数字pcr与同类产品的比较

RainDanceTechnologies™CompanyConfidentialSuperiorValuePropositionforRainDrop™DigitalPCRPartitions10,000,00020,00020,0003,072Multiplexing10-plex2-plex2-plex2-plexDynamicrange5-6logs3logs3logs3logsSensitivity1in250,0001in6,000?1in1,000Closed-tubeSystemYesNoYesYesWorkflowProprietaryReagentsNoYesYesYesThroughput/Shift2419218?12Instrumentprice$225,000$19,000$90,000$170,000Pricepersample$30$3$15-$35$150QuantStudioOpenArrayQX100QuantStudio3DRainDanceTechnologies™CompanyConfidentialRainDanceTechnologiesRainDanceTechnologiesRainDanceTechnologiesQX100™DropletDigital™PCRSystem2LoadsamplesandoilintocartridgeAttachsealinggasketPlacecartridgeintoGeneratorTransfersamplesintoplatePipettedropletsfromcartridgeRemovecartridgefromGeneratorandremovesealinggasketSealplateRemoveplatefromcyclerLoadintoReaderandrunAnalyzedatausingQuantaSoftTotaltime~6hours36minsRainDanceTechnologies™CompanyConfidentialRainDanceTechnologiesRainDanceTechnologiesRainDanceTechnologiesSellingAgainstBioRadWorkflow3CompetitorRainDropSolutionTakeHome20,000dropletsgeneratedUpto10,000,000dropletsgenerated20Kdropletsdon’tgiveyousinglemoleculecounting–relianceonPoissonstatisticstogenerateresults.Limitedtoduplexing.CrosscontaminationofsamplesfromreusedsamplingprobeandflowpathSingleusedisposableflowpathYouworkhardtogetthehighestqualityresults…don’triskthissample’sanswerwiththeprioroneVacuumdeliveryofsamplescanproduceinconsistentlysizeddropletsMonitordropletsizeduringgenerationandadjustpressureasnecessarytomaintainconsistentsizeConsistencyinprocessingisthebasisforconsistencyinresultsThermocyclingisbasedon96wellplatewhichremainsopenuntillastsamplesareloadedClosedtubedesigneliminatesthepotentialforcontaminationandspills.8samplestripdesignreduceswasteandspeedstimetofirstresultContaminationcontrolisimportantindigitalPCRRequiresproprietarymastermixWorkswithseveralmastermixesLeadingedgesciencedemandsflexibilityRainDanceTechnologies™CompanyConfidentialRainDanceTechnologiesRainDanceTechnologiesRainDanceTechnologiesAppliedBiosystemsQuantStudio™12KFlexbyLifeTechnologies4Loadsamples,MM,andassayinto384wellplateforAccuFill,sealwithfoil,thencutPlace384wellplate,tips,andarrayinAccuFillPlaceinplatesealerandapplylid–90seconds!PlaceinholderScrewtosealFillwithimmersionfluidMergesampleinfooninstrumentAnalyzedatausingDigitalSuiteTotaltime~4.5hoursexcludinganalysisLoadintoinstrumentandrun~4hoursRainDanceTechnologies™CompanyConfidentialRainDanceTechnologiesRainDanceTechnologiesRainDanceTechnologiesSellingAgainstQuantStudio12KFlexOpenArray5CompetitorRainDropSolutionTakeHome3072maximumpartitionsUpto10,000,000dropletsgenerated3072partitionsdon’tgiveyousinglemoleculecounting–relianceonPoissonstatisticstogenerateresultsUpto48pipettingstepspersampleOnepipettingsteppersampleMorepipetting,moretime,moremoney,moreerrorCapillaryfillingoftipsandarraycanfailtofillpartitionsMonitordropletsizeduringgenerationandadjustpressureasnecessarytomaintainconsistentsizeConsistencyinprocessingisthebasisforconsistencyinresultsOnceloadedarraysarefragile–samplecanevaporate,getflushedoutofpartitions,orgetjarredfree,andbreakdownifnotrunimmediatelySamplesareinsealedPCRtubes,cannotevaporateorspill,andcanbestoredupto3daysifnecessaryFlexibletimingwithnorushfitsbetterintodailylabroutinesRequiresproprietarymastermixWorkswithseveralmastermixesLeadingedgesciencedemandsflexibilityRainDanceTechnologies™CompanyConfidentialRainDanceTechnologiesRainDanceTechnologiesRainDanceTechnologiesQuantStudio™3DDigitalPCRSystem6Mixsample,assay,mastermix,andpipetontochipPlaceontoloader,usesqueegeetodistributeacrosschipPourimmersionfluidonchip,snaplidintoplateTransferdatatocomputerInsertchipintoinstrumenttoread,~2minPlaceinthermalcycler~3.5hoursSetROX,FAM,andVICbaselinesFuturesoftwareforRareAlleleandAbsoluteQuantitationTotaltime~4.5hoursexcludinganalysisRainDanceTechnologies™CompanyConfidentialRainDanceTechnologiesRainDanceTechnologiesRainDanceTechnologiesSellingAgainstQuantStudio3DDigitalPCRsystem7CompetitorRainDropSolutionTakeHomeUpto20,000partitionsUpto10,000,000dropletsgenerated20kpartitionsdon’tgiveyousinglemoleculecounting–relianceonPoissonstatisticstogenerateresultsPipetting/squeegeeloadingofsampledifficult,requiresloader,usescapillaryaction-Onepipettingsteppersample-ClosedlooppressurecontrolallowsRainDropSourcetodropletizetheentiresampleineachlanesimultaneouslyMorepipetting,moretime,moremoney,moreerror.CapillaryfillingcanresultininconsistentsamplevolumesPouringimmersionfluidandsnappinglidcanflushsamplesoutofpartitionsSamplesareinsealedPCRtubes,cannotevaporateorspill,andcanbestoredupto3daysifnecessaryConsistencyinprocessingisthebasisforconsistencyinresultsMustsitatinstrumenttofeedchipsineverycoupleminutesWalkawayworkflowwithminimalhandsontimeAt20Kperchip3Dwouldrequire50chipstoget1,000,000datapoints,whichwouldtakenearly2hoursofhandsontimetoreadRequiresproprietarymastermixWorkswithseveralmastermixesLeadingedgesciencedemandsflexibilityRainDanceTechnologies™CompanyConfidentialInosticsBEAMingServiceRainDanceTechnologies™CompanyConfi