常见血液肿瘤FISH检测小册

FISH.FISHFISHFluorescenceinsituhybridization-FISH.MICMMorphologyImmunologyCytogeneticsMolecularMICM(Morphology)(Immunology)IHCFCM(Cytogenetics)FISH(Molecular)PCRDNA.FISHFISHNCCNFISH10060MDSMMFISHFISHMDS5qFISHFISHPCR.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.FISHFISHBCR/ABLDFGSPBCRGSPABL1IGHGSPIGHMYCGSPMYCCSP8P53GSPP53CSP17*R*G*F1.AMLFISHAML1/ETOPML/RARACBFBMLLAML1/ETOM2b1AML1/ETOM220%-40%M2b90%M2bM1M42AML1/ETO90%550%-70%PML/RARAM31PML/RARA95%APLM3AML9%2ATRAPML/RARAPMLRARAAPLATRAAPL90%-95%70%CBFBM4E01CBFBAMLAML5%-10%23%M4AML-M4E0M2M5M4CBFBM4E02CBFBAMLMLL1(AML)M4/M52t(911)t(1011)MLLAMLMLL2.CMLFISHBCR/ABLBCR/ABLCML1t(922)(q34q11)BCR/ABLCML95%CMLt(922)(q34q11)5%CMLPhPh+CML2BCR/ABL(Ph+CML)3PhCMLPhFISHBCR/ABL3.HEFISHPDGFRAPDGFRBFGFR12008WHOPDGFRAPDGFRBFGFR1Myeloid/lymphoidneoplasmswitheosinophiliaassociatedwithrearrangementsofPDGFRA,PDGFRB,orFGFR1PDGFRAPDGFRBFGFR1BCR/ABLimatinib4.ALLFISHETV6TEL/AML1TCF3E2ABCR/ABLKMT2AMLL41017ALL80%-90%ALLETV6TEL/AML1ETV6TEL/AML1ALL20%-25%ALLETV6TEL/AML1ALL5EFS89%97%TCF3E2A3%-5%ALLE2ATCF3E2A/PBX1ALL20%-25%FISHBCR/ABLt(922)BCR/ABL25%ALL2-10%ALLt922BCR/ABL5EFS28.6%MLLMLL5%-10%ALL79%t(411)(q21q23)MLL/AF4(41%)5EFS26.7%41017ALL25%41017410177EFS90%5.CLLFISH13q-+1217p-11q-30%B90%BCLLCLL22%CLLB50%CLLFISHCLL75%-80%13q-+1211q-17p-14q326q-13q-D13S25RB113qCLL40%-60%CLLdel(13q14)BinetA13392+1211q-+12CSP12+12B-CLL15%-35%+1250%SmIgFMC7Binet+1217p-P53/CSP177%-11%CLL17pP53(BinetBC)()17p-CLLP53P53P53P5311qATM11q-CLL5%FISH20%ATM12%-20%ATMATMATMCLL6.MDSFISH5q-7q-20q-+8-Y40%-70%MDS95%MDS(t-MDS)MDSMDS,MDSCC31%-49%MDSFISH79%MDSMDS5q-5q-MDS()MDS5782020.8%19.2%16.9%6.4%MDSMDS(-7)1997(IPSS)MDS3(1)7(2)5q-20q--Y(3)+85q-TERT/5q33TERT/5q315qAMLMDS5q5q31-q3310-15%MDS5MDS40%-5/5q--5/5q-MDS-7/7q-D7S486/CSP7D7S522/CSP777qMDS5-10%AMLM4M615%MDS40%MDS50%AML/MDS-7/7q-AML20q-D20S10820qMPD/MDS4%/AM1%-20/20q-+8CSP8+8MDS+821.1%10%FABMDSIPSS7.MMFISH13q-17p-1q21IGH10%MMWHO2001MM200360%MMMMMM+3+5+7+9+11+15+19+2l-8-13-14-17-22MM1(1p1q)13q-14q(14q32)15%-20%FISH38%-54%13q-D13S319RB113MMMMFISH30%-50%RB142.3D13S31942.3P53P53MM5%-10%P5324.7P53del17pASCT1q211q21CKS1BMMCKS1B1q21MMIGH40-60%MMIGHIGH11q13(BCL1/CCND1)4p16.3FGFR316q23(MAF)20q11(MAFB)6p21(CCND3)t(1114)(q13q32)t(414)(p16q32)t(1416)(q32q23)20%15%5%t(1114)(q13q32)t(414)(p16q32)8.FISHCCND1/IGHBCL2/IGHMYCBCL6MALT1CCND1/IGHt(1114)CCND1/IGHIGHCyclinD195%MCLt1114q13q32MCLCLL/SLLBCL2/IGHt(1418)BCL2/IGHIGHBCL2t(1418)FL93%MYC80%BLt(814)(q24q32)15%BLt(28)(p11q24)5%t(822)q24q11MYCBLBLBBCL6BCL630%-40%BDLBCLBCL6DLBCLBCL6BCL2DLBCLMALT1MALTMALTt(1118)(q21q21)/API2-MALT1t(1418)(q32q21)/IGH-MALT1t(114)(p22q32)/IGH-BCL10(371218)/MALTMALT118q21MALTIGHIGH50%BNHL50ALK60%-85%ALCLALK2ALKALKALK5ALK70%vs30%ALKWHOALCL.FISH2-3ml3-5ml22-872h