荧光显微镜的功能配置及应用要点

:2004-05-2812(11,110001;21,110001)1002-2376(2004)09-0004-03TH773A,,,,;;;CCD,100,,FISH(GFP),CCD,1:,()()()():(),(1015s),,():,,,,,,,,(),,,,013(Excitation)(Emission),,,/,(320400nm),,2,/:,,,(365405449550600nm);(FITC,436490nm),;(Ca++)234MedicalEquipmentVol117,No19©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.()/:,/,/(),45(DichroicBeamsplitter)/,EX470/40EX480/20,470nm20nm480nm10nm,EM522/40EM500,522nm20nm500nm(),,,,1,13,,1),,,ND2)//,,,/,/,GFPFISH,,/(,Chroma,Omega)/11//(nm)(nm)DBS(nm)U330385420400DAPI,Hoechest3325833342,DNA360370455455360370420460400UFITC,UFITCB450480500505FITC,,PIDNARNA,450490515505460490515550505FITCTRITCTexasred,FITCG470490500520495GFPGFP510550590570,TRITC,PI,DNA5205505805653),UV,,:(1)(2),,,(3),(=NA/)(4),40,,(5)100(6)FURA2Ca++UV,UV4),,6V30W520049©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.(DIC),12V100W;(),DICCCD5),011%1%,,,6)CCDCCD,CCD,,CCD,CCD,(DarkCurrent)41)(1),(2)/,,(3),(4)(),,10m(5),,(6),(7),15min;,10min,,2)(1)510min,;,ND,,,(2),:,DICND/(3),CCD,(100X),,(4),(5),,UV,3)CCD(1),,,ISO200(24DIN)ISO400(27DIN),,,,,,(2)CCDCCD,(BackgroundSubtraction),BinningGainGammaCCD,,,ND,,,6MedicalEquipmentVol117,No19©1994-2007ChinaAcademicJournalElectronicPublishingHouse.Allrightsreserved.