动物肝脏中DNA的提取及检测实验报告

DNADNADeoxyribonucleicacidDNADNADNADNA260nmDNADNADNADNADNA46G1-DNAS-DNAG2-DNADNADNADNADNADNAdAMPdTMPdCMPdGMPDNADNAmRNARNADNA35-DNADNAX174G4M13DNADNADNADNA5-DNA5-5-40E.ChargaffDNAA=TG=CDNADNADNP/RNPDNP0.14MRNPNaClDNPSDSDNA-DNA2550005020-30/()6-8:1.DNADNP/RNPDNP0.14MRNPNaClDNPSDSDNA-DNA0.1mol/LNaCl-0.05mol/LpH6.895%A.R.NaClA.R.5%SDS5gSDS100mlV()V2011.20.1mol/LNaCl-0.05mol/L2.DNA4ml104000r/min10min8ml4000r/min5min10ml5ml-1mlSDS30minNaCl0.9g1mol/L2104000r/min5min95%DNA8mlDNA10ml3.6045min595nmDNA012345DNA/ml0.00.40.81.21.62.0/ml2.01.61.20.80.40/ml4.04.04.04.04.04.0A595nm4.DNA25mlDNA1.0ml1.0ml4.0ml6045min595nmDNAug5.100gDNAw=m1/m2100%wDNA%m1DNAugm2ug1.012345DNA/ml0.00.40.81.21.62.0/ml2.01.61.20.80.40/ml4.04.04.04.04.04.0A595nm00.0390.090.1460.1970.249DNA/ug0801602403204002.2.04gDNA12.53ml123A595nm0.1020.1020.101A595nm0.102DNA/ug171.4/g0.1628w=m1/m2100%w=0.1053100gDNAw100=0.1053g1.SDS-NaClDNApHSDSDNA-NaClDNARNA1.DNADNADNADNADNADNA2.EDTASDSEDTA3.-4.5.100gDNA0.1053gDNADNADNADNADNADNADNADNADNADNADNADNADNADNADNA