辣根过氧化物酶的结构与作用机制

2005251CHEMISTRYOFLIFE2005,25(1),HBVpsxcpreS,DR1DR2HepG2,shRNA,hbv,HBVDNARNAYing[14]HBVcRNA(siRNA),YMDDHBVHepAD38HepAD79,DNA,Hamasaki[15]SapHBVDNAHuH7HepG2,HBV;csiRNA,2HBeAg45,HBsAg,3.5kbmRNA,2.4kb/2.1kbmRNA,McCaffrey[16]hbvshRNA,4HBV:(1)HBsAg;(2)hbvRNA;(3)HBVDNA;(4)HBcAgGiladi[17]ssiRNAhbvBALB/c,GiladisiRNA,siRNA,,,,,,,[1]ZhangLetal.Bioelectrochemistry,2004,63(1-2):369373[2]GregoriadisGetal.Vaccine,2002,20:B1B9[3]ChenLetal.IntImmunopharmacol,2004,4(3):403409[4]ShaoHJetal.ActaVirol,2003,47(4):217221[5]KwissaMetal.JMolMed,2003,81(2):91101[6].,2002,29(4):576582[7]WooPCetal.Vaccine,2001,19:29452954[8]DingCLetal.WorldJGastroenterol,2003,9(7):15121515[9]HoeprichSetal.GeneTher,2003,10(15):12581267[10]PanWHetal.MolTher,2004,9(4):596606[11]MorrisseyDVetal.JViralHepat,2002,9:411418[12].,2003,23(2):112115[13]YingCetal.BiochemBiophysResCommun,2003:398404[14]YingCetal.BiochemBiophysResCommun,2003(309):482484[15]HamasakiKetal.FEBSLett,2003(543):5154[16]McCaffreyAPetal.NatBiotechnol,2003,21(6):639644[17]GiladiHetal.MolTher,2003,8(5):769776:100021336(2005)0120033203(,310014):,,:;;:Q55:2004211226:(1980),,,E2mail:[e-mail];(1968),,,,;(1980),,(horseradish),,,H2O2,:,(horseradishperoxidase),33©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.2005251CHEMISTRYOFLIFE2005,25(1)MiniReviews,,C(HRPC)HRPC,HRPC1990,Smith[1]HRPC,X;1997,Gajhede[2]HRPC;HRPHRP,(Arabidopsisthaliana),,73,,[3,4]1.HRP1HRPC[6]1.1C308,Welinder[5]N,C4,1191444997301177209;Asp99Arg1239,8,775%80%,,GlcNAcHRPC,18%22%[6]HRPC:()His170N,His170(1);,H2O2,CO,Arg38His42,,7,,AspSerThr,HRPC[7],1.2HRPC1997[2],HRPC,HRPC;GajhedeHRPC2,,,,1.3HRP,HRP,HRP32(FF),Cys177Cys209,FFF43©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.2005251CHEMISTRYOFLIFE2005,25(1),HRP;[2]2.HRP2.1HRP,AH+H2O2A+2H2O2HRPC[2];;;AH,A,,,HRP,HRP,:,,,,:,,:HRP[8]2.2Dunford[9]HRP,HRPC3(ferulate),3,,H2O2Fe()(HRP),HRP,Fe()HRP(HRP0),HRP0H2O22Fe(),,HRP,Fe()HRPHRPHRP,+1V,HRPH2O2,,HRP,HRPHRPFe()Fe()[10]3HRPC[9]2.3HRPC,,1,C182C202OrtizdeMontellano[11]HRPCHRPCH2O2,,C182C202,,HRPP450,HRPP450,:HRPHRPFe(),HRPCHRPC53©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.2005251CHEMISTRYOFLIFE2005,25(1)MiniReviewsHRPC,HRPCPhe41His42,H2O2,HRPC112HRPC,,33HRPCArg38NH2,HRPCPro139();Phe68Gly69Pro139Ala140Phe142Phe179;C20H;C20H[12][1]SmithATetal.JBiolChem,1990,265(22):1333513343[2]GajhedeMetal.NatStructBiol,1997,4(12):10321038[3]TognolliMetal.Gene,2002,288(122):129138[4]WelinderKGetal.EurJBiochem,2002,269(24):60636081[5]WelinderKG.FEBSLett,1976,72(1):1923[6]YangBYetal.CarbohydrRes,1996,287(2):203212[7]HaschkeRHetal.BiochemBiophysResCommun,1978,80(4):10391042[8]KawaokaAetal.AnnNYAcadSci,1994,721:248249[9]DunfordHB.In:EverseJetal.eds.HorseradishPeroxidase:StructureandKineticProperties,BocaRatonFlorida:CRCPress,1991,124[10]BerglundGIetal.Nature,2002,417(6887):463468[11]OrtizdeMontellanoPR.AnnuRevPharmacolToxicol,1992,32:89107[12]HenriksenAetal.JBiolChem,1999,274(49):3500535011:100021336(2005)0120036203PrP1,21113(1BSE,266032;2,411201;3,330045):PrP106126PrP118135PrPC106126118135,PrPSc,PrP106126PrP118135PrPSc:PrP106126;PrP118135;:Q51:2004211230(No.30270981)948(No.982070):(1979),,,,E2mail:[e-mail];(1965),,,,,E2mail:[e-mail](transmissiblespongiformen2cephalopathies,TSE),(prion)(Creutzfeldt2Jakobdisease,CJD)Gerstmann2Straussler2Scheinker(bovinespongiformencephalopathy)TSE[1]PrPCPrPScPrPC,,PrPCPrPSc,2730kD(PrP2730),PrP2730,,PrPScTSE,PrPSc,PrPSc63©1995-2005TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.