CCK-8说明书-2019.02.22

5.-----6.-®-®-臜--7.-8.9.--10.1.-2.3.-5------4.-12345678910CellCountingKit-8(CCK-8)--产品名称CytotoxicityLDHAssayKit-WST®Viability/CytotoxicityMultiplexAssayKitAnnexinV,FITC/PIApoptosisDetectionKitAnnexinV,633ApoptosisDetectionKitCellCycleAssayKit-PI/RNaseStainingDALGreen(自噬溶酶体)DAPGreen(自噬体及自噬溶酶体)MitophagyDetectionKitCellularSenescenceDetectionKit-SPiDER-βGalCalcein-AM/PIDoubleStainingKitJC-1MitoMPDetectionKitLactateAssayKit-WST®LiperfluoMitoPeDPPMito-FerroGreenGSSG/GSHQuantificationKitII应用细胞毒性检测活细胞/死细胞检测细胞凋亡检测细胞周期检测细胞自噬检测线粒体自噬检测细胞衰老检测活死细胞双染线粒体膜电位检测乳酸检测脂质过氧化物检测铁死亡检测谷胱甘肽检测货号CK12CK17AD10AD11C543D675D676MD01SG03C542MT09L256L248M466M489G263规格500T500T50T50T50T20nmol5nmol1set5assays500T1set50tests50g×55g×350g×2200TMedium:HeLa.......MEM,10%FBSHL60.......RPMI1640,10%FBS[3H]-Thymidine........37KBq/wellReagent:CCK-8.....................10l/well[3H]-Thymidineassay......4hoursIncubation:CCK-8..........................3hoursr=0.99600.10.20.30123AbsorbanceHL60Cellr=0.99900.511.500.511.52[3H]-Thymidinex10-5dpm[3H]-Thymidinex10-5dpmAbsorbanceHeLaCellNumberofCells(x103cells/well)NumberofCells(x103cells/well)01230510152025AbsorbanceHeLaCell00.10.20.30510152025AbsorbanceHL60Cell®®臜NNNNNNNHN-O3S-O3SNO2NO2NO2NO2SO3-SO3-H3COH3CONa++formazanWST®-8WST®-8formazansubstratesdehydrogenasescellNADNADHelectronmediatorreducedformelectronmediatorcolorlessWST®-8orangecolor---®臜®臜臜Medium:HeLa.......MEM,10%FBSHL60........RPMI1640,10%FBSIncubation:HeLa........37,5%CO2,2hoursHL60........37,5%CO2,3hoursDetection:CCK-8()....450nm,XTT()....450nmMTS().......490nm,MTT()...570nm1.KieKyonHuang,etal.,GenomicandEpigenomicProfilingofHigh-RiskIntestinalMetaplasiaRevealsMolecularDeterminantsofProgressiontoGastricCancer,CancerCell,2018,33,1-142.TaChungYu,etal.,FusobacteriumnucleatumPromotesChemoresistancetoColorectalCancerbyModulatingAutophagy,Cell,2017,170(3),548-5633.KunChen,etal.,MethyltransferaseSETD2-MediatedMethylationofSTAT1IsCriticalforInterferonAntiviralActivity,Cell,2017,170(3),492-5064.Mitochondrialunfoldedproteinresponsecontrolsmatrixpre-RNAprocessingandtranslation,Nature,2016,534,710–7135.Transferofmitochondriafromastrocytestoneuronsafterstroke,Nature,2016,535,551–5556.Whole-genomemutationallandscapeandcharacterizationofnoncodingandstructuralmutationsinlivercancer,NatureGenetics,2016,48,500–5092009,33(4),400-4012008,18(3),313-3052007,16(5),559-5622006,46(6),20-21-2.521.510.500.010.11ConcentrationActinomycinD(g/ml)Absorbanceat450nm1235674Cellline:HeLaCulturemedium:DMEM,10%FBSDrug:ActinomycinDExposure:24hoursIncubation:37,5%CO24hoursDetection:450nm/------------------®--®-®-臜---®