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244Vol.24,No.420027ChineseJournalofPreventiveVeterinaryMedicineJuly2002:(1963),,,,:2001-07-06LAL,,,,(,730030):(TVB-N),LAL(),LALLAL(12),,:;;LAL;:S852.61:A:1008-0589(2002)04-0304-06RapidDeterminationofBacterialContaminationandFreshnessofMeatbyLALTestWUZheng,WANGDeng-lin,DONGRui-peng,YANGHong-yan,ZHANGLei(GansuProvincialAnimalQuarantineStation730030,China)Abstract:Threedifferentfreshnesssamplesofpork,beef,mutton,chickenandfishwereexaminedforthenumbersoftotalviablecells,gram-negativeorganisms,colifromgroupsandthevalueofTVB-N.TheresultswerecomparedwiththoseofLALtest(limulusamoebocytelysatetest).Apositivecorrelationwasobservedamongthetotalviablecells,gram-negativeorganismsandLALindexes.ItindicatedthatLALtestin2dexcanbeusedtodeterminethebacterialcontaminationandfreshnessofthemeatrapidly.Keywords:Meat;Bacterialcontamination;LALtest;Rapiddetermination,,,,(TVB-N),;TVB-N,TVB-N[1],,48,,[1],,,,,,,,,,,B,LAL[2,3]LAL[4],©1995-2004TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.11.12EU/ml,,9807089811031.2;LAL1.3;1.4:28,2;5,11;:5;5,3;:3;3,2;:6;3,2;():6;2,2(37)1.5LAL,0.1ml,,370.5602,,,180LAL(10)1.6,4cm25g,,45ml,1015,1101ml9ml,,1102()11051108LAL1.7TVB-NGB5009.44-851.8GB4789.2-84GB4789.3-841.93,,2,0.2ml,3748,1.10LALTVB-N(r),LAL22.1TVB-NLAL15,88,,,LAL;TVB-N;(TVB-N)(p0.01)TVB-NLAL,(p0.05);(p0.01),2.2LALTVB-V(r),6LALTVB-V(r)0.9550.9480.8130.363;TVB-V5034,.LAL©1995-2004TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.1TVB-NLALTable1Detectionresultofsenseorgan,TVB-N,microbeandLALtestofporkSampleNO.DetectionofsenseorganTAB-NValueofTVB-N(mg/100g)TotalviablecountsG-Gram-negativecounts(logCFU/g)Coliformgroup(logMPN/100g)LALLALindexContentofendotoxin(ng/g)0112.84.03.04.4718029.74.13.04.63180312.84.153.04.6018049.74.233.03.9518056.94.263.05.0418069.74.42.43.62800712.54.543.05.38180814.04.74.05.42800914.04.74.05.42801012.84.753.05.6318118.44.83.03.62801214.05.04.05.42801311.25.04.05.42801412.65.04.05.4280159.85.13.04.22801611.15.13.184.973800176.95.173.103.4818189.85.283.44.322801910.05.384.34.482802010.05.414.455.97280216.95.483.34.9280229.75.483.74.972802311.25.544.05.62802412.55.63.955.66280259.85.854.44.632802611.25.894.344.462802713.65.894.475.4828028Grade6.95.904.665.3828029A22.36.855.487.3838003022.47.116.435.63481033112.57.115.96.3838003220.57.666.724.634810333Grade18.37.686.66.384810334B18.17.766.576.38481033525.07.786.06.63481033636.28.088.08.38681053716.78.257.118.38681043819.58.36.88.38581043944.68.68.388.38681054057.48.88.118.38581044128.68.817.768.38581044237.18.877.46.635810443Deterioration37.18.897.26.63581044431.88.937.08.38581044534.49.256.96.97581044628.69.268.328.38681056032002©1995-2004TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.2TVB-NLALTable2Detecionresultofsenseorgan,TVB-N,microbeandLALtestofbeefSampleNO.DetectionofsenseorganTAB-NValueofTVB-N(mg/100g)TotalviablecountsG-Gram-negativecounts(logCFU/g)Coliformgroup(logMPN/100g)LALLALindexContentofendotoxin(ng/g)474.23.72.03.4718488.73.782.43.4184912.13.92.63.81850grade5.64.382.03.471851A8.44.672.03.45185217.75.04.05.42805315.95.34.05.4185419.25.384.114.7828055Grade21.05.864.45.028056B11.26.364.74.47280576.46.785.05.42805824.07.65.45.8380059Deterioration26.27.85.726.1138003TVB-NLALTable3Detectionresultofsenseorgan,TVB-N,microbeandLALtestofmuttonSampleNo.DetectionofsenseorganTAB-NValueofTVB-N(mg/100g)TotalviablecountsG-Gram-negativecounts(logCFU/g)Coliformgroup(logMPN/100g)LALLALindexContentofendotoxin(ng/g)605.63.92.03.418615.64.62.03.471862GradeA7.04.652.784.3618639.65.04.05.47186412.76.085.05.628065GradeB16.06.485.234.72806618.27.786.114.92380067Deerioration25.28.286.973.97038004TVB-NLALTable4Detectionresultofsenseorgan,TVB-N,microbeandLALtestofchickenSampleNo.DetectionofsenseorganTAB-NValueofTVB-N(mg/100g)TotalviablecountsG-Gram-negativecounts(logCFU/g)Coliformgroup(logMPN/100g)LALLALindexContentofendotoxin(ng/g)6813.32.481.72.6186914.62.481.72.4818709.62.541.73.48187112.72.781.72.618729.52.91.72.61873GradeA15.92.951.72.4818745.43.73.183.48187512.74.03.04.171876GradeB13.35.184.485.62807712.77.56.486.38380078Deterioration9.67.61.66.3838007034,.LAL©1995-2004TsinghuaTongfangOpticalDiscCo.,Ltd.Allrightsreserved.5TVB-NLALTable5Detecionresultofsenseorgan,TVB-N,microbeandLALtestoffishSampleNo.DetectionofsenseorganTAB-NValueofTVB-N(mg/100g)TotalviablecountsG-Gram-negativecounts(logCFU/g)Coliformgroup(logMPN/100g)LALLALindexContentofendotoxin(ng/g)7915.94.02.74.48280805.74.483.03.48280818.94.653.03.48280828.94.653.03.48280837.64.653.03.628084GradeA8.94.853.03.482808512.15.483.884.95380086GradeB11.55.64.484.638008712.16.525.545.634810388Deterioration15.86.574.996.66481036LALTVB-V(r)Table6Correlativitycoefficient()oftotalviablecounts,gram-negativecouts,coliformgroupandTAB-VvaluewithLALindicesofthemeatMeatTotalviablecountsGram-negativecountaColiformgroupTVB-NValueofTVB-NPork0.9530.9510.8450.83BeefandMutton0.9930.9020.5910.388Chicken0.9680.9600.920-0.036Fish0.9670.9780.8960.27033.1LAL(r0.902),,,LAL,LALMisawLAL,LAL[2],LALTVB-N,Sul