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:20030711:(1962),,,;(1962),,,1,2(1.,250013;2.,250100):MB1,,SephadexG-100,SephadexG-75SephadexG-50,SephadexG-75,,6.94Umg-1200.9Umg-1,28.9,8.6%:;;;;:Q814.1:A:10082816(2003)06008803,,,,,,,,,DNA,[1],,,;,,,,,[2],MB1,411.11.1.1MB1[3]4,8000rpm,15,1.1.2:10,000NMWL,10,000NMWL:SephadexG-100;SephadexG-75;SephadexG-50;(AMERSHAMPHARMACIABIOTECH)1.1.3(Amico),,DYY-2();DYY-m24D();50cm2.6mm;8823A();;;;1.21.2.1[4]1.2.2Folin-[5],(BSA)1.2.31.2.3.1:4,8000rpm,15,,102g/mL,14.7mg/ml1.2.3.2:,25%,4,10000rpm,10,,,0.05mol/LPh6.0,,,1.2.3.3SDS-SPGE,12%,4%,0.25%(W/V)20036100R-2501.2.3.4SephadexG-100,SephadexG-75SephadexG-50,,,(50cm2.6mm),0.05mol/LpH6.0,4.0mL,7mL/h,0.2A,20,(2.4mL),CMCCMC,2.4mL,SephadexG-100,SephadexG-75SephadexG-50,1231SephadexG-100Fig.1GelfiltrationchromatogramofSephadexG-1002SephadexG-75Fig.2GelfiltrationchromatogramofSephadexG-753SephadexG-50Fig.3GelfiltrationchromatogramofSephadexG-50(1)1,SephadexG-100,CMC,(2)2,SephadexG-75,,,,SDS-PAGE,,,(3)3,SephadexG-50,,,;,,,SephadexG-100SephadexG-75SephadexG-50,SephadexG-75;SephadexG-75,11SephadexG-75(U)(mg/mL)(Umg-1)(fold)ss10200014.76.941100SephadexG-7588402.2200.928.98.63(1)SephadexG-100SephadexG-75SephadexG-50,SephadexG-75,,28.9(2),,,,,,,,;,,(3),8%,,4,:[1].[M].:,1999,168.[2]D.R.Marshaketal.StrategiesforProteinPurificationandCharacter2ization:ALabroratoryCourseManual.ColdSpringHarborLaboratoryPress,1996.[3],.[J].,2003,27(5):42-45.[4]HorikoshiK,NakaoM,KuronoY,etal.Cellulaseofanalkalophilic98100Bacillusstrainisolatedfromsoil.CanJ.Microbiol,1984,30:774-779.[5].[M].:,1994,168-170.SeparationandPurificationofCellulasebyColumnChromatographyoftheGelFiltrationWangBin1,YuanFangyao2(1.Shandongeducationalcollege250013;2.ShandongAcademyofAgriculturalSciences250100)Abstract:ThecrudecellulaseextractionfromamarinebacteriumMB1wasseparatedandpurifiedbytheammoniumsulfateprecipitation,dialysisandcolumnchromatographyofSephadexG-100SephadexG-75andSephadexG-50.TheeffectoftheSephadexG-75wasthebestandonecellobiohydrolase(CBH)waspurifiedtoelectrophoretichomogeneitybySephadexG-75columnchromatography.Thespecificactivityoftheenzymewasraisedfrom6.94Umg-1to200.9Umg-1,whichwas28.9timesthatoftheculturesupernatantwithayieldof8.9%.Keywords:cellulase;cellobiohydrolase;gelfiltration;columnchromatography;separationandpurification(87)StudyandApplicationofCellVariantsinPlantYuHuimin,ShiZhu(DepartmentofBiology,ShandongEducationCollege,Jinan250013,China)Abstract:Theexpetedadversity-resistanceofplantcellcanbeselectedbythetissuecultureinviroundercertainstressfactors.Thispa2permainlyanalysedandreviewedtheselectionandapplicationofallkindsofdirectivelyselectedcellvariantsreportedinrecentyears.Par2ticularly,thefocusofthispaperwasputonthecellvariantswhichresistcertainstresssuchassalt,drought,lowtemperatureanddisease.Thevariantsresistanttoaminoacidsandtheiranlogueswerealsowithinthisscope.Keywords:variant;tissueculture;selection09:20036