常用载体及通用测序引物

VectorPrimer(F)Primer(R)抗性载体大小(bp)EZ-TM13-47RV-MAmp+2984LZRS-RfALZRS-FLZRS-R11100p3xFLAGCMV14CMV30(834bp)CMV24(1151bp)6310p3XFlag-myc-CMV-24CMV-F(852bp)/PCEP-F(896bp)R(hGHpolyA)(1217bp)4752pAAV-MCSF(β-globin)(1192bp)R(hGHpolyA)(1521bp)4650pAc5.1/V5-HisAAc5-Profor(2525bp)BGH(2768bp)/EBVrev(2844bp)Amp+5375pAc5.1/V5-HisBAc5-Profor(2525bp)BGH(2772bp)/EBVrev(2848bp)Amp+5379pAc5.1/V5-HisCAc5-Profor(2525bp)BGH(2764bp)/EBVrev(2840bp)Amp+5371pAcGP67_APH-Profor(4016bp)pAcGP67rv(4329bp)Amp+9761pAcGP67_BPH-Profor(4016bp)pAcGP67rv(4329bp)Amp+9761pAcGP67_CPH-Profor(4016bp)pAcGP67rv(4329bp)Amp+9761pACTT7(1061bp)T3(1420bp)Amp+5566pACT25'AD(同GAL4AD)(5134bp)3'AD(4939bp)/pGAL4-Adrv(4964bp)(pGAL4-Adrv离MCS少于Amp+811720bp，优先用3'AD)PACYCDuet-1(MCSⅠ)DuetUP1(3949bp)DuetDOWN1(209bp)Chl+4008PACYCDuet-1(MCSⅡ)DuetUP2(189bp)T7ter(468bp)Chl+4008pAdTrackpEGFP-N-3’(同EGFP-Nrev)(1294bp)CMV-F(1433bp)Amp+/Kan+8111pAdTrack-CMVpEGFP-N-3’(同EGFP-Nrev)(1272bp)EBVrev(1982bp)Kan+9010PAO8155'AOX3'AOX7700pAS2-15'BD(同GAL4BD)(5894bp)3'BD(6112bp)/pGAL4-BDrv(6046bp)(优先用3'BD)Amp+8392pB42ADpB42ADF(787bp)pB42ADR(924bp)Amp+6449PBABE-IRES-EgfppLXSN-F(同pLXSNfw)(1027bp)/PBABE5'(1063bp)/pBMN5'(1416bp)无反向引物5944pBabe-puropBabe5'(1318bp)pBabe3'(1429bp)5169pBACPAK8BAC1(1181bp)/PH-Profor(1174bp)/BaculoVfor(1211bp)BAC2(1374bp)/BaculoVrev(1375bp)Amp+5538pBAD/HisApBADfw(211bp)/Xpressfor(375bp)(优先用pBADfw)pBADrv(514bp)4102pBBR1MCSM13F(3161bp)/T7(3188bp)/M13-47(3135bp)M13R(3390bp)/T3(3352bp)/RV-M(3409bp)Amp+4707pBI12135S(5735bp)无14758pBK-CMVT7(975bp)/M13F(951bp)T3(1159bp)/M13R(1196bp)Amp+4518pBluescriptIIKS(-)M13F(599bp)/T7(626bp)T3(792bp)/M13R(829bp)2961pBluescriptIIKS(+)M13F(599bp)/T7(626bp)T3(792bp)/M13R(829bp)2961pBluescriptIISK(-)M13F(599bp)/T7(626bp)T3(792bp)/M13R(829bp)2961pBlueScriptIISK(+)M13F(599bp)/T7(626bp)T3(792bp)/M13R(829bp)2961pBluescriptKS(-)M13F(602bp)/T7(626bp)T3(790bp)/M13R(828bp)2958pBluescriptKS(+)M13F(602bp)/T7(626bp)T3(790bp)/M13R(828bp)2958pBluescriptSK(-)M13F(602bp)/T7(626bp)T3(790bp)/M13R(828bp)2958pBluescriptSK(+)M13F(602bp)/T7(626bp)T3(790bp)/M13R(828bp)2958pBSN-TT7T3Amp+pBudCE4.1(MCSI)CMV-Profor(552)/T7(638)c-mycrev(742bp)/EBVrev(842bp)4595pBudCE4.2(MCSⅡ)PEF-F(2999)BGH(3235)4595pBV220PBV220F(3592bp)PBV220R(93bp)3667pCAMBIA1300P1(11472bp)P2(2728bp)11566pCAMBIA1301P1(10935bp)P2(11149bp)11849pCAMBIA1304P1(11454bp)P2(11668bp)12361pCAMBIA2300M13F(8418bp)/M13R(-48)(8313bp)M13F(-47)(8461bp)/M13R(8349bp)8742pCANTAB_5EpCANTAB5-S1(2265bp)/pCANTAB5-R1(2222bp)pCANTAB5-S6(2471bp)/pCANTAB5-R2(2504bp)4517pCAT3-enhancerRVP3(4215bp)无Amp+4273pCDFDuetT7(214bp)T7ter(467bp)3781pCDH-CMV-MCS-EF1-PuroCMV-F(2164bp)/PCEP-F(2208bp)/pLNCX-F(2210bp)无7384pcDNA1.1CMV-F(2076bp)/T7(2170bp)无4000pcDNA2.1M13F/T7M13R/SP63013pcDNA3.0pcDNA3fw(841bp)/CMV-Profor(777bp)/T7(863bp)SP6(1016bp)/BGH(1053bp)5446pcDNA3.1(-)T7(863bp)/CMV-Profor(777bp)/pcDNA3fw(841bp)BGH(1039bp)5428pcDNA3.1(+)T7(863bp)/CMV-Profor(777bp)/pcDNA3fw(841bp)BGH(1039bp)5428pcDNA3.1/HisAT7(863bp)/CMV-Profor(777bp)BGH(1122bp)Amp+5514pcDNA3.1/HiT7(863bp)/CMV-Profor(7BGH(1122bp)Amp+5514sB77bp)pcDNA3.1/HisCT7(863bp)/CMV-Profor(777bp)BGH(1122bp)Amp+5514pcDNA3.1/myc-HisAT7(863bp)/CMV-Profor(777bp)BGH(1099bp)/c-mycrev(1020bp)Amp+5494pcDNA3.1/myc-HisBT7(863bp)/CMV-Profor(777bp)BGH(1099bp)/c-mycrev(1020bp)Amp+5494pcDNA3.1/myc-HisCT7(863bp)/CMV-Profor(777bp)BGH(1099bp)/c-mycrev(1020bp)Amp+5494pcDNA3.1/V5-HisAT7(863bp)/CMV-Profor(777bp)BGH(1108bp)/V5rev(1035bp)Amp+5502pcDNA3.1/V5-HisBT7(863bp)/CMV-Profor(777bp)BGH(1108bp)/V5rev(1035bp)Amp+5502pcDNA3.1/V5-HisCT7(863bp)/CMV-Profor(777bp)BGH(1108bp)/V5rev(1035bp)Amp+5502pcDNA3.1/Zeo(-)T7(863bp)/CMV-F(769bp)BGH(1038bp)Amp+5014pcDNA3.1/Zeo(+)T7(863bp)/CMV-F(769bp)BGH(1039bp)Amp+5015pcDNA3.3/TOPOTACMV-F(584bp)/PCEP-F(628bp)/pLNCX-F(630bp)无Amp+5407pcDNA4/HisMaxAXpressfor(1119bp)BGH(1282bp)Amp+5258pcDNA4/HisMaxBXpressfor(1119bp)BGH(1282bp)Amp+5258pcDNA4/HisXpressfor(1119bp)BGH(1282bp)Amp+5258MaxCpcDNA4/HisMax-TOPOXpressfor(1119bp)BGH(1302bp)Amp+5274pcDNA4/HisMax-TOPO/lacZXpressfor(1119bp)BGH(4482bp)Amp+8354pcDNA4/myc-HisACMV-Profor(777bp)/T7(863bp)BGH(1103bp)/c-mycrev(1024bp)Amp+5079pcDNA4/myc-HisBCMV-Profor(777bp)/T7(863bp)BGH(1103bp)/c-mycrev(1024bp)Amp+5079pcDNA4/myc-HisCCMV-Profor(777bp)/T7(863bp)BGH(1103bp)/c-mycrev(1024bp)Amp+5079pcDNA4/TOTREfor(908bp)/CMV-Profor(777bp)BGH(1106bp)Amp+5078pcDNA4/V5-HisACMV-Profor(777bp)/T7(863bp)BGH(1117bp)/V5rev(1044bp)Amp+5092pcDNA4/V5-HisBCMV-Profor(777bp)/T7(863bp)BGH(1117bp)/V5rev(1044bp)Amp+5092pcDNA4/V5-HisCCMV-Profor(777bp)/T7(863bp)BGH(1117bp)/V5rev(1044bp)Amp+5092pcDNA5/FRTCMV-Profor(777bp)/T7(863bp)BGH(1039bp)Amp+5069pcDNA6/myc-HisACMV-Profor(777bp)/T7(863bp)BGH(1099bp)/c-mycrev(1020bp)Amp+5126pcDNA6/myc-HisBCMV-Profor(777bp)/T7(863bp)BGH(1099bp)/c-mycrev(1020bp)Amp+5126pcDNA6/myc-HisCCMV-Profor(777bp)/T7(863bp)BGH(1099bp)/c-mycrev(1020bp)Amp+5126pcDNA6/V5-HisACMV-Profor(777bp)/T7(863bp)BGH(1121bp)/V5rev(1048bp)Amp+5148pcDNA6/V5-HisBCMV-Profor(777bp)/T7(863bp)BGH(1121bp)/V5rev(1048bp)Amp+5148pcDNA6/V5-HisCCMV-Profor(777bp)/T7(863bp)BGH(1121b