光合细菌法降解淀粉废水积累菌体蛋白的研究

ISSN1000-0054CN11-2223/N()JTsinghuaUniv(Sci&Tech),20074732007,Vol.47,No.311/36348-351光合细菌法降解淀粉废水积累菌体蛋白的研究王剑秋1,　管运涛2,1,　滕　飞1(1.,100084;2.,518055):2006-04-05:(50108007):(1981-),(),,:,,E-mail:[e-mail]:为研究紫色非硫光合细菌对于高浓度淀粉废水的处理和菌体蛋白积累效果,采用序批式紫色非硫光合细菌法(PNSB-SBR)处理高浓淀粉废水。经过两个月的运行,在进水淀粉废水化学需氧量(CODcr)浓度为5000mg/L,运行周期为48h,微好氧、恒温30℃光照条件下,出水CODcr浓度为500～1000mg/L,其去除率达到70%～90%。污泥产率Yx,s约为每kgCOD可产出0.4kg可挥发性悬浮颗粒物(VSS),菌体蛋白含量达到30%～50%,同时蛋白质产率Yp,s约为每kgCOD可产出0.2～0.4kg菌体蛋白(SCP),并呈上升趋势,系统运行稳定。结果表明,采用PNSB-SBR工艺能有效处理高浓度淀粉废水,并同时有效地积累菌体蛋白。:紫色非硫光合细菌(PNSB);菌体蛋白(SCP);淀粉废水;序批式反应器(SBR):X703.1:A:1000-0054(2007)03-0348-04Accumulationofsinglecellproteinbypurplenon-sulfurphotosyntheticbacteriainstarchwastewatertreatmentWANGJianqiu1,GUANYuntao2,1,TENGFei1(1.DepartmentofEnvironmentalScienceandEngineering,TsinghuaUniversity,Beijing100084,China;2.GraduateSchoolatShenzhen,TsinghuaUniversity,Shenzhen518055,China)Abstract:Theeffectofchemicaloxygendemand(CODcr)removalandsinglecellprotein(SCP)accumulationduringthetreatmentofhighconcentrationstarchwastewatertreatmentwasinvestigatedusingthepurplenon-sulfurphotosyntheticbacteria-sequencebatchreactor(PNSB-SBR)method.Theresultsindicatethatafter2monthswithmicroaerobicconditions,light,constanttemperature(30),and48hcycles,theCODcrwasreducedfrom5000mg/Lto5001000mg/L,forremovalratesof70%90%.Yx,s(yieldofactivebiomassonthesubstrate)reached0.4kgVSSperkilogramofCODforcellproteinconcentrationsof30%50%.Yp,s(yieldofSCPonthesubstrate)was0.20.4kgSCPperkilogramofCODandslowlyincreasing.TheresultsshowthatthePNSB-SBRmethodcaneffectivelytreathigh-concentrationstarchwastewaterwhileaccumulatingSCP.Keywords:purplenon-sulfurphotosyntheticbacteria;singlecellprotein;starchwastewater;sequencebatchreactor(singlecellprotein,SCP)[1](purplenon-sulphurphotosyntheticbacteria,PNSB),,[2]PNSB,[2],PNSB60%[3],Q[3],[2],PNSB,;(sequencebatchreactor,SBR),,,[1-4],SBR,,1　试验材料与方法1.1PNSBVanNielPNSB[5,6]5PNSB,30[2]1.2[4],CODcr5000mg/L,pH6711/(gL-1)5.000.75(NH4)2SO40.05KH2PO40.03NaCl1.00NaHCO31.00MgSO40.09CaCl20.031.3SBR,110mm,550mm,5L,3L,[1,2,4,7,8]:,30,60W10cm,,(dissolvedoxygen,DO)0.42.0mg/LpH69,3L,(sludgeretentiontime,SRT)6.7dCODcr6500mg/L300h,48h,CODcr111.4CODcr,[2];,OD[9];,[2,3];pH,(ORIONModel828);DO,(ORIONModel810)2　结果与讨论2.12(FEIQUANTA200),,,,:[2]22.21.5g/L,20%,CODcr6500mg/L,300hCODcr33CODcrCODcr3,300h,CODcr6.5g/L0.5g/L,CODcr90%CODcr349,:,72h50%,100hCODcrODMLSS-OD(MLSS),OD660MLSS[MLSS]=1.0224OD660(R2=0.978),OD100h0.42.0,1.9,,PNSB,2.32.3.1　CODcr去除效果,CODcr5g/L,CODcr44CODcr4,,CODcr4.05.0g/L,CODcr0.51.0g/L,,CODcrCODcr70%90%BOD55CODcr(BOD55)0.6[9],BOD50.9kg/(m3d),kgMLSS0.55kgBOD5,CODcr,0.42.0mg/L,PNSB,,,PNSB,,PNSB,,,PNSB2.3.2　蛋白质PNSB,;,,[6],CODcr,56,6VSS56CODcr-/5,,,40d30%50%,OD1.52.0,40d40d6CODcr()CODcr(),6,350()2007,47(3),35d,Yx,skgCODVSS0.4kg,Yp,skgCODSCP,3　结　论,PNSB-SBR,1)PNSB-SBR48h,,,CODcr5g/L,CODcr70%90%,2)PNSB-SBRPNSB,Yx,skgCODVSS0.4kg,Yp,skgCODSCP0.20.4kg,30%50%,,3),,,PNSB,,4)SBR,,,,,,(References)[1],.[J].,2005,26(17):45-48.CHANGQiaoling,ZUOQianhai.Exploitationofproteinresourcesusingorganicsewage[J].FeedIndustry,2005,26(17):45-48.(inChinese)[2].PHB[D].:,2004.WUGuangxue.PHBAccumulationbyPurpleNon-SulfurPhotosyntheticBacteriaandActivatedSludge[D].Beijing:DepartmentofEnvironmentalScienceandEngineering,TsinghuaUniversity,2004.(inChinese)[3],,,.PHB[J].,2004,25(6):102-107.WUGuangxue,GUANYuntao,WANGJianqiu,etal.ThestudyabouttheeffectofcarbonandnitrogensourcesontheaccumulationofPHBbypurplenon-sulfurphotosyntheticbacteria[J].EnvironmentalScience,2004,25(6):102-107.(inChinese)[4],,,.SBR[J].(),2004,43(3):376-380.LIAOXinkai,LIQingbiao,CHENWenmou,etal.ProcessconditionsforsyntheticstarchwastewatertreatmentbySBRmethod[J].JournalofXiamenUniversity(NaturalScience),2004,43(3):376-380.(inChinese)[5].[M].:,1991.LIURulin.CharacteristicandApplicationofPhotosyntheticBacteria[M].Beijing:ChinaAgriculturalScientechPress,1991.(inChinese)[6].,CO2[D].:,1997.NAKAJIMAFumiyuki.InfluenceFactorofPurpleNon-sulfurPhotosyntheticBacteriainOrganicProduction,CO2UptakeandBacteriaComponentChanginginWastewaterTreatmentProcess[D].Japan:TokyoUniversity,1997.(inJapanese)[7]CodgellRJ,SouthallJ,GardinerAT,etal.Howpurplephotosyntheticbacteriaharvestsolarenergy[J].ComptesRendus-Chimie,2006,9(2):201-206.[8]CarlozziP,SacchiA.BiomassproductionandstudiesonRhodopseudomonaspalustrisgrowninanoutdoor,temperaturecontrolledunderwatertubularphotobioreactor[J].JournalofBiotechnology,2001,88(3):239-249.[9],,,.[J].,2005,24(7):23-25.ZHANGHonglian,XIALiqun,LIUYibing,etal.Studyonenrichmentsubstrateofphotosyntheticbacteria[J].FisheriesScience,2005,24(7):23-25.(inChinese)351,: