沙门氏菌的血清学鉴定

北京陆桥技术有限责任公司IdentificationIdentificationSerotypingSerotypingofSalmonellaofSalmonella沙门氏菌的血清学鉴定沙门氏菌的血清学鉴定北京陆桥技术有限责任公司技术服务电话：[phone]技术部邮箱：[e-mail]销售服务电话：[phone]北京陆桥技术有限责任公司主要内容1沙门氏菌属的分类及其抗原简介2泰国S&A沙门氏菌抗血清产品介绍3沙门氏菌的血清学分型方法北京陆桥技术有限责任公司沙门氏菌种及亚种中的血清型数量YEAR199820012007speciesI.Salmonellaenterica种I.肠道沙门氏菌2,4432,4802,557subspeciesenterica肠道亚种1,4541,4781,531subspeciessalamae萨拉姆亚种489498505subspeciesarizonae亚利桑那亚种949499subspeciesdiarizonae双亚利桑那亚种324327336subspecieshoutenae豪顿亚种707173subspeciesindica印度亚种121213speciesII.Salmonellabongori种II.邦戈尔沙门氏菌202122Total(GenusSalmonella)2,4632,5012,579北京陆桥技术有限责任公司ConsistsofTwospecies:1.S.enterica2.S.bongori(V)1.S.entericawhichisdividedintoSixsubspeciesS.entericasubsp.enterica(I)S.entericasubsp.salamae(II)S.entericasubsp.arizonae(IIIa)S.entericasubsp.diarizonae(IIIb)S.entericasubsp.houtenae(IV)S.entericasubsp.Indica(VI)沙门氏菌属的分类北京陆桥技术有限责任公司利用生化特性区别沙门氏菌属的种及亚种*北京陆桥技术有限责任公司SalmonellaSalmonellaspp.spp.O-antigensVi-antigensO-antigenorSomaticantigenistheoneofcellwallcomponent.O抗原也称菌体抗原，是细胞壁的组成成分。ThereactionofO-antigenwithantiserawillbethegranularform.O抗原与抗血清的反应呈颗粒状。Vi-antigenisaspecialsomaticantigenandbelongstothegroupofKantigens.Vi抗原是一种特殊的菌体抗原，属于K抗原群SuchasSalmonellaTyphi,ParatyphiCandDublin.如伤寒沙门氏菌、丙型副伤寒沙门氏菌和都柏林沙门氏菌都含有Vi抗原H-antigenofFlagellaantigenThereactionofH-antigenwithantiserawillbethefloccularform.H抗原与抗血清的反应呈絮状或绒状。AlmostSalmonellahas2phaseofH-antigenthefirstphasecalledspecificphaseandanotherphasecallnon-specificphase.大部分沙门氏菌H抗原都有两相，第一相被称为特异性相，第二相为非特异性相北京陆桥技术有限责任公司SalmonellaSalmonellaspp.spp.————OO抗原抗原„O抗原比较耐热，100℃1h不被破坏，也不易被酒精破坏；„沙门氏菌属抗原表中，O抗原以阿拉伯数字表示，从1排到67，但删去了9个抗原，现在有58个抗原；„凡含有群特异性共同抗原的菌型归类为一个O群，以主要抗原来表示，某些次要抗原可出现在多个群中。北京陆桥技术有限责任公司OldnewOldnewOldnew其他A2G1-G213Q39R40S41T42U43V44W45X47Y48Z5051-67B4H6,14C1-C46,7I16C2-C38J17D19K18D29,46L21D39,46,27M28E1-E2-E33,10N30E41,3,19O35F11P38沙门氏菌O群的命名北京陆桥技术有限责任公司SalmonellaSalmonellaspp.spp.————HH抗原抗原„鞭毛抗原主要成分是蛋白质，不耐热，易被酒精破坏；„沙门氏菌属中有单相菌，但大多数是双相菌，有的第二相抗原中也含有第一相中的e,n,x,z,l,w等抗原；„第一相（特异相）：用小写英文字母a-z表示，再以z1-z83补充；„第二相（非特异相）：用阿拉伯数字表示，仅列出H2、H5、H6、H7抗原，且以H1、2；H1、5；H1、6；H1、7形式出现。北京陆桥技术有限责任公司GroupSerovarO-antigenH–antigenphase1phase2O:2(A)S.ParatyphiAS.Kiel1,2,12a(1,5)O:4(B)S.ParatyphiBS.SchwazengrundS.Typhimuriu1,4,(5)121,4,12,271,4,(5)12bdi-1,71,2O:7(C1)S.CholeraesuisS.Virchow6,76,7cr1,51,2O:8(C2-C3)S.LamphunS.TananariveS.Brunei6,86,88,20yyy1,21,51,5O:9(D1)S.TyphiS.EnteritidisS.Gallinarum9,12(Vi)1,9,121,9,12dg,m----O:3,10(E1)S.WeltevredenS.Ratchaburi3,10,(15)3,10,(15)rz35z61,6O:38(P)S.Bangkok38z4z24-AntigenicFormulaeoftheSalmonellaSerovars,2007北京陆桥技术有限责任公司SalmonellapolyvalentA-Iantisera=Oagglutininsofgr.A+B+C+D+E+F+G+H+IO-PolyvalentantiseraO-groupantiseraO-GroupsPoly.A,gr.B,gr.C,gr.Dgr.E,gr.Fgr.G,gr.H,gr.IAntisera=Sal.poly.A-Iantisera1.沙门氏菌S&A抗血清产品北京陆桥技术有限责任公司Salmonellapoly.OMAantisera=Oagg.ofgr.A+B+D+E+LSalmonellapoly.OMBantisera=Oagg.ofgr.C1+C2+C3+F+G+HO-GroupsPoly.C,gr.Fgr.G,gr.HAntiseraO-GroupsPoly.A,gr.B,gr.Dgr.E,gr.LAntiseraSalmonellapoly.OMAantisera=2.Salmonellapoly.OMBantisera3.=O-PolyvalentantiseraO-groupantisera北京陆桥技术有限责任公司Salm.poly.OMCanti.=Oagg.ofgr.I+J+K+M+N+O+PO-GroupsPoly.I,gr.J,gr.Kgr.M,gr.N.gr.O,gr.PAntiseraSalm.poly.OMDanti.=Oagg.ofgr.Q+R+S+T+U+V+WO-GroupsPoly.Q,gr.R,gr.Sgr.T,gr.U,gr.V,gr.WAntiseraSalm.poly.OMCanti.==Salm.poly.OMDanti.4.5.O-PolyvalentantiseraO-groupantisera北京陆桥技术有限责任公司Salm.poly.OMEanti.=Oagg.ofgr.X+Y+Z+O:51+O:52+O:53+O:61O-PolyvalentantiseraO-groupantiseraSalm.poly.OMEanti.Ogrouppoly.gr.X,gr.Y,gr.Z,gr.O:51,gr.O:52,gr.O:53,gr.O:617.6.Sal.poly.OMFanti.=Oagg.ofgr.O:54+O:55+O:56+O:57+O:58+O:59O-GroupsPoly.gr.O:54,gr.O:55,gr.O:56,gr.O:57,gr.O:58,gr.O:59AntiseraSal.poly.OMFanti.==北京陆桥技术有限责任公司O-PolyvalentantiseraO-groupantiseraSalm.poly.OMGantiSalm.poly.OMGanti.=Oagg.ofgr.O:60+O:62+O:63+O:65+O:66+O:67O-GroupsPoly.gr.O:60,gr.O:62,grO:63,gr.O:65,gr.O:66,O:67Antisera8.=北京陆桥技术有限责任公司Ogrouppoly.antiseraOfactorantiseraSal.gr.AO:1,O:2,O:12Sal.gr.BO:1,O:4,O:5O:12,O:27Sal.gr.CO:61,O:62,O:7O:8,O:14,O:20Sal.gr.DVi,O:1,O:9O:12,O:27,O:46北京陆桥技术有限责任公司Ogrouppoly.antiseraOfactorantiseraSal.gr.EO:1,O:10,O:3O:15,O:19,O:34Sal.gr.FO:11Sal.gr.GO:1,O:13,O:22O:23Sal.gr.HO:1,O:6O:14,O:25Sal.gr.IO:16北京陆桥技术有限责任公司1.H-PolyvalentAntisera2.H–Phase&FactorAntisera3.H–PhaseinversionAntisera沙门氏菌S&A抗血清产品北京陆桥技术有限责任公司HMApoly.1.H-PolyvalentAntiseraphase1&2==H:H(Polyvalentphase1&2)=Hagg.of:-HMA+HMB+HMC+HMD+HMF(ExceptHME)H:HPoly.H-factorHMA,HMB,HMC,HMD,HMFantiseraH-factorof:-H:a,H:b,H:c,H:d,H:i,H:z10,,H:z29HMApoly.=Hagg.of:-a+b+c+d+i+z10+z292.HMAPolyvalentAntisera北京陆桥技术有限责任公司HMB=Hagglutininsof:-Ecomplex+Gcomplex3.HMB-PolyvalentAntiseraH-factorof:-H:f,H:g,H:s,H:t,H:m,H:p,H:q,H:uH-factorof:-H:h,H:n,x,H:x,H:z15==GcomplexHMBpolyEcomplexEcomplexGcomplex=北京陆桥技术有限责任公司HMC=Hagg.of:-k+r+y+z+Lcomplex+Z4complex4.HMC-PolyvalentAntiseraLcomplex=Hagg.of:-l+v+w+z13+z28+z40Z4complex=Hagg.of:-z4+z23+z32===HMCpoly.H-factorof:-H:k,H:r,H:y,H:z,H:Lcomplex,H:Z4complex北京陆桥技术有限责任公司HMD=Hagg.of:-z35+z36+z38+z39+z41+z42+z44+z605.HMDPolyvalentAntisera=H-factorof:-H:z52,H: