsortiaforlowpHbiomethanationprocess[J].BiotechnologyLetter,1998,20(8):771775.[5]BrummelerET,HulshoffPLW,DolfingJ,etal.Methano-genesisinanupflowanaerobicsludgeblanketteactoratpH6onanacetate-propionatemixture[J].Appl.Environ.Micro.,1985,6:14721477.[6]SavantDV,ShoucheYS,PrakashS.Methanobrevibacteracididuranssp.nov.,anovelmethanogenfromasouranaerobicdigester[J].InternationalJournalofSystematicandEvolutionaryMicrobiology,2002,52:10811087.[7].[M].3.:,1997.[8],,,.EGSB[J].,2002,20(4):37.[9]FergusonJF,BrianJE,BenjaminMM.Neutralizationinanaerobictreatmentofanacidicwaste[J].WaterRes.,1984,18(5):573580.[10].[M].4.:,2000.589.(1977),,,,.4.