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1,1,2,13(1.,610039;2.,610031),,;;Q786A1005-7021(2005)04-0092-03ApplicationofGeneChipTechnologytoDetectPathogensinFoodHEYang1,ZHOULi2li1,LIUHong2lu2,LIYu2feng1(1.SchoolofBioengin.XihuaUniv.Chengdu610039;2.SichuanProv.Cent.ofDiseasesPreventionandControl.Chengdu610031)AbstractGenechiptechnologyasanewtechnologyplatformhasbeenwidelyusedinfoodstudy.Withthecontinu2ousperfectionofthistechnology,itwillplaymoreandmoreimportantroleinfoodstudyandfoodsafety.Basicprin2ciple,detectionprocess,unsolvedproblems,researchprogressofitcommoninfooddetectionwereintroduced.Keywordsgenechip,pathogen,detection(genechip)2090,,,,DNA,,,PCRDNADNADNA,3,DNA3:DNADNADNA,,DNA1,DNAPCR,,[1]22.1,:2005-04-01:,:(04JY029205321)32920057254JOURNALOFMICROBIOLOGYJuly2005Vol.25No.42.2,,,PCR,,(12),PCR(PCR24h,),,2PCR;,,,,,2.31996,DNA,[2],[3]:,;,;,,,,,2DNA[4];[5]:,;[6]3h;[7],,126dBorucki[8];Volokhov[9]6;Call[10]E.coliO157:H7Shiga,ShigaA,E.coliO157:H7Jack[11]16SrRNA,,Wilson[12]20,,182.42.4.1PCR16SrRNA,,16SrRNA,16SrRNA,,16SrRNAPCR,,2.4.2[13],DNA,DNA:,[14];,[15][16],394:,,2.4.3,DNA,(PCR),2.0%,2.4.4DNA,DNA,DNADNA,,2.4.5,,3,,,:,,,;,,;,,,,;,,21,,,,,:[1]StearsRL,etal.Trendsinmicroarrayanalysis[J].NatMed,2003,9(1):140-145.[2]GallM,LabadieJ,BrockP,etal.Light2directedsynthesisofhighdensityoligonucleotidearraysusingsemiconductphotore2sis[J].ProNatlAcadSciUSA,1996,20:13555-13560.[3]JordanB.Large2scaleexpressionmeasurementbyhybridizationmethod:fromhigh2densitymembranestoDNAchips[J].JapBiochemSoc,1998,24:215-218.[4],,,.[J].,2002,12(2):254-255.[5],,,.[J].,2002,36(4):238.[6],,,.[J].,2004,22(1):24-26.[7].12[J].,2003,24(6):288.[8]BoruckiMk,etal.DiscriminationamongListeriamonocyto2genesisolatesusingamixedgenomeDNAmicroarray[J].VetMicrobiol,2003,92:351-342.[9]VolokhovD,etal.IdentificationofListeriaspeciesbymicroar2ray2basedassay[J].JClinMicrobiol,2002,40:4720-4728.[10]CallDR,etal.DetectingandgenotypingEscherichiacoliO157:H7usingmultiplexedPCRandnucleicacidmicroarray[J].IntJFoodMicrobiol,2001,67:71-78.[11]JackS,DouglesRC,FredJB,etal.Directdetectionof16srRNAinsoilextractsbyusingoligonucleotidemicroarrays[J].App.Env.microbiol,2001,67:4708-4716.[12]WilsonWJ,etal.Sequence2specificidentificationof18patho2genicmicroorganismsusingmicroarraytechnology[J].MolCellProbes,2002,16:119-127.[13],,,.[J].,2000,30(5):1-6.[14]SchenaM,ShalonD,HellerR,etal.Parallelhumangenomeanalysis:microarraybasedexpressionmonitoringof1000genes[J].Proc.Natl.Acad.Sci.USA,1996,93:10614-10619.[15]BlanchardAP,KaiserRJ,HoodLE.Highdensityoligonu2cleotidearrays[J].Biosensors&Bioelectronics,1996,11:687-690.[16]McGallG,LabadieJ,BrockP,etal.Lightdirectedsynthesisofhighdensityoligonucleotidearraysusingsemiconductorpho2toresists[J].Proc.Natl.Acad.Sci.USA,1996,93:13555-13560.4925