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:025322468(2004)0320394206:X70115:A1,2,2(11,315016;21,310029):,3,3,4,52(TCS).,g015mg,TCS,30%.60d,COD,,SOUR.,60d,,,.,TCS,.:;;;3,3,4,52(TCS);ReductionofexcesssludgeproductionbychemicaluncouplerinactivatedsludgeprocessYEFenxia1,CHENYingxu2,FENGXiaoshan2(1.DepartmentofChemicalEngineering,NingboCollege,Ningbo315016;2.DepartmentofEnvironmentalEngineering,ZhejiangUniversity,Hangzhou310029)Abstract:3,3,4,52tetrachlorosalicylanilide(TCS),ametabolicuncouplerwasaddedtocompletelymixedactivatedsludgeprocess.ThecontinuoustestshaveconfirmedthatTCSwasaneffectivechemicaluncouplerinreducingtheexcesssludgeproductionby30%whenitsspecif2icconcentrationwashigherthan015mgg-1.ItwasalsorevealedthatCODremovalefficiencyandsludgesettleabilityfromthetreatedandcontrolsamplerswerecomparableandnotsignificantlydifferent,buteffluentnitrogenconcentrationwasaffectedadverselybythepresenceofTCSduringthe60doperation.SuchasludgegrowthreductionwasassociatedwiththeenhancementofmicrobialactivitiesintermsoftheSOUR.ThefilamentousbacteriaweremoreinthesludgefromsystemwithadditionofTCSthanthoseinthecontrolreactorafter60doperation.FewerprotozoaandmetazoangrowinthesludgefromthesystemwiththeadditionofTCSandtheiractivitiesweremuchlowerthancontrolsludge.TheseresultssuggestedthatitmightbepossibletoapplyTCSinactivatedsludgesystemstoreducetheexcesssludgeproductionbutitmightinfluencethepopulationinsludge.Keywords:activatedsludgeprocess;excesssludgereduction;protonphore;3,3,4,52tetrachlorosalicylanilide(TCS);uncoupler:2003204211;:2003211215:(50201080):(1968),,(),yefenxia@hotmail.com,,.,,,..TeijsselTempest,,(energyspilling)(uncoupling),[13].,24320045ACTASCIENTIAECIRCUMSTANTIAEVol.24,No.3May,2004,.,,[412].,,.,3,3,4,52(TCS),,TCSTCS,.1111,1Fig.1Diagramofthetestapparatus1.1315L,318L,516L.,,,,,,.112.3,3,4,52(TCS)(9910%)Acros.1132,.,,1.1Table1ComponentsandconcentrationsofsyntheticmunicipalwastewaterP(mgL-1)P(mgL-1)268MgSO466200CaCl26132KH2PO448.868(NH4)2SO41128FeSO40.3NaHCO380MnSO46::CODCr=600mgL-1,BOD5=280mgL-1,=110mgL-1,=60mgL-11142,,1,TCS,.70d.(HRT)4163d,(FPM)0133kg(kgd)-1.10dTCS(014gL-1)3,2mL,214mg.,60d1TCS(15mg).,TCS,TCS(),.26mgL-1.(MLSS)3000mgL-1,5933:,MLSS3000mgL-1.25.114:SVI;CODCr;MLSS(VSS);2.(SOUR):DO,300mL,.20min,MLVSS,SOUR,mg(mgd)-1,,.2211TCS260d2.2,22Fig.2Excesssludgeproductioninthe60dcontinuousoperation360dCODFig.3CODremovalefficienciesoftwosystemsduringthe60dcontinuousoperation.,2147gd-1,TCS,1173gd-1,3010%.TCS,60dTCS,,TCS,,..212TCS2COD3.,TCSCOD.27d,,COD,,COD.TCS.2,4,62[4]2,42[6][7,8]TCS[9,10].45.,TCS(NH+42N)(TN).Low,,,,,.,69324460dNH+42NFig.4EffluentNH+42Nconcentrationsofthetworeactorsduringthe60dcontinuousoperation560dTNFig.5EffluentTNconcentrationsofthetworeactorsduringthe60dcontinuousoperation660dSVIFig.6SVIvalueofthetwosystemsduringthe602daycontinuousoperation760dSOURFig.7SOURvalueofthetwosystemsduringthe60dcontinuousoperation.,TCS,TCS,,,.,2,.60d,SVI,SVI82181,TCSSVI95122,(6),Chen[9,10].213TCSSOUR7.7,TCS,2129mg(mgd)-12193mg(mgd)-1,28167%.May2hewStephenson[6]2,42,.SOUR,,TCS,.,.2,7933:,.TCS,,(8).8(a,b:;c,d:TCS)Fig.8Thescanningelectronmicroscopicimageofthesludge(aandb:sludgefromcontrolsystem;candd:sludgefromsystemwithadditionofTCS)60d,2.,.TCS,.,,(8).TCS,,.Low,,[7,8].,TCS.3,TCS,COD..SOUR,,.60d,,,,TCS,,..,,.89324:[1]CookGM,RussellJB.EnergyspillingreactionsofStreptococcusbovisandresistanceofitsmembranetoprotonconductance[J].ApplEnvironMicrobiol,1994,60(6):19421948[2]TempestW,NiejsselM.Physicologicalandenergeticaspectsofbacterialmetaboliteoverproduction[J].FEMSMicrobiolLett,1992,100(1):169176[3]RussellJB,CookGM.Energeticsofbacterialgrowth:balanceofanabolicandcatabolicreactions[J].MicrobiolReviews,1995,59(1):4862[4]StrandSE,HaremGN,StenselHD.Activatedsludgeyieldreductionusingchemicaluncouplers[J].WatEnvironRes,1999,71(4):454458[5]RiveranevaresJA,WymanJF,VonmindenDL,etal.2,62ditert2butyl242nitrophenol(DBNP)apotentiallypowerfuluncouplerofoxidativephosphorylation[J].EnvironToxicolChem,1995,14(1):251256[6]MayhewM,StephensonT.Biomassyieldreduction:isbiochemicalmanipulationpossiblewithoutaffectingactivitysludgeprocessef2ficiency?[J].WatSciTechnol,1998,38(829):137144[7]LowEW,ChaseHA,MilnerMG,etal.Uncouplingofmetabolismtoreducebiomassproductionintheactivatedsludgeprocess[J].WatRes,2000,34(12):32043212[8]LowEWandChaseHA.Theuseofchemicaluncouplersforreducingbiomassproductionduringbiodegradation[J].WatSciTech2nol,1998,37(425):399402[9]ChenGH,MoHK,LiuY.Utilizationofametabolicuncoupler,3,3,4,52tetrachlorosalicylanilide(TCS)toreducesludgegrowthinactivatedsludgeculture[J].WatRes,2002,36(8):20772083[10]ChenGH,MoHK,SabyS,etal.Minimizationofactivatedsludgeproductionbychemicallystimulatedenergyspilling[J].WatSciTechnol,2002,42(12):189200[11]OkeyRW,StenselHD.Uncouplersandactivatedsludge2theimpactonsynthesisandrespiration[J].ToxicolEnvironChem,1993,40(1):235254[12]LowEW,ChaseHA.Reducingproductionofexcessbiomassduringwastewatertreatment[J].WatRes,1999,33(5):111911329933: