荷花不同部位浸出液对3种淡水藻类生长的影响孟繁丽

36[1-3]。、[4-7]。[8-9]。[10]。Fitzgerald[11]。30、[12-14]———。。。《》2012-08-3[e-mail]*[e-mail]。EnvironmentalScience&Technology36620136Vol.36No.6Jun.2013Nelumbonucifera孟繁丽1，2，何连生2*，席北斗2，李定龙1，张进保2，舒俭民21.5102802.100012、。3。EC50EC504.21g/LEC505.35g/L、9.92g/L、EC509.52g/L、7.28g/L、6.90g/L。X172Adoi10.3969/j.issn.1003-6504.2013.06.0041003-6504(2013)06-0016-05EffectofLeachatefromDifferentPartsofNelumbonuciferaontheGrowthofThreeFreshwaterAlgaeMENGFan-li12HELian-sheng2*XIBei-dou2LIDing-long1ZHANGJin-bao2SHUJian-min21.ChangzhouUniversityChangzhou510280China2.ChineseResearchAcademyofEnvironmentalScienceBeijing100012ChinaAbstractEffectsofleachatefromdifferentpartsofNelumbonuciferaonthegrowthofMicrocystisaeruginosaChlorellapyrenoidesScenedesmusquadricandawerediscussedtoprovideabasisfortheallelopathicsubstancesfromlotustoreducelakeeutrophication.ResultsshowedthattheleachatefromdifferentpartsofNelumbonuciferasignificantlyinhibitedthegrowthofthreealgae.ThelotusleafhadabetterinhibitoryeffectthanNelumbonuciferastem.TheleachatefromdifferentpartsofNelumbonuciferahaddifferenteffectsofhalf-concentrationEC50ofdifferentalgae.EC50ofMicrocystisaeruginosaChlorellapyrenoidesScenedesmusquadricandawas4.215.359.92g/Lrespectively.TheleachatefromNelumbonuciferastemonMicrocystisaeruginosaChlorellapyrenoidesScenedesmusquadricandawas9.527.286.90g/Lrespectively.KeywordsNelumbonuciferafreshwateralgaeleachate611.1实验材料、M11。25℃8000Lux12h：12h3~5。48h。48h。。1.2实验方法0、5、10、15、20、25mL5mL500mL200mL。g/L0、5、10、15、20、25g/L。、1、2、3、4、5。1.3测试方法1mL0.1mLNikon-E200。2。24h6d。1IR=1-NN0×100%11IRNN0。EC50。6dEC50144h144hEC50[15]。22.1荷花茎浸出液对铜绿微囊藻、蛋白核小球藻及四尾栅藻生长的影响2.1.11。122.5×108/mL2.2×108/mL22。2~5316.37%、45.46%、43.57%、53.45%。25g/L。31。2.1.22。。1。2~5。54570.50%。2.1.33。1。233459.38%、68.13%。4514481.25%、81.57%。545。3620g/L。2.2荷花叶浸出液对铜绿微囊藻、蛋白和小球藻及四尾栅藻生长影响2.2.14。1232d45。20g/L667.27%25g/L665.45%。2.2.255g/L。21~3d3。61.31%、50.82%、61.64%、69.84%、56.90%。2.2.3610mg/L。132。234555.63%、70%、79.38%、86.25%。。25g/L79.38%。2.3荷花不同部分浸出液对藻细胞增长率影响1。5g/L2.97。3。25g/L10g/L2.03。10g/L10g/L。。。。2.4半浓度效应计算结果61Table1Theeffectoftheleachateoflotusfloweronthegrowthofthreealgae。/g·L-1/%/%/%068.30±5.00102.32±1.80137.54±8.00536.95±2.40-34.35±0.8013.46±2.7010-34.35±1.9013.78±1.10-28.00±3.2015-51.16±0.50-9.08±0.08-26.09±1.8020-59.54±3.20-11.31±1.10-50.01±2.0025-69.60±1.90-10.51±0.90-60.25±3.10534.35±0.0259.16±2.6012.25±0.9010-49.6±0.89-10.64±1.30-14.40±0.2015-50.08±1.10-10.37±0.50-65.34±1.1020-63.16±2.00-20.27±2.10-70.32±2.1025-55.13±2.10-17.57±1.90-77.62±0.6073EC506.90g/LEC509.52、7.30g/LEC50EC50EC504.21g/LEC509.92g/LEC505.35g/L。35g/L5g/L3。1.081.10。5g/L。20~70cm。、、。[16-18]。[19]。。、。。、。。。。413。5g/L20g/L64.29%、69.84%25g/L79.38%-63.16±2.00%、-20.27±2.10%、-77.62±0.60%。2、、EC504.21、5.35、9.92g/L、EC509.52、7.23、6.90g/L。EC50。36（上接第15页）[9].[J].2009316159-163.LvRui-hengLiuYongYuHai-qunetal.SoilfertilityofdifferentforesttypesinthemountainousareaofBeijing[J].JournalofBeijingForestryUniversity2009316159-163.inChinese[10].[J].2006548-51.LiChao-shengYangXiao-huiYuChun-tangetal.Het－erogeneityofsoilcharacteristicaboutdifferentvegetationtypesinnorthofOrdosPlateau[J].JournalofSoilandWaterConservation2006548-51.inChinese[11]WangYanLiuRu-haiGaoHui-wangetal.DegenerationmechanismresearchofSuaedaHeteropterawetlandoftheShuangtaiziEstuaryNationalNatureReserveinChina[J].ProcediaEnvironmentalSciences201021157-1162.[12].[J].200223277-81.BaiJun-hongDengWeiZhangYu-xiaetal.Spacialdis－tributioncharacteristicsofsoilorganicmatterandnitrogeninthenaturalfloodplainwetland[J].EnvironmentalScience200223277-81.inChinese[13]WilliamJMJarnesGG.Wetlands[M].NewYorkVanNos-trandReinholdCompanyInc198689-l25.[14].[J].200928102092-2097.DingQiu-yiBaiJun-hongGaoHai-fengetal.Soilnu－trientcontentsinYellowRiverDeltawetlandswithdifferentplantcommunities[J].JournalofAgro-environmentScience200928102092-2097.inChinese[15].N、P、K[J].200928101977-1985.YangYong-xingLiuChang-eYangYang.CharacteristicsofNPandKcyclinginScirpusmariqueterwetlandecosys－tematJiuduanshashoalofYangtzeRiverestuary[J].ChineseJournalofEcology200928101977-1985.inChinese[][1]BackerLCMcNeelSVBarberTetal.Recreationalex－posuretomicrocystinsduringalgalbloomsintwoCalifornialakes[J].Toxicon201055909-921.[2].[M].,20111-15.[3]PaerlHWScottJT.Throwingfuelonthealgalgrowthbymacrophytesandmacrophyteextractedbioactivecompounds[J].WateredScienceandTechnology201034227-235.[4]FalconerIR.Anoverviewofproblemscausedbytoxicblue-greenalgaecyanobaceriaindrinkingandrecreationalwater[J].EnvironmentToxicology,1999145-12.[5]HeislerJClibertPMBurkholderJMetal.Eutrophicationandharmfulalgalbloomsascientificconsensus[J].HarmfulAlgal200883-13.[6]RychteckyPZnachorP.Spatialheterogeneityandseasonalsuccessionofphytoplanktonalongthelongitudinalgradientinaneutrophicreservoir[J].Hydrobiologia2011663175-186.[7].[J].20041752-61.[8]JingWangJunyingZhuShaopingLiu.Generationofreac－tivespeciesincyanobacteriaandgreenalgaeinducedbyal－lelochemicalsofsubmergedmacrophytes[J].Chemosphere2011885977-982.[9].[J].