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120h。120h~168h108h。120h。4、、pH、12h~15h20h~25h、1∶5、pH7、28℃、120h。44.9193mg/L。[1]PaivaSRussellR.Betacaroteneandothercarotenoidsasantioxidants[J].JAmColl,NutritionResearch,1999,18:426-433[2]KsenijaMarkovic',MirjanaHrusˇkar,NadaVahcˇic.LycopencontentoftomatoproductsandtheircontributiontothelycopeneintakeofCarotenes[J].NutritionResearch,2006,26:556-560[3]JannatM.Rolda'n-Gutie'rrezandMaríaDoloresLuquedeCastro.Lycopene:Theneedforbettermethodsforcharacterizationanddetermination[J].TrendsinAnalyticalChemistry,2007,26(2):163-170[4],.[J].2000,26(2)75-79[5],,,.[J].2007,14(4):47-50[6],,.[J].2008,12(4):14-162010-10-07[1][2]。、[3-4]。111121.4710032.030032。、30℃pH7.0。ScreeningofLipaseProducingStrainsandOptimizationoftheLipaseProductionLIXin-ling1,SUNXiao-fei1,MENGNan1,BUMei-ling1LIUJin21.FoodandBioengineeringDepartment,HenanUniversityofScienceandTechnology,Luoyang471003,Henan,China;2.TsingtaoBreweryCo.,Ltd.,Taiyuan030032,Shanxi,ChinaAbstract:Thisthesisreportsthatalipase-produingbacterialstrainwasisolatedfromtheoilysoilsamplesandwasidentifiedaspseudomonas.Thelipasefermentationconditionforthestrainwasoptimizedbystudyingoneffectofnitrogensources,carbonsourcesandotherinfluencefactors.Theoptimumcarbonandnitrogensourcewasdextrinandammoniumsulfate.TheoptimumpHwas7.0,andtheoptimumtemperatureforfermentationwas30℃.Keywords:lipase;pseudomonas;optimallipase-producingcondition1982—。!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!20116326FoodResearchAndDevelopment1191Fig.1Effectofcarbonsourceonlipasesproduction/U/mL2Fig.2Effectofnitrogensourceonlipasesproduction/U/mL。11.1、45。1.2%0.02,Na2HPO40.35,KH2PO40.15,MgSO4·7H2O0.05NaCl0.051.0pH7.0%0.20.21.01.01.5pH7.0%2.0NH42SO40.5K2HPO40.11.0MgSO4·7H2O0.052.5pH7.0%2.00.51.0NH42SO40.1MgSO4·7H2O0.05K2HPO40.1pH。1.32g20mL3mL30mL32℃、200r/min4d300μL3。。。1.4[5]NaOH40℃、pH7.5,1μmol1U。。1.5、《》[6]。22.174。4。2.2LBLuria-Bertani。。4。2.32.3.1、、6.0U/mL1。2.3.2、、、2%、、。15U/mL2。2.3.3pHpHpH7.012.0U/mLpH93。2.3.430℃30℃9.9U/mL4。1451203pHFig.3EffectsofdifferentinitialpHonenzymeproductionpH/U/mL4Fig.4Effectoftemperatureonlipasesproduction/℃/U/mL3。。。30℃pH7.0。[1]JaegerKEDijkstraBWReetzMT.Bacterialbiocatalysts:molecularbiology,three-dimensionalstructuresandbiotechnologicalapplicationsoflipases[J].AnnualReviewofMicrobiology,1999,53:315-351[2]HasanFShahAAHameedA.Industrialapplicationsofmicrobiallipases[J].EnzymeandMicrobialTechnology,200639(2)235-251[3]KrishnakantS,DattaM.EstersynthesisbylipaseimmobilizedonsilicaandmicroemulasionbasedorganogelsMBGs[J].ProcessBiochemistry,2001,36:607[4]HemachanderC,PuvanakrishnanR.Lipasefromralstoniapickettiiasadditiveinlaundrydetergentformulations[J].ProcessBiochemistry,2000,35:809[5],,,.[M].:,1994:3-11[6]RE,NE.[M].8.:,1984:274-3002010-10-10。23、、TBARS、pH。37±1℃220±1℃30℃~4℃4。35.52%。[1],,.[J].,1992(1):6-8[2],,.[J].,2009,30(2):149-153[3],,,.[J].,2005,33(7):60-61[4],,.[J].,2009,18(3):39-41[5]pH[J]..2000(5):27[6]SinnhuberRO,YuTC.The2-thiobarbituricacidreaction,anobjectivemeasureoftheoxidativedeteriorationoccurringinfatsandoils[J].JournalofJapanOilChemists'Society,1977,25:259-267[7],.[J].,2008,29(8):5-6[8],,.[J].,2002(12):8-102011-02-15!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!120145