TUNEL法检测细胞凋亡实验原理和方法

TUNELDNADNA50-300kb30DNACa2Mg2180200bpDNADNADNA3-OHTdTDNA3-TUNELDNA3-OHDNADNAnicktranslationDNATUNEL[digoxigenin-11-dUTP]TdTDNA3-OHdATP1FcFc()1PBSpH7.450mMNaCl200mM2K200g/mlpH7.4K0.02gPBS100ml32%H2O2PBSpH7.4H2O22.0mlPBS98.0ml4TdTTrlzma3.63g0.1NHClpH7.2ddH2O1000ml2996g0.238g5TdTTdT32lTdT76l617.4g8.82gddH2O1000ml70.05DABDAB5mgPBS10mlpH7.4,0.02%80.5pH4.00.5g0.1M100ml9100100959080701010ONCOR115min3min95753minPBS5minK20ugml15min42min221010minPBS5min21-205minPBS5min235107/ml410min50100PBS5min222PBS5minPBS5min32TdT15min454TdT37C1hrTdT5373730min10min6PBS35min30min7PBS45min80.05DAB36min9431min15min1010min310130s1001132minDNaseI1M3-4hrTdT7G22++ThermoRoche6:DNAHRMRT-PCRPCRrealtimeQPCRISH6:TUNELELISAWesternblottingIHCEMSAChIP10:siRNADNA