人胃癌组织块裸鼠原位移植

ö1,1,1,2,2,2:,ö,,,()100%100%90%75%14,ö,:;;;:R735.2:A:100028578(2001)0120015202:2000201206;:2000204214:(95052):1.210029,2.EstablishmentofOrthotopicImplantöMetastaticModelofHumanStomachCancerusingIntactTumorTissueinNudeMiceLIUPing,ZHAOZhi2quan,ZHANGXiao2yong,etalDepartmentofDigestivedisease,TheFirstAffliatedHospital,NanjingMedicalUniversity,Nanjing210029,ChinaAbstract:ObjectiveTodevelopnudemouseorthotopicimplantömetastaticmodelofhumangastriccan2cerusingintacttumortissue.MethodsTumorcelllineofhumangastricadenocarcinomawasinoculatedsubcutaneouslyintonudemicetodevelopimplant2tumor.Intacttumortissuewasthencollectedandim2plantedorthotopicallyintothenudemousestomach.Thelocaltumorgrowthcharacteristics,tumor2takeratesandmetastasisrateswereexamined.ResultsA100%tumor2takeratewasobtainedinthemodel.Themetastasesoflocalanddistantlymphnodeswereobservedin100%and90%ofthenudemicerespectivce2ly.Thelivermetastaseswerefoundin75%ofthenimals.Themediansurvivaltimeofthetumor2bearingnudemicewas14weeks.Emacicationandexhaustionofthenudemicewerepresentedinlatestageoftheexperiment.ConclusionThegrowthandmetastasischaracteristicsofhumanstomachcancerwereexhibitedintheorthotopicimplantömetastaticmodel.Thistumormodelmightbeausefultoolintheresearchesonmetastaticmechanismsandantimetastasistreatmentofhumanstomachcancer.Keywords:Stomachneoplasms;Tumor2cells;Diseasemodel;Nudemouse,23%,ö,11.1BALBöc(nuönu)20,,610,1823,SPF1.2SGC27901(),10%RPMI21640,CO21.3[1]SGC27901,1107ml,0.2ml,1cm,112mm,,(2ö)1.412h,1%(100mgkg-1),,,4,W23mm,5-0512001281,,,1.51.5.1,,,1.5.2,;;();10%,,HE,,22.1323mm,,56,10,1015mm,,,,,,1118,14100%2.22.2.1,,,,,,,,,2.2.2,,,(1)[1],,(2,3),,(4)(1)2.3,1120ö20(100%)20ö20(100%)18ö20(90%)15ö20(75%)3,,ö,,[1-3],,,,,,[4],[3-6],,,ö,[4,5],,,,,[4,5,8],-,,(2):[1],,,.[J].,1998,18:1012103.[2],,,.[J].,1994,14:2372239.[3]FidlerIJ.Criticalfactorsinthebiologyofhumancancermetas2tasis:Twenty2eighthG.H.A.ClowesMemorialAwardLec2ture[J].CancerRes,1990,50:613026138.[4]FurukawaT.,FuX.,KubotaT.etal.Nudemousemetastaticmodelsofhumanstomachcancerconstructedusingorthotopicimplantationofhistologicallyintacttissue[J].CancerRes,1993,53:120421208.[5]FuX,BestermanJM,MonosovA,etal.Modelsofhumanmetastaticcoloncancerinnudemiceorthotopicallyconstructedbyusinghistologically2intactpatientspecimens[J].ProcNatlAcadSciUSA,1991,88:934529349.[6],,,.[J].,1996,18:1092112.[7]HoffmamRM.Three2dimensionalhistoculture:originsandap2plicationsincancerresearch[J].CancerCells,1991,3:86292.()612001281