伯乐-Mini-Opticon荧光定量PCR仪操作说明

MiniOpticon™PCROpticonMonitor™Version3.1CFD3120MiniOpticonMiniOpticon™PCRCFD3120MiniOpticonPTC0148MJMinirSupportsOpticonMonitor™version3.1©2005Bio-RadLaboratoriesChill-out,Chromo4,Hard-ShellMicrosealMiniOpticonMJMinMultiplateOpticonOpticonMonitorAmplifluorIntergenCompanyLUXInvitrogenCorporationExcelMicrosoftWindowsXPScorpionsDXSLtdSYBRMolecularProbes,IncTaqManRocheMolecularSystems,IncVICAppleraCorp.polymerasechainreactionPCRRocheMolecularSystems,IncMiniOpticonAppliedBiosystems.PCRSerialNo__________________PCRNos.4,683,195,4,683,202and4,965,188,PCR10954revA20005~31oC80%31oC100~24050~60400II2FCC1512EMC(CanadianInterference-CausingEquipmentRegulations)FCCFCC15FCC1547CFR§15.103(c)11.1MiniOpticonPCRBio-RadMiniOpticonPCRPCRPeltier480.2mlPCR48PCRPCRLED470~505nm523~543nmSYBRGreenFAM540~700nmCalculatedModeBlockMode31.8×17.8×33cmMJMINI-48PCRMiniOpticonPCR1.2MiniOpticonPCR2345~8MiniOpticonPCR910MiniOpticon1.32MiniOpticon2.1LEDUSBAUSBB2.1MiniOpticon2.2MiniOpticon2.20~99oC30oC±0.2oC90oCNIST±0.4oC90oC102.5oC/48PCR60.2ml8PCR480.2mlPCR100~24050~604002.5250V5×20mm15××32cm×18cm×33cm470~500nm1523~543nm2540~700nm2.3PCR±0.2oC10NIST±0.4oC10NIST±0.4oCNIST35oC99oC1~16oC2.4WindowsXPCPU1.5GHz1024×768512MRAM20GB33.1MiniOpticonPCR3.1.11.MiniOpticonPCR2.USBA3.2MiniOpticonPCRMiniOpticon2-2110V220V3.2.23.2.1MiniOpticonPCR20005~31oC80%31oC±10%IIII31oC3.2.2MiniOpticonPCR100~24050~60±10%90264MiniOpticonPCRIEC60320-1C13150250101203.2.32-12-231oC3.2.3.12-1MiniOpticonPCR10cm3.2.3.2MiniOpticonPCR31oCMiniOpticonPCR3031oC31oC3-13-13.2.4OpticonMonitorTM1.Install2.3.2.5OpticonMonitorTMUSBUSBUSB3.3MiniOpticonPCR2-21OpticonMonitorTMLED3.4/MiniOpticonPCROpenClose3.5948444.1MiniOpticonPCR48470~505nm1523~543nmSYBRGreenIFAM2540~700nmMiniOpticonPCR104.2MiniOpticonPCRPCRSYBRGreenITaqManAmplifluorLUX888-652-92534.2.1SYBRGreenISYBRGreenIDNADNAPCRSYBRGreenI497nm520nmSYBRGreenIPCRDNAPCRPCRSYBRGreenISYBRGreenISYBRGreenI5%DMSOSYBRGreenI888-652-92534.2.2MolecularBeaconFRET15~305~7SYBRGreenISNPDNAPublicHealthResearchInstituteofNewYork,NYMiniOpticonPCR470~505nm523~543nm540~700nmFAM4.2.3TaqManTaqManTaqManDNA5’3’TaqMan3’5’TaqManPCRTaqManDNAMiniOpticonPCRTaqMan4.2.24.2.4ScorpionsProbePCRPCRPCRDNAPCRDNAPCRPCRPCR4.2.24.2.5AmplifluorAmplifluorAmplifluorUniversalDetectionSystemIntergen5’18Z3’PCRAmplifluor4.2.24.2.6LUXLUXLightUponeXtensionFluorogenicPrimerInvitrogenPCRLUX4.2.24.3PCRMiniOpticonPCR480.2mlPCR0.2mlPCRPCR4.5MiniOpticonPCR0.2mlPCR4.4PCRPCRChill-out™4.4.1PCRPCR100oCPCRPCR4.4.2Chill-out™Chill-out™10oC1~315~50µlPCR4.5PCRMiniOpticonPCR888-729-2165888-729-21660.2ml8TBS-0201TLS-0801TLS-0851MultiplateMLP-48014848MLP-240124MultiplateMLL-48014848MLL-4851488TCS-0803PCR5µlChill-outCHO-1411PCR2µl4.6PCRMiniOpticonPCR20~100µlPCR10µlPCR100µl6.4.35OpticonMonitorTM5.1MiniOpticonPCROpticonMonitorTM6PCR783.05.2OpticonMonitorPCR1.PCRMasterFilePCR2.3.CTDNA-dl/dtTm5.3OpticonMonitorTMMiniOpticonPCROpticonMonitor3.2.4OpticonMonitorWindowsStartProgrammeOpticonMonitor3.0MasterMasterFileWindowMaster.mast.platePCR.prot.tadOpticonMonitor5-1OpticonMonitor5.4OpticonMonitorTMOpticonMonitor//MeltCurveMaster5-25-37Master5-35.5OpticonMonitorTMFileExit×5.6OpticonMonitorTMOpticonMonitor.mastMasterPCR.plate.protPCRPCR.tad5.7OpticonMonitorTMHelpAboutOpticonMonitor3.05.8ViewMessageLogViewErroLog66.1MiniOpticonPCRPCR.platePCR.protPCRPCRPCRMasterMasterPCRPCRMasterPCRMaster6.2MasterMasterFileWindowOpticonMonitorMasterMasterMasterMaster6-1MasterMasterPrepareNewRunMasterPCRMasterMasterReuseMasterFile6.2.1MasterMasterMasterFilePanelMasterQuickLoadMenu6.2.4MasterInstrumentPanel6.2.3PlateSetupPanelQuickLoadMenu6.2.4MasterPCR6.3.15PCRProtocolSetupPanelPCRQuickLoadMenu6.2.4PCRPCR6.4.86.2.2Master6.2.2.1Master6-2ManageUsersWindowAdd6.2.2.2Master6.2.2.3Remove6.2.3OpticonPCRScanForInstrumentReinitializeInstrumentSetDefaultInstrumentMiniOpticonPCRMiniOpticonPCR6.2.4QuickLoadMenuMasterPCRPlate6-3DataFiles6.36.3.1.plate1OpticonMonitor2346.3.2MasterNewEdit6-46.3.3PlateSetupWindow486.3.66.3.7PlatePlateTypeDyesPanelContentsPanelDisplayPanelWellsSetsWellsSets6.3.4OpticonMonitorFAMSYBRGreenIHEXTETVICPlateTypeDyesPanelContentsPanelDisplayPanelDeselectAllSelectinUseAFAMVICBSYBRGreenIHEX6.3.4.1470~500nm106.3.56-466-4A4~C6912CtrlCtrlCtrlCtrl6.3.6EmptyBlankStandardSamplePassiveRefrencePlate6.3.8AB1.2.6.3.53.EBStSaPRPlate6.3.86.3.76-5DefaultModeFixedWellSectionsFixedMode6.3.7.16-5AMiniOpticonPCR6.3.7.2FixedMode6-51~5FAMVIC6SYBRGreenIFixedWellSectionsMiniOpticonPCR6-5B6.3.7.3DeselectAll6-5B66.3.8PlatePassiveRefrencePRE6.3.9DescriptionCtrl+CCtrl+V6.3.10Wells6-4ShowEmptiesWellDyeContentDescription6.3.11WellGroupPCRManageWellGroupManager6-6WellGroupManagerNewNameApplyAddNewEditApplyDeleteOK×OK6-7WellGroupPlate6.3.12OpticonMonitor88Sets6-8NewNewUpdateUpdateNewSetNameNewSet2ReplicateTypeCalibratorCTCT8ControlCTNameUpdateNameRemove6.3.13PCRSpecifyQuantStandardsWellDyeAmount6-9AmountExcel10001E41e+004UnitsngpgmolesmoleculesgegenomeequivalentscopiesOKCancelManagerAddManagerRemove6.3.14MicrosoftExcelExcelExcelOpticonMonitorExcelCtrl+COp