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,2008,29(8):60262ProgressinVeterinaryMedicine31,2,23,2,2,2,2(1.,150030;2.,150001):,,,,RT2PCRRT2PCR(NASBA),:;2;;:S852.659.5:A:100725038(2008)0820060203(Avianinfluenzavirus)A,A,,,[1]A,;,,,1RT2PCRRT2PCR,[2]RT2PCR,RT2PCR,RT2PCRRT2PCR(nestedRT2PCR)PCR(multiplexPCR)RT2PCR,,,StarickE[3],RT2PCRAIV,,PCRRT2PCR,,,,StarickE[3],H7RT2PCR,,,,RT2PCR22,[4]H5H7AIVRT2PCR,,2RT2PCRRT2PCRPCR,,PCREB,[527],TaqManRT2PCR[829],TaqMan53:2008204223:(2007DFR30360):(1980-),,,,3©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(FRET)PCR,Taq53,,FRET,[8]TaqManRT2PCRA,,[9]H5RT2PCR,;,OIE,21d4h,,TaqMan,SYBR2GreenDNA,,,,,[10]SYBR2GreenIRT2PCR,PCR1.5h,RNA4h,3(NASBA)RNA[11],RNA,,,PCR(AMVRNAHT7RNA)2NASBA,5T7RNA,5(ECLprobe)[12],RNART2PCR,NASBA,,,,,(H12H15)(NASBA2AIV)H5(NASBA2H5)H7(NASBA2H7)NASBA[13214]4,,,,,,cDNADNALiJ[15217],26AHA(H1,H2,H3)NA(N1,N2)NP,BHA(H1,H2,H3)NA(N1,N2)NP,;[18219]RT2PCR500bpAIVcDNA,,DNA,,RNA,cy5cDNA,,,H5H7H9AIV[20221]cDNADNA,51878,100,,,,,,,,,,,,,16:©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.:[1].[M].2.:,2002.[2],,.RT2PCR[J].,2004,25(4):53255.[3]StarickE,WernerO.DetectionofH7avianinfluenzavirusdi2rectlyfrompoultryspecimens[J].AvianDis,2003,47(3):118721189.[4],,,.H5H7[J].,2005,35(6):4372440.[5]SmithAB,MockV,MelearR,etal.Rapiddetectionofinflu2enzaAandBvirusesinclinicalspecimensbyLightCyclerreal2timeRT2PCR[J].JClinMicrobiol,2003,28:51258.[6]ChenW,HeB,LiC,etal.Real2timeRT2PCRforH5N1avianinfluenzaAvirusdetection[J].JMedMicrobiol,2007,56:6032607.[7]TraniL,BediniB,DonatelliI,etal.Asensitiveone2stepreal2timePCRfordetectionofavianinfluenzausingaMGBprobeandaninternalpositivecontrol[J].BMCInfectiousDisease,2006,6:87.[8],,,.RT2PCRA[J].,2006,6(1):33235.[9].RT2PCR(HPAIV)H5[D].:,2004:7211.[10],,,.SYBR2GreenIRT2PCR[J].,2006,37(11),118921193.[11]BrownIH.Advancesinmoleculardiagnosticsforavianinflu2enza[J].DevelopmentsinBiological,2006,124:93297.[12],,.NASBA[J].,2005,25(11):91294.[13]LauLT,BanksJ,AherneR,etal.Nucleicacidsequence2basedamplificationmethodstodetectavianinfluenzavirus[J].Bio2chemBiophResCommun,2004,313(2):3362342.[14]CollinsRA,KoLS,FungKY,etal.Rapidandsensitivede2tectionofavianinfluenzavirussubtypeH7usingNASBA[J].BiochemBiophyResCommun,2003,300:5072515.[15]LiJ,ChenS,EvansDH.Typingandsubtypinginfluenzavir2ususingDNAmicroarraysandmultipleexreversetranscriptasePCR[J].JClinMicrobiol,2001,39(2):6962704.[16]KesslerN,FerrarisO,PalmerK,etal.UseoftheDNAFlow2ThruChip,athree2dimensionalbiochip,fortypingandsubtypingofinfluenzavirus[J].JClinMicrobiol,2004,(42):217322185.[17]TownsendMB,DawsonED,MehlmannM,etal.Experimen2talevaluationoftheFluChipdiagnosticmicroarrayforinflu2enzavirussurveillance[J].JClinMicrobiol,2006,(44):286322871.[18],,,.DNAAIVcDNA[J].,2005,38(2):3942398.[19]DawsonED,MooreCL,DankbarDM,etal.IdentificationofA/H5N1influenzavirususingasinglegendiagnosticmicroar2ray[J].AnalyticalChemistry,2007,79(1):3782384.[20].[D].:,2005:68272.[21]SenguptaS,OnoderaK,LaiA,etal.Moleculardetectionandidentificationofinfluenzavirusesbyoligonucleotidemicroar2rayhybridization[J].JClinMicrobiol,2003,(41):454224550.ProgressonAvianInfluenzaGeneDiagnosisTechnologyTIANLi2na1,2,WANGXiu2rong2,YANGZhong2ping2,SHILin2,TAOQi2meng2,CHENHua2lan2(1.CollegeofAnimalMedicine,NortheastAgriculturalUniversity,Harbin,Heilongjiang,150030,China;2.NationalAvianInfluenzaReferenceLaboratory,AnimalInfluenzaLaboratoryoftheMinistryofAgriculture,NationalKeyLaboratoryofVeterinaryResearchInstituteofChineseAcademyofAgricultureSciences,Harbin,Heilongjiang,150001,China)Abstract:Avianinfluenzaiscausedbytheavianinfluenzavirusandisahighlycontagiousdiseaseofhumansandanimals,notonlycausedpoultryindustryhugeeconomiclosses,butalsocausedhumansecuritypres2enceinpublichealththreat.Therefore,rapidandaccuratediagnosisofgenetechnologyforthepreventionandcontrolofavianinfluenzaisveryimportant.Atpresent,therearegeneticdiagnosissuchasRT2PCRtechnique,fluorescencequantitativeRT2PCRtechnology,relianceonthenucleicacidamplificationtech2nology(NASBA)andthenewmicroarraytechnology.Thepaperreviewedthebasicprinciplesofthesemethods,testingandapplicationoftheirmerits,withaviewtoeffectivelycontroltheavianinfluenzapan2demicoutbreakandtoprovideeffectivetechnicalsupport.Keywords:Avianinfluenzavirus;RT2PCR;NASBA;microarray262008298(180)©1994-2008ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.