Ni柱亲和层析

59409100036TEL[phone]FAX[phone]FF6%50mol/ml45mg/ml45-165m300cm/hpH3-10pH2-11+4~820%HisFFHis1FF1.6×20m10ml212-52ml/min320ml(50mMPBSpH7.40.5MNaCl)0.45m1ml/min412-52ml/min59409100036TEL[phone]FAX681617815102050100200300400mMmM32ml/minSDS-PAGE6520%32ml/min+4~871SDS-PAGE2150mMpH7.4PBS0.5MNaH2PO419ml0.5MNa2HPO481mlNaCl29.3g,1000ml250mMpH7.4pH7.4PBS0.5MNaH2PO419ml0.5MNa2HPO481mlNaCl29.3g34g,1000ml3B1(ml)2(ml)10mM98220mM96450mM9010100mM8020200mM6040300mM4060400mM2080SDS-PAGE1BCA25-10µg/31.5mlEP5-10µg10µl20mMPBSpH7.410µl10µl1ml201h410000rpm15min3710min520mMPBSpH7.42loadingbuffer10µl10010min30s4000rpm1s6HisFFHis20ml102050100200300400mMB3SDS-PAGE41653241.FFHis1234567892.SDS-PAGE123420mM550mM6100mM7200mM8300mM9400mMHis8M346M8M150mMpH7.4PBS0.5MNaH2PO419ml0.5MNa2HPO481mlNaCl29.3g480g1000ml59409100036TEL[phone]FAX[phone]mMpH7.4pH7.4PBS0.5MNaH2PO419ml0.5MNa2HPO481mlNaCl29.3g34g480g1000ml3B1(ml)2(ml)50mM9010400mM20803FF12344SDS-PAGE12350mM4400mMFFHis10ml2050100200500mM3121%8090%80%59409100036TEL[phone]FAX6816178159409100036TEL[phone]FAX[phone]FFIDANi-IDANi-NTANi-IDANi-NTANi-IDANi-NTANTAIDANTANTA50-100mM30-40mM500mMNTANTA1)12340.3MPa300cm/h75%2122-5342-5552-5659409100036TEL[phone]FAX[phone]M0-50mM0-2MNH4ClpH3EDTAEGTA40.15-0.5MNaCl5Cu2+pH50mMNa2HPO40.5MNaClpH7.450mMNa2HPO40.5MNaClpH3.550mMNa2HPO41MNaClpH7.450mMNa2HPO41MNH4ClpH7.450mMNa2HPO40.5MNaClpH7.450mMNa2HPO40.5MNaCl50mMEDTApH7.4pHFF1234FF45FF45FF45FF45FF45CS-A01b-00NTAFF12CS-A01b-01NTAFF12CS-A01b-02NTAFF12CS-A01b-03NTAFF12CS-A01b-04NTAFF12010-8963125686329655010-6780454813911415318E-mail:[e-mail]010-68161781