pGEX质粒图谱

pGEX-1λT(27-4805-01)pGEX-6P-1(27-4597-01)EcoRISmaISalIXhoINotIBamHIPreScission™ProteaseLeuGluValLeuPheGlnGlyProLeuGlySerProGluPheProGlyArgLeuGluArgProHisCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGGAATTCCCGGGTCGACTCGAGCGGCCGCAT↓pGEX-6P-2(27-4598-01)BamHIPreScission™ProteaseLeuGluValLeuPheGlnGlyProLeuGlySerProGlyIleProGlySerThrArgAlaAlaAlaSerCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCAGGAATTCCCGGGTCGACTCGAGCGGCCGCATCG↓EcoRISmaISalIXhoINotIpGEX-6P-3(27-4599-01)BamHIPreScission™ProteaseLeuGluValLeuPheGlnGlyProLeuGlySerProAsnSerArgValAspSerSerGlyArgCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCGAATTCCCGGGTCGACTCGAGCGGCCGC↓EcoRISmaISalIXhoINotIBamHIEcoRISmaISalIXhoINotIBamHIEcoRISmaISalIXhoINotIBamHIEcoRISmaISalIXhoINotIBamHIEcoRISmaISalIXhoINotIBamHIEcoRISmaISalIXhoINotIBamHIEcoRISmaISalIXhoINotIEcoRICTGGTTCCGCGTGGATCCCCGGAATTCATCGTGACTGACTGACGABamHILeuValProArgGlySerProGluPheIleValThrAspThrombinStopcodons↓pGEX~4900bppBR322oriBalIBspMIPtacglutathioneS-transferaseAmprlacIqNarIEcoRVBssHIIBstEIIMluIApaITth111IAatIIPstIp4.5AlwNIpSj10Bam7Stop7∆pGEX-4T-2(27-4581-01)pGEX-5X-1(27-4584-01)pGEX-5X-2(27-4585-01)pGEX-5X-3(27-4586-01)pGEX-4T-1(27-4580-01)pGEX-4T-3(27-4583-01)pGEX-3X(27-4803-01)pGEX-2TK(27-4587-01)LeuValProArgGlySerProGlyIleProGlySerThrArgAlaAlaAlaSerCTGGTTCCGCGTGGATCCCCAGGAATTCCCGGGTCGACTCGAGCGGCCGCATCGTGAStopcodonIleGluGlyArgGlyIleProGluPheProGlyArgLeuGluArgProHisArgAspATCGAAGGTCGTGGGATCCCCGAATTCCCGGGTCGACTCGAGCGGCCGCATCGTGACTGAStopcodonsIleGluGlyArgGlyIleProGlyIleProGlySerThrArgAlaAlaAlaSerATCGAAGGTCGTGGGATCCCCGGAATTCCCGGGTCGACTCGAGCGGCCGCATCGTGAStopcodonIleGluGlyArgGlyIleProArgAsnSerArgValAspSerSerGlyArgIleValThrAspATCGAAGGTCGTGGGATCCCCAGGAATTCCCGGGTCGACTCGAGCGGCCGCATCGTGACTGACTGAStopcodonsLeuValProArgGlySerProGluPheProGlyArgLeuGluArgProHisArgAspCTGGTTCCGCGTGGATCCCCGGAATTCCCGGGTCGACTCGAGCGGCCGCATCGTGACTGAStopcodonsLeuValProArgGlySerProAsnSerArgValAspSerSerGlyArgIleValThrAspCTGGTTCCGCGTGGATCCCCGAATTCCCGGGTCGACTCGAGCGGCCGCATCGTGACTGACTGAStopcodonsATCGAAGGTCGTGGGATCCCCGGGAATTCATCGTGACTGACTGACIleGluGlyArgGlyIleProGlyAsnSerSerStopcodonsLeuValProArgGlySerArgArgAlaSerValKinaseCTGGTTCCGCGTGGATCTCGTCGTGCATCTGTTGGATCCCCGGGAATTCATCGTGACTGAStopcodonsThrombin↓Thrombin↓Thrombin↓Thrombin↓FactorXa↓FactorXa↓FactorXa↓FactorXa↓EcoRIBamHISmaIEcoRIBamHISmaIpGEX-2T(27-4801-01)CTGGTTCCGCGTGGATCCCCGGGAATTCATCGTGACTGACTGACGLeuValProArgGlySerProGlyIleHisArgAspStopcodonsEcoRIThrombin↓BamHISmaI