常见酶切位点及其保护碱基

andyyuyuhe1982
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2020-09-25

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资源描述

ChainLength2hr20hrGGTCGACC800CGGTCGACCG1000CCGGTCGACCGG1200CACATGTG800CCACATGTGG109090CCCACATGTGGG129090GGCGCGCC89090AGGCGCGCCT109090TTGGCGCGCCAA129090CCCCGGGG85090CCCCCGGGGG109090TCCCCCGGGGGA129090CGGATCCG81025CGGGATCCCG109090CGCGGATCCGCG129090CAGATCTG800GAAGATCTTC107590GGAAGATCTTCC122590GGCGCGCC800AGGCGCGCCT1000TTGGCGCGCCAA125090BstEIIGGGT(A/T)ACCC9010AACTGCAGAACCAATGCATTGG2200AAAACTGCAGCCAATGCATTGGAA242550CTGCAGAACCAATGCATTGGATGCAT272590CATCGATG800GATCGATC800CCATCGATGG109090CCCATCGATGGG125050GGAATTCC89090CGGAATTCCG109090CCGGAATTCCGG129090GGGGCCCC89090AGCGGCCGCT109090TTGCGGCCGCAA129090CAAGCTTG800CCAAGCTTGG1000CCCAAGCTTGGG121075GGGTACCC800GGGGTACCCC109090CGGGGTACCCCG129090GACGCGTC800CGACGCGTCG102550CCCATGGG800CATGCCATGGCATG145075CCATATGG800CCCATATGGG1000CGCCATATGGCG1200GGGTTTCATATGAAACCC1800GGAATTCCATATGGAATTCC207590GGGAATTCCATATGGAATTCCC227590GGCTAGCC800CGGCTAGCCG101025CTAGCTAGCTAG121050TTGCGGCCGCAA1200AscIEnzymeOligoSequence%CleavageAccIAflIIINcoIAvaIBamHIBglIIBssHIIBstXIClaI常用酶切位点的保护性碱基NdeINheINotIEcoRIHaeIIIHindIIIKpnIMluIATTTGCGGCCGCTTTA161010AAATATGCGGCCGCTATAAA201010ATAAGAATGCGGCCGCTAAACTAT242590AAGGAAAAAAGCGGCCGCAAAAGGAAAA282590TGCATGCATGCA121090CCAATGCATTGGTTCTGCAGTT229090TTAATTAA800GTTAATTAAC10025CCTTAATTAAGG12090GTTTAAAC800GGTTTAAACC10025GGGTTTAAACCC12050AGCTTTGTTTAAACGGCGCGCCGG247590GCTGCAGC800TGCACTGCAGTGCA141010AACTGCAGAACCAATGCATTGG229090AAAACTGCAGCCAATGCATTGGAA249090CTGCAGAACCAATGCATTGGATGCAT2600CCGATCGG800ATCGATCGAT101025TCGCGATCGCGA12010SacICGAGCTCG81010GCCGCGGC800TCCCCGCGGGGA125090GTCGACGTCAAAAGGCCATAGCGGCCGC2800GCGTCGACGTCTTGGCCATAGCGGCCGCGG301050ACGCGTCGACGTCGGCCATAGCGGCCGCGGAA321075GAGTACTC81025AAAAGTACTTTT127575CCCGGG6010CCCCGGGG8010CCCCCGGGGG101050TCCCCCGGGGGA129090GACTAGTC81090GGACTAGTCC101090CGGACTAGTCCG12050CTAGACTAGTCTAG14050GGCATGCC800CATGCATGCATG12025ACATGCATGCATGT141050AAGGCCTT89090GAAGGCCTTC109090AAAAGGCCTTTT129090CTCTAGAG800GCTCTAGAGC109090TGCTCTAGAGCA127590CTAGTCTAGACTAG147590CCTCGAGG800CCCTCGAGGG101025CCGCTCGAGCGG121075CCCCGGGG800CCCCCGGGGG102575CCCCCCGGGGGG125090TCCCCCCGGGGGGA149090XhoIXmaIPstIPvuISacIISalIScaISmaISpeISphIStuIXbaINotINsiIPacIPmeI