《分子生物学》习题答案

guu900
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2021-11-18

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11.Waston1933Watson1953DNA_DNA2.DNARNAmRNAsiRNADNAdeoxyribonucleicacidRNAribonucleicacidmRNAmessengerRNARNAtRNAtransferRNARNArRNAribosomalRNARNAsiRNAsmallinterferingRNARNA3.4.DNA1)SRSSRS+RS+RSDNAR*DNA2)35S+1-235S32N+1-230%32N*DNA5.DNADNA6.DNA2DNA1.2.H2AH2BH3H4200bpDNADNAH13.3DNADNATmDNADNADNARNADNADNADNADNaseDNADNADNA200bp4.H3H4N/DNAXADP5.DNADNA4DNADNADNADNAkink6.DNADNADNADNADNARNAmRNAmRNADNA7.DNAWatsonCrick195315NH4Cl1415NDNA14NH4Cl14N14NDNA15NDNADNA,CsClDNADNADNADNADNA14N15N14N15NDNADNA14N15NDNAgeneration1.9generation114NDNADNADNA208.DNADNADNADNADNA9.DNADNADNADNA10.DNATmTmDNADNADNAG+CG+CDNATmDNATmTmDNADNADNA11.DNADNADNA5335DNA5335DNA53DNADNA53DNA4101000~2000DNA535DNADNA53DNA12.DNAG1SS13.DNADNADNASOSDNA14.DNAIS15.SNPSNPSNPDNAATCGSNP30300SNPsSNPSNPSNP3--DNARNA1.mRNADNAmRNADNA2.RNAmRNARNAtRNARNAmRNArRNARNARNARNA3.RNARNARNARNA4.RNARNADNARNARNA---5.DNADNARNA,AU,AT6.RNARNA2111RNA7.RNADNADNANTPRNADNARNA8.RNADNA9.PribnowboxRNA10bpTATA-10TATAAT10.PribnowPribnowTATGTTTATATTPribnowTATAATAATAAT11.mRNAmRNAmRNAmRNAmRNAmRNAmRNA53polyAmRNA5A12.RNARNARNA,.,,.13.GCDNARNA4~8A3URNApoly(U)GCRNADNA14.mRNA53polyAhnRNADNADNA15.GMPGDPGTP3-OH5RNA3-OH3rRNA32-OH5RNA3-OH316.RNARNA2,5-mRNA17.RNARNARNAmRNADNARNAmRNADANRNA3RNA18.RNasePRNAM1RNARNARNA311~13RNARNA4--mRNA1.2.3.3UAAUGAUAGUAAUAGUGA4.tRNAtRNAtRNA3CCA-OHtRNAtRNAAUGCtRNAtRNAAPtRNAL5.tRNA1tRNAAC1GU2I3tRNA6.tRNAmRNAATPtRNAAA-tRNAAA-tRNAtRNAmRNAtRNAAA-tRNAmRNA-7.RNARNAmRNARNA2/31/3RNA3/52/58.SDSDmRNA5-AGGAGGU-330S16SrRNA35-GAUCACCUCCUUA-3SDmRNA9.mRNAAA-tRNAA-tRNAP10.mRNAm7GpppNpMet-tRNAMetmRNA53A11.fMet-tRNAmRNA12.13.14.RNA310~15N1C15.16.CSUMOSUMODNA17.18.X1-3X2-3X,LIFMXCRM151.PCRDNADNADNAdNTPDNADNA2.SYBRGreenTaqManSYBRGreenTaqManDNA3.DNAcDNADNADNAcDNAmRNAcDNA4.cDNAEcoRcDNAXhocDNA53EcoRXho5.SDS-PAGE71.mRNA2.lacZlacYlacAmRNAmRNAPlacIODNA26bplacmRNAlacmRNAmRNA3.4.tRNATrp41Trp2-33-4trp422-33-4-trp5.RNARNARNA-6.RNARNAmRNAmRNAmRNA7.mRNARNA81.2.mRNAPolyAmRNA3.DNADNADNADNADNADNADNA4.RANTFRNA5.DNA3DNADNADNADNARNADNARNA6.:DNADNA7.T-DNA8.9.CTDS2S510.activationtagging11.cAMPAATPcAMP12.NH2A,H2B,H3,H413.14.DNA