当前位置:首页 > 行业资料 > 国内外标准规范 > pET-System-Manual
UserProtocolTB055Rev.C0611JNPage1of63USAandCanadaEuropeAllOtherCountriesTel(800)628-8470biosciencehelp@emdchemicals.comFranceFreephone0800126461GermanyFreecall08001003496IrelandTollFree1800409445UnitedKingdomFreephone0800622935AllotherEuropeanCountries+441159430840ContactYourLocalDistributor@emdchemicals.com————————————————techservice@merckbio.eu————————————————®QuickScreenforExpressionLevel,Activity,andSolubility18B.TotalCellProtein(TCP)Fraction18C.MediumFraction18D.PeriplasmicFraction19E.SolubleCytoplasmicFraction20F.InsolubleCytoplasmicFraction21InclusionbodypurificationafterBugBuster®MasterMixtreatment22InclusionbodypurificationaftermechanicalcelllysisorrLysozyme™treatment22G.PreparationofExtractswithPopCulture®Reagent22H.DetectingandQuantifyingTargetProteins23VI.PurifyingTargetProteins.............................................................................................25A.ProteinPurification25B.SolubilizationandRefoldingProteins25VII.AdditionalGuidelines..................................................................................................26A.ChoosingapETVector26B.pETVectorCloningStrategies30C.RegulatingProteinExpressioninthepETSystem31USAandCanadaTel(800)628-8470biosciencehelp@emdchemicals.comGermanyTel08001003496techservice@merckbiosciences.deUnitedKingdomandIrelandUKFreephone0800622935IrelandTollFree1800409445customer.service@merckbiosciences.co.ukAllOtherCountries@emdchemicals.comUserProtocolTB055Rev.C0611JNPage2of63D.HostsforCloning33E.HostsforExpression33VIII.OptimizingExpression...............................................................................................39A.InductionControls39B.EnhancingSolubilityandFolding40C.CorrectingforRareCodonUsage41D.ToxicGenesandPlasmidInstability42E.Coexpressionoftargetproteins43F.OtherFactorsInfluencingExpression44IX.Acknowledgements......................................................................................................45X.References...................................................................................................................46XI.Index............................................................................................................................50XII.Academic&Non-profitLaboratoryAssuranceLetter.................................................52XIII.BacterialStrainNon-DistributionAgreement.............................................................53XIV.Appendix:pETSystemRelatedProducts.................................................................54A.pETSystemHostStrainsandLambdaPhage54B.Detection/AssayToolsforFusionTags59C.PurificationTools61USAandCanadaTel(800)628-8470biosciencehelp@emdchemicals.comGermanyTel08001003496techservice@merckbiosciences.deUnitedKingdomandIrelandUKFreephone0800622935IrelandTollFree1800409445customer.service@merckbiosciences.co.ukAllOtherCountries@emdchemicals.comUserProtocolTB055Rev.C0611JNPage3of63®2011EMDChemicalsInc.,anaffiliateofMerckKGaA,Darmstadt,Germany.Novagen®,Fractogel®,Benzonase®,MagPrep®,BugBuster®,His•Bind®,His•Tag®,HSV•Tag®,NovaTope®,PelletPaint®,PopCulture®,andT7•Tag®areregisteredtrademarksofMerckKGaA,Darmstadt,Germany.BetaRed™,Clonables™,ColiRollers™,DsbA•Tag™,DsbC•Tag™,Ekapture™,FRETWorks™,GST•Bind™,GST•Mag™,GST•Tag™,His•Mag™,LICDuet™,LumiBlot™,Lysonase™,Mobius™,NovaTaq™,Nus•Tag™,OnEx™,OvernightExpress™,PerfectDNA™,PerfectProtein™,pETBlue™,pETcoco™,RoboPop™,Rosetta™,RosettaBlue™,Rosetta-gami™,S•Tag™,Singles™,SpinPrep™,Strandase™,TrailMix™,TriEx™,Trx•Tag™,Tuner™,andXarrest™aretrademarksofMerckKGaA,Darmstadt,Germany.Sonifier®isaregisteredtrademarkofBransonInstruments,Inc.MacroPrep®isaregisteredtrademarkofBio-RadLaboratories,GmbH,Ltd.Cy5™isatrademarkofGEHealthcare.Strep•Tac
本文标题:pET-System-Manual
链接地址:https://www.777doc.com/doc-1449903 .html