Western-blot-胶配方

分离胶12%分离胶15%浓缩胶5%5ml10ml5ml10ml2ml4mlDDW1.6mL3.3mL1.1mL2.3mL1.4mL2.7mL30%聚丙烯酰胺2.0mL4.0mL2.5mL5.0mL330uL670uLTris-HCL1.3mL（3M,pH8.8）2.5mL1.3mL（1.5M,pH8.8）2.53mL250uL(pH6.8)500uL10%SDS50uL100uL50uL100uL20uL40uL10%aAP50uL100uL50uL100uL20uL40uLTEMED2uL4uL2uL4uL2uL4uL分离胶12%分离胶15%浓缩胶5%5ml10ml5ml10ml2ml4mlDDW1.6mL3.3mL1.1mL2.3mL1.4mL2.7mL30%聚丙烯酰胺2.0mL4.0mL2.5mL5.0mL330uL670uLTris-HCL1.3mL（3M,pH8.8）2.5mL1.3mL（1.5M,pH8.8）2.53mL250uL(pH6.8)500uL10%SDS50uL100uL50uL100uL20uL40uL10%aAP50uL100uL50uL100uL20uL40uLTEMED2uL4uL2uL4uL2uL4uL