质粒提取原理及步骤

Forpersonaluseonlyinstudyandresearch;notforcommercialuseDNA31.2.3.DNA()pUCLBDNA(pBR322)pBRg/ml)DNADNA1)(15kb)EDTASDS2)EDTADNADNADNA3)(HB101)DNADNAHB101TG14)A(endA+HB101)A()DNA())DNADNADNA()DNADNADNADNADNADNADNA()DNADNADNADNADNA2ml15ml301.5ml412000g304100l:50mmol/L25mmol/LTrisCl(pH8.0)10mmol/LEDTA(pH8.0)100ml,10lbf/in2(6.895104Pa)154200l0.2mol/LNaOH10mol/L1%SDS150l5mol/60ml11.5ml28.5ml3mol/L5mol/L10412000g5412000gDNADNA412000g1ml70%4DNA810i.f3pUC35iiDNA1lDNAl1l1012DNA20iii.100ml350lSTETSTET0.1mol/LNaCL10mmol/LTris.Cl(pH8.0)1mmol/LEDTA(pH8.0)5%TritonX-10025l[10mg/ml,10mmol/LTris.Cl(pH8.0)]3pH8.04012000g1040l5mol/LpH5.2420l5412000g51ml70412000g108251150lDNARNA20g/mlTE(pH8.0)-20endA+HB101DNAMg2+VDNADNADNADNApMBlColEl500ml25mgDNA30mlDNA6000.6)500mlTerrific3725ml3725300/OD6000.42.5ml34mg/ml170g/mlpBR322pUC37300/12-1644000/15100mlSTESTE0.1mol/LNaCl10mmol/LTris.Cl(pH8.0)1mmol/LEDTA(pH8.0)500ml[3]10ml18ml:50mmol/L25mmol/LTris.Cl(pH8.0)10mmol/LEDTA(pH8.0)10lbf/in2(6.895x104Pa)151ml(2ml)[10mg/ml,10mmol/LTris.Cl(pH8.0)]pH8.020ml(40ml):0.2mol/LNaOH(10mol/L1SDS5-1015nl(20ml):5mol/L60ml11.5ml28.5mlmol/L5mol/L100DNARNASDS44000/155000/20[]250ml0.610500/154703mlTE(pH8.0)10]15mlorex3ml5mol/LLiCl10000/10LiClRNA30mlCorexSorvallSS3410000/1070500lDNARNA(20g/mlTEpH8.030500l13w/v)PEG80001.6mol/LNaCl,12000g400lTE(pH8.0)DNA100l10mol/L1ml10412000g5200l412000g210500lTEpH8.01:100[TEpH8.0)]OD260DNA1OD26050gDNA/mlDNA20,,Forpersonaluseonlyinstudyandresearch;notforcommercialuse.Nurfrdenpers?nlichenfrStudien,Forschung,zukommerziellenZweckenverwendetwerden.Pourl'tudeetlarechercheuniquementdesfinspersonnelles;pasdesfinscommerciales.Forpersonaluseonlyinstudyandresearch;notforcommercialuse