DB61∕T 413-2007 饮料中D一苹果酸的测定-酶法

整理文档很辛苦,赏杯茶钱您下走!

免费阅读已结束,点击下载阅读编辑剩下 ...

阅读已结束,您可以下载文档离线阅读编辑

资源描述

DB611if~1*DB61/T413-2007tI2007-09-03~~2007-09-03~~DB611T413-2007*tiFftJ:f:ll¥J~t~A~~*,H1~HjL*~ft~J:f:l~A~~~~M®mA~~.~~~.m*acQ*~ft~~~N:J:f:l~A~~~~~®mA~~.~~~QI*~ft~~A:~*~'~~*'*WW'~~~Q*tjf,ftJktt~~;fpQIIIiIII!ii~!jII!•,~Jl;i~IIDB61fT413-2007tfItl1;:$:tift/fUJI!7l'if¢f~WlulE11A*4*D-:3jZ*mUfJ~iitl¥r1J~o*fiftttEmmTiX*4*D-1f:*~~itW!IJlEo2tJUiz31iJImjc14r:yux1LI=*i¥J~~jffiJi*ti~ttEi¥JiJ!)..trn'fiJj~J~;:$:t;j;ttEi¥J,}~oJ'LM:r:EBjtJji¥JiJ!J+1~ft~:,;ltPiQfr§J'Ji:tHi1.a.(~~~~~i¥J~~)~.rr~~~mmT*~ttE,~W,.a.M*~ttE~~~~i¥J*~~~M~EJ1~ffli3:~X11:i¥J1iifA&;:$:ofLfik~ffBWHfJt}rm~fll:,;ltiid'#rA&;:$:JffJ::rFf;:$:flFttEoEN12138:;J\.~*UJli~rtcpD-1j'i:~Il1tti¥Jl'if¢W!~1E.D-MDHD1j'Z*llil1i1i:lll?tI.~NAD:tj!JfiIi£~~~,*-A~1rllilNADHf3-':li!Iw:-~n~ut-'--':'i%tfllil~U~mIJ[~~lFJfl-f.fr.,if25·Ci¥Jfij\It~14:ftJ=5}~1Ui¥J~m~it.$tfftPX;1~moli¥J~~0A11&7tf1tiEi:C~r9tR~*JJthepes4-f5:z..~IJJ&~z:.~jl'imlIJ4JliHfD-1jL~~(D-~*~tik){tD1jL*~1i1i:~£~JW(D-MDH)Hi£i¥Jt~{5r.F,i!liJiNAD~f{:,7~~lP=mz..~tikolltmz..lliliiL0::liPEEffiJtfi¥J~{{tft?Hff.PX;jA]Iiil~1i1i:*11~.:~ftfWt0D-MDH,decarbJ) D-~*~1i1i:+NAD...p;jm1~~1i1i:+CO:c+NADH+HNADHi¥J,~,ffiD-1jL*~!Iii¥J,~,:!tit11:0IINADHi¥J~id:rntE334,340!&365nm4}{!\·r'i¥J1l'&7tJJ[{Ei:i¥J-'f:tJ~YJ3H~L tI I5lit1fIJf5.1hepes5.2NAD5.3D-MDHI !5.4NaClI !5.5KClIDB61!T413~20075.6 Na2HP04.·2H2O5.7lim'.5.8HCl5.9NaOH5.10KOH5.11:m:~*5.121Bj:ijitffljij&litillj~6iii­6.1nRl#t(5mL,1mL50-200ILL10-50IlU6.2~t~.nn.::7\:;~£1.0em6.3~91'~*:7\:;Jlit,r:iJS£iJt*,tE340nmJIJ:AE6.4jJf:ll~~,11~1011m6.5J§iIL',ll6.6~IL'i~~tf,~~jtILn;~*ft~G~;~~,J.&JIii~~7~jmIJ-WiJJ7.1 lit1f1J~ililj7.1.1hepes~;,*,j~1'£90mL:m:~*r:p:5t!tlIJ:JJO/\0.8gNaCL0.037gKCL0.0135gNazHPO,·2H20,0.1g1i1rfJ~,0.5 ghepes,3'EJh~fft¥Jfi,J.j:jNaOH~~pH7.05,100mlo7.1.2 NADI1-t:HiImLl1iJ~*mflllf.52.5rngf1)}&P:1JNADT1t:~,-15C--20·C1:*tfo7.1.3D-MDHI1-t:Rl0.6 mL.ffi:~*?$M8UEr-Jf;f)}~P;k)D1ji:lti:l~Hl;:Jmtl.!W.I~1t~,-15'C--20·C1*#o7.1.4HCl:2mollL7.1.5NaOH:2mollL7.1.6KOH:2mollL7.2 litt$ililji­7.2.11C~§t;~~~;tJ+jKOHllWl~25rnlm:HpHfll~7~8.:J.mm:l.J~iJj~.1im~.1lJ:H1.000L800mL~~iJ11ft@:i1I!uo7.2.2;~~!ll=*5t*$&:i1~¥*1!l:!.ffRn,!'iH:,lfXLm~25ml.mKOHllWl~pH7-8,:JJQM!~*1imff,1fJtJL000~1.800mL;IJt~iJ1lJt@:~W07.2.3 *CO2~f*=*jtf$&~~:t:ffr~i¥JCO~~1*,M~:JJQNaOHl£KOH.i)aJ','ipH7-8.Jt~~ff.kg~15?t~.1fJH1.0001.800mL¥);~~iJ11Tt@:~!~07.3f&JIJ(t~~IltHT)7.3.1ii:£S)!1~~21DB61ff413-2007li'U~~lITI.,*iXJJQA1.OOOmLhepestjftP~,0.100mLNAD11'F~'1.800mL:m:~7l,$15-i:67t~)§~IttJllUtJ3tfll(AI),:P}JJIlAO.050mLD-MDHI.ff~,M'$15-&)S\Z5EJ'&;C:k~1120:7t~),~IttIJ&Ytlim(A.),~aIJ&Ytlim&=(A2-AI)'2807.3.2 ~~~JIJItt!t~lITI.,*iXJJIlA1.OOOmLhepestj{l1l~,O.100mLNADI.fF~,0.100mL~ff~,1.700mL1t~7l$ili:6:7t~)§iiIttIJ&Ytltm(AI),:pJJJIlA0.050mLD-MDHI.fF~,M'$1r&)S\Z5EPX:(*~1120:7t~).~IttIJ&Ytltm(A.),ff~IJ&Ytlim&=(A.-AI)I',lbo~1~i9!IJ'~J¥JJIlA!tellIla9m~hepestjH~NADI.fF~ff~m~:X~~7l~aX1~ff~;fi~g1.000mL1.000mL0.100mL0.100mL-0.100mL1.800mL1.700mL$iI.*£16:7t~J§w;IttIJ&YtJ.tm(AI)oJJIlA~'fm~J§7f~&)s\z0.050mL0.05OmLD-MDHI.ff~:7tJJlJ;Jldff~~~aa9IJ&Ytltm&,fflff~IJ&Ytltm&~~~aIJ&Ytlim.&Iij~1tJJIj0~(]):l:::.A=(A2-AI)1'1iI.-(ArAI)21'3............................................(1)lI)§a9IJ&Ytltf.a&)S\Z*-f-0.100.1f~g1tJJUl1i~1iJrla9~*o8it.:ftHt!l0~(2)it_m&:V/XMWc=---------XL1AXf.....................................................(2)eXdXV2X1000~*:1C~;fi~WoJ9ta9mffl:giLI~;fimlH~J9tI¥J*fFJ9t:l:oD-1fl*D1f:fFJ9t:l::134.09g/mo1,D1fl*D.!f:1f:fFMW132.07g/molVI!t~lITI.*.~m.I¥J~~~,.~~~V2~~ff~~l¥Jff~m.I¥JA~~,.~a~fff~m.a9~~ffr~d!t~lITI.I¥JYt;f.!i!,J(!ffLaeme NADPHl¥JmYt~~:.:tE340nm:=6.3[1xmmor1xem·l]3DB61rr413~20079D-~:!jfU~~~mIJP-ttr.J~~lit~fi9.1D••M~~m~.-~~*tr.JD~••*m.9.2~~tr.JJ2i:m9.2.1;tfi;fjQ~~)~Hfi£IJ&J2i:f*~r:p9.2.2:'!:$JftHEiilHY;E:=:&J2i:ffrfn~H&&J\lZAA*,A2ffiB~Il,tmO.OSOmLR1mtf:l~j]n~IJ:f.&~~nBir~rp,&}\Il~tf;ltJ§(]Omin),~.~OO~~~(Ar~)*W*&~,J\lZ~~.~~fi~m.•~~&m~.~~~.~~~&m*:!Ju.9.2.3~~~~m~~~~~,~~.~~~M~*li~.,~~ffMM~rp~NW~.~tto*2~~iit~~i1¥R1m~hepes~J~1fl~A1.000mLff:rfn1.000mLfjf(ff.........-~1.000mLt+Mt+*$f1£1.000mLNADTft=;1&O.lOOmLO.lOOmLO.lOOmLO.lOOmLn'u~~-nn.O.lOOmLO.050mL)JfH~~~H&O.lOOmLO.050mL1.800mL1.700mL1.700mL1.700mLitU3£~ft.------­x100[%]...............................................(3)LlA/,iff4ItDB61tr413-2007fIJfj:iF;At(~*4't11Jfj:iF;);iJ:*J!qf)2,ff1litl~S9fltitP~,!WJtU:%T~~J5IlffT2·C'-S·C.i1!ic1li1jpxS9NADIft~tE2C--8'CPJ~)EfrI=ffd,eX;tE-15'C¥-25·C*fttrPJ1*ff.=::1'-JiQi1!iC1IitlnZS9D-MDHItF~tE2·C~8'CPJ~)E5:R..~i)(.IIIfjA:ff~S9~~,~~m:~*~~,~:ff~~~Sm~S9.~~~~~.~1II5,tL~j!j9JUJt~U~7tJJtflIt!HAi8/J\(tLOO.100),)JlIJ:ff~m~J5Ilm:mr5ltJl,!!PtE7.3.2~J)EJ:f:lilJ;lJoI1!!IIIIIIJtI,IIIIiI•f,r,ttI!iI!,!5II

1 / 7
下载文档,编辑使用

©2015-2020 m.777doc.com 三七文档.

备案号:鲁ICP备2024069028号-1 客服联系 QQ:2149211541

×
保存成功