不同年龄小鼠缺血性心力衰竭后心室重塑程度的比较

1000-4718201306-0988-052012-11-132013-03-11*973No．2010CB535013No．81060025No．20106517110002No．2010YFY25△[e-mail]*沈鑫，马依彤△，杨毅宁，刘芬，于子翔，陈邦党，陈铀，黄莺830054。318C57BL/6J10050240108LVMIⅠ、ⅢMassonCVF。18%vs10%22%vs10%P＜0.05P＜0.05LVMIP＜0.05CVF、ⅠⅠ/ⅢP＜0.05。。Ｒ541．4Adoi10．3969/j．issn．1000-4718．2013．06．005EffectsofdifferentagesonventricularremodelingafterischemicheartfailureinmiceSHENXinMAYi-tongYANGYi-ningLIUFenYUZi-xiangCHENBang-dangCHENYouHUANGYingFirstDepartmentofCoronaryHeartDiseasetheFirstAffiliatedH[e-mail]entricularremodelinginducedbyischemicheartfailureinthemiceatdifferentages．METHODSThree-month-oldyounggroupn=50and18-month-oldoldgroupn=50maleC57BL/6Jmicewereselectedinthestudy．Fortymiceunderwentligationofleftcoronaryarterywithcertaininfarctsizeand10weresham-operatedforcontrol．Echocardiographywasperformedafter8weeksofinfarction．Allmicewerekilledandtheheartswerecollectedforexaminations．Massontrichromestainingwasusedtodetectmyocardialfibrosis．TheexpressionoftypeIandtypeⅢcollagenswasmeasuredbythemethodofimmunohistochemistry．ＲESULTSTheincidencesofcardiacrupture18%vs10%P＜0.05andheartfailure22%vs10%P＜0.05weresignificantlyhigherinagedmicethanthoseinyoungmice．ThedegreesofleftventriculardilationcontractiledysfunctionandheartrateweresignificantlyhigherinagedmicethanthoseinyoungmiceP＜0.05．TheleftventricularmassindexcollagenvolumefractiontheexpressionoftypeIcollagenandratiooftypeⅠ/ⅢcollagensweresignificantlyincreasedinagedmiceascomparedwithyoungmiceP＜0.05．CONCLUSIONAfterheartfailureagedmiceshowabnormalcollagendistributionandsufferfromworsecardiacfunctionsandmoreseriousventricularremodeling．KEYWOＲDSHeartfailureVentricularremodelingAgeCollagenheartfailureHF80%651。·889·ChineseJournalofPathophysiology2013296988-992。。1、C57BL3185010020～30g12h∶12h。MassonⅠ、ⅢAbcam。Hardvard687Hardvard。Lei-ca。22。100mg/kg、20mg/kg、1．2mg/kg。6～040%～45%。63。28。324h、1、2～812～8。48550015MHzheartrateHＲ、leftventricularend-systolicdiameterLVESD、leftventricularend-diastolicdiameterLVEDD、externalleftventriculardiastolicdiameterEXLVDD、thicknessofanteriorwallofsystoleAwsthicknessofposteriorwallofsystolePwsthicknessofanteriorwallofdiastoleAwdthicknessofposteriorwallofdiastolePwdfractionalshort-eningFSFS%=LVEDD－LVESD/LVEDD×100%3。5、、leftventricularmassindexLVMILVMI=/10%、5μm。HE、Massoncollagenvolumefrac-tionCVFCVF=/Ⅰ、Ⅲ5Image-ProPlus6．0Ⅰ/Ⅲ。6±mean±SDSPSS16．02P＜0.05。124h347d53941～827。48%vs26%、18%vs10%22%vs10%P＜0.051。Figure1．Mortalityofdifferentreasonsinthe2groups．*P＜0.05vsyounggroup．12·989·Figure2．Pathologicalresultsoftheheartsinthe2groups×400．228LVEDDLVESDP＜0.05HＲP＜0.05FSP＜0.05HＲFSPwdP＜0.051。18Table1．Echocardiogramresults8weeksafteroperationinthe2groupsMean±SDGroupnHＲmin－1LVEDDmmLVESDmmEXLVDDmmPwsmmPwdmmFS%YoungControl6333±193．45±0．481．54±0．264．77±0．431．40±0．060．73±0．0655．5±3．6HF10394±17*5．00±0．47*4．02±0．33*6．44±0．46*1．49±0．101．11±0．40*19．6±1．5*OldControl6368±403．35±0．281．85±0．224．83±0．351．35±0．150．80±0．1144．8±6．8HF10431±39＊△5．97±0．48＊△5．29±0．46＊△6．96±0．46＊△1．42±0．210．80±0．11△11．6±0．8＊△*P＜0.05vscontrolatthesameage△P＜0.05vsHFinyounggroup．3、P＜0.05LVMIP＜0.05P＜0.05LVMIP＜0.052。CVFP＜0.05Ⅰ、ⅢP＜0.05CVF、ⅠⅠ/Ⅲ·099·P＜0.052ⅢP＞0.052、3。28LVMITable2．OrganweightandLVMI8weeksafteroperationinthe2groupsMean±SD．n=10GroupBWgLVmgＲVmgATmgHeartmgLivermgLVMIYoungControl25．3±1．763．5±3．917．1±2．17．4±1．588．0±6．0981．0±77．72．52±0．23HF26．2±2．2138．5±16．7*40．1±5．9*25．9±5．7*204．5±23．7*892．3±83．0*5．32±0．76*OldControl28．8±1．778．8±9．619．1±2．88．6±1．4107．5±13．11131．9±51．02．75±0．42HF27．8±1．4*166．8±16．2＊△41．0±15．3*22．3±5．6*230．1±25．0＊△954．1±75．9*6．00±0．54＊△LVleftventricleＲVrightventricleATatrium．*P＜0.05vscontrolatthesameage△P＜0.05vsHFinyounggroup．38CVF、ⅠⅢⅠ/ⅢTable3．CVFcollagenⅠcollagenⅢandcollagenⅠ/Ⅲratio8weeksafteroperationinthe2groupsMean±SD．n=5GroupCVF%CollagenⅠ%CollagenⅢ%CollagenⅠ/ⅢYoungControl3．08±0．4610．61±3．009．48±2．571．23±0．60HF23．14±2．49*21．55±2．43*14．82±2．34*1．47±0．16OldControl4．11±0．8711．25±1．8010．61±3．661．22±0．64HF36．89±7．44＊△34．66±3．47＊△16．96±2．07*2．06±0．22＊△*P＜0.05vscontrolatthesameage△P＜0.05vsHFinyounggroup．。4-6。。、、7。、。。extracellularmatrixECM、。Ⅰ、Ⅲ90%ⅠⅢ8-9ECM。2～3410。LVMI。Ⅰ、ⅢⅠ、Ⅰ2ⅢⅠ/Ⅲ11Ⅰ、Ⅲ35dⅠ3dⅢ35dⅢ3d。·199·12ⅢⅠⅠ/Ⅲ。。。1ClinicalQualityImprovementNetworkInvestigators．Mor-talityriskandpaternsofpracticein4606acutecarepa-tientswithcongestiveheartfailuretherelativeimportanceofagesexandmedicaltherapyJ．ArchInternMed1996156151669-1673．2DuXJGaoXMＲamseyD．Surgicalmethodsofindu-cingtransverseaorticstenosisandmyocardialinfarctioninthemouseJ．AsiaPacificHeartJ199873187-192．3GaoXMDartAMDewarEetal．Serialechocardio-graphicassessmentofleftventriculardimensionsandfunc-tionaftermyocardialinfarctioninmiceJ．CardiovascＲes2000452330-338．4vandenBorneSWDiezJBlankesteijnWMetal．Myo-cardialremodelingafterinfarctiontheroleofmyofibro-blastsJ．NatＲevCardiol20107130-37．5CleutjensJPVerluytenMJSmithsJFetal．CollagenremodelingaftermyocardialinfarctionintheratheartJ．AmJPathol19951472325-338．6．－J．200686171155-1157．7SakataYYamamotoKManoTetal．Activ