三代基因组测序技术简介及其原理整理.

DNA1975SangerCoulson1976-1977MaxamGilbert1977X1745375Sanger2001SangerSanger1DNAdNTPN=A/G/T/C3-OH4DNAddNTPDNAddNTP23dNTP2DNADNADNASangerDNA45DNADNADNA1000bp99.999%Roche454illuminaSolexaHiseqABISolid31Roche454Roche4541DNA454illuminaDNA300-800bpDNAPCRDNA2EmulsionPCRPCR454DNAilluminaDNA28umPCRDNAPCRPCRPCRDNADNADNAPCRPCRDNA100DNA3DNAPTP44umPTPDNAdNTPdNTPATPATPATPPTPCCDdNTPdNTPATP454PTP454454400bpPolyATT454SolidSolidABI2007DNA62,41DNADNA2EmulsionPCRSolidPCR454emulsionPCR4541um33148Solid4543SolidDNASolid83-XXnnnzzz-5DNA5CY5TexasRedCY36-FAM4812XX6-8zzzSolidDNA126-a6-b125651267n-1n-1nn-1n30156250bp99.94%15x99.999%IllumineIlluminaSolexaHiseq2,441DNADNA200-500bpDNA2FlowcellFlowcellDNADNAflowcellflowcellchannelFlowcell8channelchannelDNAflowcellDNADNAPCR3PCRPCRFlowcell4.aDNADNA4DNA4dNTPSangerdNTP3-OHdNTPdNTPdNTPDNAdNTP3-OHIlluminadNTP1%-1.5%30x1PacBioSMRTOxfordNanoporeTechnologiesPCRPacBioSMRT5SMRTDNA,44dNTP,,,,DNA,,PacBioSMRTZMW,,,,,(SMRTCell:),ZMW(),100,(),,(20X10-21L),,,,SMRT10dNTP15%,OxfordNanoporeTechnologies5DNA8(AGBT)kb100kb;1%4%;;(30x);DNA;RNAbisulfite99.8%IonTorrent6DNADNAPHH+H+DNA454JonathanRothberg454454H+IonTorrent22-3.510G