低聚木糖的制备及其对益生菌体外增殖的作用

浙江大学学报()37(3):245~251,2011JournalofZhejiangUniversity(Agric&LifeSci):10089209(2011)03024507DOI:10.3785/j.issn.10089209.2011.03.002:20100713:(2008C12052).:(1980),,,,.Tel:[phone];Email:[e-mail].:,,,.Tel:[phone];Email:[e-mail].李艳丽1,许少春1,柳永1,杨会玲2,许尧兴1(1.,310021;2.,712100):针对玉米芯微波消解内切木聚糖酶水解制备低聚木糖的工艺,以低聚木糖的得率为主体评价指标,通过单因素实验对影响低聚木糖得率的微波消解过程和内切木聚糖酶水解过程的因素与水平进行研究,并考察获得的低聚木糖对益生菌的体外增殖作用.结果表明:玉米芯酶法制备低聚木糖的最佳工艺条件为微波处理压力1.6MPa,微波处理时间5min,内切木聚糖酶用量140Ug-1,酶解时间6h;在最适条件下,玉米芯酶解液中低聚木糖的得率为82.5%,质量浓度为11.02gL-1;低聚木糖对益生菌的体外增殖实验表明,低聚木糖添加量在02%和04%时,分别可以对乳酸杆菌和枯草芽孢杆菌起到最好的增殖作用,分别达到311%和183%,添加量进一步提高反而会抑制这2种菌的生长.:低聚木糖;微波消解;内切木聚糖酶;枯草芽孢杆菌;乳酸杆菌:Q814:ALIYanli1,XUShaochun1,LIUYong1,YANGHuiling2,XUYaoxing1(1.InstituteofPlantProtectionandMicrobiology,ZhejiangAcademyofAgriculturalSciences,Hangzhou310021,China;2.CollegeofAnimalScienceandTechnology,NorthwestA&FUniversity,Yangling,Shaanxi712100,China)Preparationofxylooligosaccharidesanditseffectoninvitroproliferationofprobiotics.JournalofZhejiangUniversity(Agric&LifeSci),2011,37(3):245251Abstract:Xylooligosaccharides(XOS)fromcorncobwerepreparedbymicrowavedigestionandacommercialendoxylanasehydrolysis.OnefactoratatimemethodwasappliedtooptimizethereactionparametersforimprovingXOSconcentration,andtheeffectofobtainedXOSoninvitroproliferationofprobiotics(BacillussubtilisandLactobacillus)wasstudied.Theresultsshowedthattheoptimalconditionswereasfollows:microwavedigestionpressureof16Mpaandmicrowavedigestiontimeof5min,endoxylanaseconcentrationof140Ug-1andenzymaticreactiontimefor6h.Underoptimalconditions,hydrolysisofalkalipretreatedcorncobpowderusingmicrowavedigestionandendoxylanaseproduced825%ofXOSinthehydrolyzate,equivalentto1102gL-1XOS.InvitroproliferationsofLactobacillusandB.subtiliswereactivatedby311%and183%withtheadditionof02%and04%XOS,respectively,howevertheirgrowthswereinhibitedwithincreasingtheamountofXOSaddition.Keywords:xylooligosaccharide;microwavedigestion;endoxylanase;Bacillussubtilis;Lactobacillus浙江大学学报()2~71,4,,,40%.pH,(pH=25).,,,,[1]..6[26]:,,;;,;,,;,;,.,.35%,.,,,.,[79].,.,,,,.1材料与方法11:60F254();:;D,,:WAKO;:Sigma;:,;:MDS2003F,;:,2mm,w()=759%,w()=237%.12DNS[10].:pH5050,1min1mol,U.13.:,1010%NaOH,60,pH,60.:05g,10mL,,.:10mL,,16h;.14:,,V()V()V()=2113~4h,602~3h,,8510min.[11]::Waters515;:Waters2410;:RT30078PolyspherCHPB(78300mm,);:;:04mLmin-1;:90;:50L.15[1213].LuriaBertani(LB),deManRogosa24637,:Sharpe(MRS).:LB01%~05%,1%,30200rmin-120h,,.:3748h,.2结果与分析21,,,,,.,,,;,,100Ug-1,16h.1:08MPa15~45min,,,,;20MPa3min,,,,,0675gL-1,.,.1Table1Effectofmicrowavedigestionpressureandtimeonxylooligosaccharidesproduction/MPa/minw()/(gL-1)w()/(gL-1)w()/(gL-1)12345678910111213141508080812121216161616161620202015304515304558121530453580000051200011374005071700216500081793006241401257880215820018211001128200113352014437800947090094983010578000863090117193007724101384110158766021878101894910239588019884101494380161067021003500010037000200390001003800010159000701840006016700080231000603520003072100111031001216110013067500070862000509260013,,5713,(2),7,(6641013)gL-1,8143%,5,8231%,(6309011)gL-1,13,57,(3609007)gL-1,,(8841014)gL-1,(5441014)gL-1.,20MPa(3min),,2473浙江大学学报(),,,16MPa,5min,(0512gL-1),6309gL-1,(0159gL-1).2Table2Effectofmicrowavedigestionconditiononxylooligosaccharidescontentsw()/(gL-1)w()/(gL-1)w()/(gL-1)w()/%w()/%5713630901172410138841014135600615140023609007630901166410135441014176918573988823181436012,,(1).1:;2:16MPa,5min,;3:16MPa,5min,;4:20MPa,3min,;5:20MPa,3min,.1TLCFig.1TLCchromatographyofcorncobproductionobtainedbymicrowavedigestionandendoxylanasehydrolysis22,D1,4D1,4[14],D1,4pH40~50,pH30~40,pH40,pH70,pH50,,pH50~60.Jeong[15],,60,;Yang[16]50.pH40~7040~60.3.4,pH,C3A3B4C7B3,C3B1B7B2B3A3,,3pH50pH,,C3974gL-1,880%,pHpH5060.60,pH5065,4D3.D3C3,C3,pH5060.3pHTable3TableheaderdesignoftemperatureandpHforxylandigestion/pH4045505560657040A1A2A3A4A5A6A750B1B2B3B4B5B6B760C1C2C3C4C5C6C724837,:4pHTable4EffectofpHandtemperatureonxylooligosaccharideproductionw()/(gL-1)w()/(gL-1)w()/%w()/%A1A2A3A4A5A6A7B1B2B3B4B5B6B7C1C2C3C4C5C6C7D31610011500021810022230012590052280041740021210011280011720051930032060051650031240011430021980051330043270023110041910062130011990066340096160118700098108?01167160?01067158?01127138?01077148?01116180?01138148?01158166?01148140?01087152?01047153?01087106?01056130?01119174?01137128?01107170?01087154?01098152?01117193?011020131914171221162514231119111319151816191812191918101411161823191210311028182012201020117917791682187814741676198019861184128311811881118210851983127611881069107112791880107919213116MPa5min(g),60e8h.(5),80U!g-1,(5121?0107)g!L-1,140U!g-1,(10153?0114)g!L-1,,,140U!g-1,8113%.5Table5Effectofenzymeconcentrationsonxylo2oligosaccharideproduction/(U!g-1)w()/(g!L-1)w()/(g!L-1)w()/%w()/%801001201401601802001145?01021152?01062158?01032142?01013140?01053121?01042153?01055121?01076173?01048102?011010153?011410176?010910190?011111103?0113211818142413181224102217181778128116751781137610771381132493浙江大学学报()6.6h,11102g!L-1.2h,83.0%,7121g!L-1,,,4h,.,,,,,.6Table6Effecto