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。、。5、、14.54%、21.98%、33.14%210.4mg·kg-197.2mg·kg-1。、、1.60×105、3.09×105、7.08×1032.69×104、2.57×104、4.07×103。。。X53A1672-2043201806-1159-07doi:10.11654/jaes.2017-1549DegradationofpetroleumhydrocarbonsduringthebioremediationofcultivatedloessialsoilWUMan-li,ZHANGChen,QIYan-yun,YEXi-qiong,ZHUChang-chengSchoolofEnvironmentalandMunicipalEngineering,Xi′anUniversityofArchitectureandTechnology,KeyLaboratoryofNorthwestWaterResources,EnvironmentandEcology,MinistryofEducation,Xi′an710055,ChinaAbstractAmicrocosmstudywasconductedtoassesstheeffectsofbioremediationandlimitingfactorsonpetroleumhydrocarbondegrada－tionduringtheremediationofoil-contaminatedsoil.Thecontentsofpetroleumhydrocarbons,nitrogentotalN,nitrateN,andammo－niumN,availablephosphorus,andhydrocarbon-degradingmicroorganismsweremeasuredduringbioremediation.Thecorrelationsbe－tweenpetroleumhydrocarbondegradationandinfluentialfactorswereanalyzedusingSPSS19.0.Biostimulationwasmoreeffectivethanbioaugmentationinremovingpetroleumhydrocarbonsfromthecultivatedloessialsoilanddegraded14.54%,21.98%,and33.14%oftheto－talpetroleumhydrocarbonsTPH,alkanes,andpolycyclicaromatichydrocarbonsPAHsrespectivelywithinfiveweeks.TheammoniumNcontentofthesoildecreasedfrom210.4mg·kg-1to97.2mg·kg-1,andthenitrateNwasunaffected.ThepopulationsofTPH-,alkane-,PAH-degradersreached1.60×105,3.09×105,and7.08×103CFUg-1soil,respectively,inthebiostimulationtreatment,whichwasgreaterthanthoseinthecontroltreatment.CorrelationanalysisindicatedthatbioremediationwaslimitedbythecontentsofavailablephosphorusandammoniumN,whichwererelatedtoTPHandalkanedegradation.Keywordspetroleumcontaminatedcultivatedloessialsoil;bioaugmentation;biostimulation;ammoniumnitrogen;hydrogen-degradingpopu－lation;limitingfactor2017－11－082018－01－241974—。E-mail447005853@qq.com215771092015JM5163ProjectsupportedTheNationalNaturalScienceFoundationofChina21577109;TheNaturalScienceFoundationofShaanxiProvince,China2015JM51632018376:1159-116520186JournalofAgro-EnvironmentScience.[J].,2018,3761159-1165.WUMan-li,ZHANGChen,QIYan-yun,etal.Degradationofpetroleumhydrocarbonsduringthebioremediationofcultivatedloessialsoil[J].JournalofAgro-EnvironmentScience,2018,3761159-1165.710055376[1-2]。[3]10850~13488mg·kg-121~27、。。、[4-5]。BioaugmentationBiostimulation[6-7]。。[8-10]。H2O2、O2[11-13]。100/10/1[14-15]。、pH、、。[13]。Haghol－lahiA[16]、、270d70%23.5%。10%。。。。SPSS、。。11.1、、0.85mm、。、、、、、“”[17]-[18]GC-MS[19]。、、[20]。1。1.25g50mL150r·min-1、30℃2h。5mL100mLPBS7d。34℃、10000r·min-110min30mLPBS10min5。PBSOD1.0600nm。1.3BA800g1.210mLOD600=1.0108cfu·g-1。1Table1Physicalandbiochemicalpropertiesofthepetroleum-contaminatedsoil/mg·kg-118800-/mg·kg-113266GC-MS/mg·kg-13066GC-MS/%5.3/%0.39/g·kg-11.17-/mg·kg-10.84/mg·kg-112.39/mg·kg-111.77/MPN·g-1soil2.95×105/MPN·g-1soil2.95×105/MPN·g-1soil5.75×102116020186CKBABSa-b-c-P0.051Figure1Theconcentrationsofdifferentfractionalpetroleumhydrocarbonsincultivatedloessialsoil19.018.518.017.517.016.516.015.515.0/TPH/g·kg-1012345aAABAABABCABCABB14.013.012.011.010.09.0//g·kg-1012345bABCABCAABAABAAB3.53.02.52.01.51.0/PAHs/g·kg-1012345cABBABCAAAAAAAABBS800g1.2023gNH4NO30.5049gKH2PO4C∶N∶P=100∶10∶1。CK800gCK。35。2g、、、。1.4SPSS19.0、、。ANOVAP＜0.05、、、、、Pearson[21]。22.1、、1a、1b、1c。、、18800±115.4、13266±500.0、3066±333.3mg·kg-1。5CK、18033±233.3、13516±316.7、2416±50.6mg·kg-14.08±0.02%、-1.88±0.01%、21.23±0.07%BA、、17916±383.3、13016±183.3、2050±116.7mg·kg-14.70±0.03%、1.88±0.05%、33.14±0.16%BS、、16066±66.7、10350±183.3、2050±216.7mg·kg-114.54±0.01%、21.98±0.04%、33.14±0.13%。、。[1114]。。。[22-23]。[1024]。11613764003002001000//mg·kg-1012345bABBABBABBABBABB1.61.41.21.00.80.60.40.20//g·kg-1012345aABCABCABBABCABC。。[13]。[25]。。2.22a、2b、2c。12.4±0.05mg·kg-1。CK、BABS248.2±3.71mg·kg-134。。0.84±0.05mg·kg-1BA15.21±0.17mg·kg-1251.38±0.08mg·kg-1。BS210.4±4.32mg·kg-11171.6±1.68mg·kg-1。3597.2±0.43mg·kg-1。。。[11]、、。。。2.3311.8±0.14mg·kg-1CK12.3±0.15~15.6±0.50mg·kg-1BA22.2±1.20~26.2±0.15mg·kg-1BSKH2PO493.5±2.5mg·kg-14、584.1±1.53mg·kg-187.4±0.75mg·kg-1。a-b-c-2Figure2ThechangesofvariousofnitrogenindifferenttreatmentsCKBABS250200150100500//mg·kg-1012345cABBABBABBABBABB1162201868.38.28.18.07.97.87.77.6//lgCFU012345aABBABBABBABBAAA3Figure3Thechangesofavailablephosphorusindifferenttreatments[14-1525]。。。2.44、、1.78×108CFU·g-12.95×105、2.95×105、5.75×102MPN·g-1。CKBSBA1.10×107MPN·g-112.85×107MPN·g-122.96×105MPN·g-1。BS、24.30×107MPN·g-11.25×107MPN·g-121.60×105MPN·g-12.96×105MPN·g-115.75×102MPN·g-11.60×105MPN·g-157.08×103MPN·g-1。。。[13]、Wu[19-20]、[25]a-b-c-d-4Figure4ThecountsofhydrocarbondegradersindifferenttreatmentsCKBABS100806040200//mg·kg-1012345ABCABCABCABCABCCKBABS9876543/TPH/lgMPN012345ABCABCABCAABABBb9876543//lgMPN012345AABAABABCAABABBc65432/PAHs/lgMPN012345AAAABBABCABBABBd1163376。。。、。2.5BS、。SPSS19.0。2。BS、、、0.641、0.793、0.570、0.693、0.571、0.508、0.5520.543、。。。315、、14.54%、21.98%33.14%。。2。、、。3。[1],,.[J].,2009,165145-148.LIXiao-li,LIUGuo-bin,XUMing-xiang．FeaturesofoilpollutionofsoilandsurfacewaterinNorthemShaanxioilfield[J]．ResearchofSoilandWaterConservation,2009,165145-148．[2],,,．[J]．,2000,18462-64．HUANGTing-lin,XIEYue,WANGXiao-ch