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(,250012)Email:zhiyu.wang@sdu.edu.cn:E1RVELISAE1rubellavirusRVELISA90JINGMEIRECIRECIRV67.11%71.43%67.78%50%78.57%54.44%84.71%71.43%82.22%P0.05RECIP0.05RECIP0.05P0.05P0.05RVE1ELISA:ELISA1rubellavirus,RVRNACE1E2335842kDE1E111391541791992392262773894122132392582771RVRV2RVRVRV200304220182001BB1CAA7:21:41:81:161:501:1001:2001:400TMBAAP/N=AAAA−−P/NA2.5.248RECI2.5.3P/Nt2.5.4(1)1:10023.21.5.11:4P/N17.2831:10043.32P/NP0.051ELISA051015202530354045501:11:21:41:81:161Fig1.DilutedtitersofrecombinantproteinsP/NPatient/NormalValue1:501:1001:2001:40000.010.020.031:501:1001:2001:40021:8Fig2Dilutedtitersofnegtiveserum(recombinantantigen1:8)A(450nm)AValue(450nm):11:21:41:81:163Fig3DilutedtitersofRVculturemediumP/NPatient/NormalValue1:501:1001:2001:40000.010.020.030.040.050.061:501:1001:2001:400A(450nm)AValue(450nm)414Fig4Dilutedtitersofnegativeserum(virusantigen1:4)(P0.0534RECIP0.052(P0.055(P0.05626446812102276149023.06P0.05351455251035761490213.79P0.05438341381149761490228.20P0.05://=1.8512P0.05RV51LA.Mitchell,D.Decarie,AJ.Tingle,etal.IdentificationofimmunoreactiveregionsofrubellavirusE1andE2envelopeproteinsbyusingsyntheticpeptides.VirusResearch,1993,29:33-572E.Miller,JE.Cradock-WatsonandTM.Pollock.Consequencesofconfirmedmaternalrubellaatsuccessivestagesofpregnancy.Lancet,1982,2:781-7843G.Dominguez,C.Wang,TK.Frey,etal.SequenceofthegenomeRNAofrubellavirus:evidenceforgeneticrearrangementduringtogavirusevolution.Virology,1990,177:225-2384,,ELISA,,1987,25:44-465S.Michel,L.Christer,S.Aimo,etal.Detectionofrubellavirus-specificimmunoglobulinMantibodieswithabaculovirus-expressedE1protein.ClinicalandDiagnosticLaboratoryImmunology,1996,3:216-218(RV)antibodyusingyeastexpressionproductsascoatingantigenandtoimproveitssensitivityandspecificity.TheyeastexpressionproductsandRVculturemediumwereusedascoatingantigensrespectivelytoassay90sera.ThesameserawerealsoassayedbyJINGMEIkitandGermanyRECIkit.TheresultsofGermanykit,asagoldenstandard,werecomparedwiththoseoftheotherthreemethodsinspecificity,sensitivityandaccordancerate.Andthestatisticaltestwasusedtocomparethedifferencesamongthesemethods,andalsotoevaluatewhichismoreusefulforcoatingantigen,recombinantantigenorRVculturemedium.ComparedwithGermanyRECIkit,thesensitivityspecificity,andaccordancerateofrecombinantproteinwere67.11%,71.43%and67.78%,respectively,andthoseofRVculturemediumwere50%78.57%and54.44%,respectively.AndthoseofJINGMEIkitwere84.71%,71.43%and82.22%,respectively.Theresultsofrepeatedexperimentofrecombinantproteinshowedthattherewasnostatisticalsignificancebetweentwoexperiments(P0.05).ThedifferenceofthetwopositiveratesbetweenRECIkitandrecombinantantigenwassignificant.ThedifferenceofthetwopositiveratesbetweenRECIkitandRVculturemediumwassignificant(P0.05)Therewasnostatisticsignificance(P0.05)betweenthedifferencesofthepositiveratesofRECIkitandJINGMEIkit,buttherewasbetweenthoseofrecombinantantigenandJINGMEIkit(P0.05)andbetweenthoseoftherecombinantantigenandRVculturemedium(P0.05).thisresultsshowsthatascoatingantigen,therecombinantE1ProteinisbetterthanRVculturemediuminELISAmethodfordetectionofRVantibody.Keywordsrubellaviru,yeastexpression,glycoproteins,ELISA