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A91A45201.2.3.4.[H]5.0.561212.3.4.55.6.110101.A.B.C.D.2.A.B.C.D.3.ATPA.2B.4C.36D.384.A.B.C.D.25.A.B.C.D.6.A.100,30minB.180,10minC.121,2030minD.160,60min7.pHA.4.0B.4.0-6.0C.6.5-8.0D.8.08.strRA.B.C.D.9.A.B.C.D.10.A.B.C.D.110101.2.2%3.ED4.5.6.7.8.9.10.4281.(10)G+-G,-2.(6)3HHATPATP3.(6)4.61234120FF1F+F2F-FFF+3HfrHfrF+FF+F-4FHfrFFF1F+F-F+FF-F+2HfrF-HfrFDNAFF-FF-FHfrF-F-3FF-F4B452012,3:5.6121.22.10-51.53.24.35.1.56.__________21.2.3.4.5.6.11.A.B.C.D.2.A.B.C.D.53.EDA.DNAB.C.D.4.A.B.ATPC.D.5.A.B.C.D.6.A.B.C.D.7.A.B.C.D.8.A.DNAB.1050C.D.9.FA.B.C.D.10.A.B.C.D.110101.2.3.4.5.6.7.8.9.10.1.2.3.4.5.6.7.8.9.10.4281.662.83.F8F1F+F2F-F3HfrHfrF+F4FFF4.120T4T4(2)T4(4)T4T4(6)DNA-DNA--(6)(27C45201.2.:3.:DNA4.DNA5.0.55121.1.52.2.53.24.3.55.2.51.2.HMPED,TCAED3.4.5.110101.A.B.C.D.2.()A.B.C.D.3.()A.B.C.D.84.A.B.ATPC.D.5.A.B.C.D.6.1000ml()A.0B.1520C.27D.1007.F-F+A.B.C.D.8.HfrA.B.C.D.9.A.B.C.D.10.A.B.C.D.110101.2.3.4.EcoliK12K125.6.7.8.GC9.10.1.2.3.4.5.6.7.8.9.10.4281?61239261N-N-?-1423L-Ala-D-Glu-L-Lys-D-Ala310123454612312012263646